##gff-version 3
##sequence-region BBa_M39412 1 1347
BBa_M39412	.	ribosome_entry_site	1158	1169	.	+	0	ID=BBa_J61103;Name=BBa_J61103
BBa_M39412	.	terminator	1	129	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M39412	.	terminator	89	129	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M39412	.	stem_loop	96	115	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M39412	.	polyA_site	116	129	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M39412	.	stop_codon	122	122	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M39412	.	engineered_region	89	129	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M39412	.	terminator	1	80	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M39412	.	stem_loop	12	55	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M39412	.	engineered_region	1	80	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M39412	.	terminator	1219	1347	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M39412	.	terminator	1307	1347	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M39412	.	stem_loop	1314	1333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M39412	.	polyA_site	1334	1347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M39412	.	stop_codon	1340	1340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M39412	.	engineered_region	1307	1347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M39412	.	terminator	1219	1298	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M39412	.	stem_loop	1230	1273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M39412	.	engineered_region	1219	1298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M39412	.	ribosome_entry_site	829	840	.	+	0	ID=BBa_J61106;Name=BBa_J61106
BBa_M39412	.	ribosome_entry_site	849	860	.	+	0	ID=BBa_J61105;Name=BBa_J61105
BBa_M39412	.	promoter	1069	1106	.	+	0	ID=BBa_I14033;Name=BBa_I14033
BBa_M39412	.	promoter	1095	1100	.	+	0	ID=annotation1026173;Name=-10;Parent=BBa_I14033
BBa_M39412	.	promoter	1072	1077	.	+	0	ID=annotation1026172;Name=-35;Parent=BBa_I14033
BBa_M39412	.	operator	1069	1106	.	+	0	ID=annotation1026174;Name=P(Cat);Parent=BBa_I14033
BBa_M39412	.	sequence_feature	1115	1149	.	+	0	ID=BBa_K137008;Name=BBa_K137008
BBa_M39412	.	sequence_feature	1133	1149	.	+	0	ID=annotation1963842;Name=IRR in;Parent=BBa_K137008
BBa_M39412	.	sequence_feature	1115	1131	.	+	0	ID=annotation1963841;Name=IRR out;Parent=BBa_K137008
BBa_M39412	.	terminator	932	1060	.	+	0	ID=BBa_B0015;Name=BBa_B0015
BBa_M39412	.	terminator	1020	1060	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_M39412	.	stem_loop	1027	1046	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_M39412	.	polyA_site	1047	1060	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_M39412	.	stop_codon	1053	1053	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_M39412	.	engineered_region	1020	1060	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_M39412	.	terminator	932	1011	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_M39412	.	stem_loop	943	986	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_M39412	.	engineered_region	932	1011	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_M39412	.	CDS	136	820	.	+	0	ID=BBa_C0040;Name=BBa_C0040
BBa_M39412	.	engineered_tag	796	820	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_M39412	.	CDS	139	755	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_M39412	.	tag	756	789	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_M39412	.	sequence_feature	889	923	.	+	0	ID=BBa_K137010;Name=BBa_K137010
BBa_M39412	.	sequence_feature	889	905	.	+	0	ID=annotation1963843;Name=IRL in;Parent=BBa_K137010
BBa_M39412	.	sequence_feature	907	923	.	+	0	ID=annotation1963844;Name=IRL out;Parent=BBa_K137010
BBa_M39412	.	tag	1178	1210	.	+	0	ID=BBa_M0050;Name=BBa_M0050
BBa_M39412	.	tag	1178	1210	.	+	0	ID=annotation1958880;Name=WT SsrA tag AANDENYALAA;Parent=BBa_M0050
BBa_M39412	.	ribosome_entry_site	869	880	.	+	0	ID=BBa_J61104;Name=BBa_J61104
>BBa_M39412
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatgtccagattagataaaagtaa
agtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagcta
ggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttag
ataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttag
atgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctc
gaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcatt
ttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtat
gccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattg
atcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagctt
aataacactgatagtgctagtgtagatcactactagagaaagataggagatactagagaaagacatgacgtactagaga
aagaagggacatactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagccaggcatcaaataaaa
cgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactg
gctcaccttcgggtgggcctttctgcgtttatatactagagggcacgtaagaggttccaactttcaccataatgaaaca
tactagagaagatgaaacatttggggccaaactgtccatattatactagagaaagattagacatactagaggctgctaa
cgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctt
tcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtt
tata