BBa_M39412
type: ComponentDefinition
persistent identity: BBa M39412
display id: BBa_M39412
version: 1
was generated by: iGEM to SBOL conversion
description: DNAseI generator inhibited by recombinant pCat promoter
created: 2012-05-14T11:00:00Z
modified: 2015-05-08T01:14:07Z
owned by: james
owned by: myers
mutable description: DNAseI generating module that is inhibited by (lack of) recombination. Initially, the promoter is set to produce actin with a fast degradation tag, which inhibits DNAseI. When fimE is expressed elsewhere in the cell, the promoter is flipped to stop production of actin. Instead, DNAseI is expressed, along with ECP32 which degrades actin. Additionally, tetR is produced to be used in other regulatory mechanisms.
discontinued: false
dominant: false
group u list: _1206_
m user id: 0
owner id: 13164
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: There is a terminator before the pCat promoter to prevent "backwards" expression, which would be detrimental to the strong "digital" behavior the recombinant region attempts to achieve. ** Everything before the fimE IRL should be on the antisense strand, and thus inverted.
star: false
creator: Zach Banks
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J61103
component: BBa_K137008
component: BBa_B0015
component: BBa_M0050
component: BBa_K137010
component: BBa_J61104
component: BBa_I14033
component: BBa_C0040
component: BBa_J61105
component: BBa_J61106
sequence annotation: BBa_B0015
sequence annotation: BBa_C0040
sequence annotation: BBa_J61106
sequence annotation: BBa_J61105
sequence annotation: BBa_J61104
sequence annotation: BBa_K137010
sequence annotation: BBa_I14033
sequence annotation: BBa_K137008
sequence annotation: BBa_J61103
sequence annotation: BBa_M0050
sequence: https://synbiohub.org/public/igem/BBa_M39412_sequence/1

BBa_J61103
type: Component
persistent identity: component2312582
display id: component2312582
version: 1
top level: BBa_M39412
definition: BBa_J61103
access: public

BBa_K137008
type: Component
persistent identity: component2312581
display id: component2312581
version: 1
top level: BBa_M39412
definition: IRR
access: public

BBa_B0015
type: Component
persistent identity: component2312591
display id: component2312591
version: 1
top level: BBa_M39412
definition: BBa_B0015
access: public

BBa_M0050
type: Component
persistent identity: component2312584
display id: component2312584
version: 1
top level: BBa_M39412
definition: LAA
access: public

BBa_K137010
type: Component
persistent identity: component2312567
display id: component2312567
version: 1
top level: BBa_M39412
definition: fimE IRL
access: public

BBa_J61104
type: Component
persistent identity: component2312564
display id: component2312564
version: 1
top level: BBa_M39412
definition: BBa_J61104
access: public

BBa_I14033
type: Component
persistent identity: component2312578
display id: component2312578
version: 1
top level: BBa_M39412
definition: Cat
access: public

BBa_C0040
type: Component
persistent identity: component2312561
display id: component2312561
version: 1
top level: BBa_M39412
definition: tetR
access: public

BBa_B0015
type: Component
persistent identity: component2312574
display id: component2312574
version: 1
top level: BBa_M39412
definition: BBa_B0015
access: public

BBa_B0015
type: Component
persistent identity: component2312557
display id: component2312557
version: 1
top level: BBa_M39412
definition: BBa_B0015
access: public

BBa_J61105
type: Component
persistent identity: component2312563
display id: component2312563
version: 1
top level: BBa_M39412
definition: BBa_J61105
access: public

BBa_J61106
type: Component
persistent identity: component2312562
display id: component2312562
version: 1
top level: BBa_M39412
definition: BBa_J61106
access: public

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2312557
display id: annotation2312557
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312557/range2312557/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M39412/annotation2312557/range2312557/1
type: Range
persistent identity: range2312557
display id: range2312557
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1
end: 129
orientation: inline

BBa_C0040
type: SequenceAnnotation
persistent identity: annotation2312561
display id: annotation2312561
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312561/range2312561/1
component: BBa_C0040

https://synbiohub.org/public/igem/BBa_M39412/annotation2312561/range2312561/1
type: Range
persistent identity: range2312561
display id: range2312561
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 136
end: 820
orientation: inline

BBa_J61106
type: SequenceAnnotation
persistent identity: annotation2312562
display id: annotation2312562
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312562/range2312562/1
component: BBa_J61106

https://synbiohub.org/public/igem/BBa_M39412/annotation2312562/range2312562/1
type: Range
persistent identity: range2312562
display id: range2312562
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 829
end: 840
orientation: inline

BBa_J61105
type: SequenceAnnotation
persistent identity: annotation2312563
display id: annotation2312563
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312563/range2312563/1
component: BBa_J61105

https://synbiohub.org/public/igem/BBa_M39412/annotation2312563/range2312563/1
type: Range
persistent identity: range2312563
display id: range2312563
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 849
end: 860
orientation: inline

BBa_J61104
type: SequenceAnnotation
persistent identity: annotation2312564
display id: annotation2312564
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312564/range2312564/1
component: BBa_J61104

https://synbiohub.org/public/igem/BBa_M39412/annotation2312564/range2312564/1
type: Range
persistent identity: range2312564
display id: range2312564
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 869
end: 880
orientation: inline

BBa_K137010
type: SequenceAnnotation
persistent identity: annotation2312567
display id: annotation2312567
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312567/range2312567/1
component: BBa_K137010

https://synbiohub.org/public/igem/BBa_M39412/annotation2312567/range2312567/1
type: Range
persistent identity: range2312567
display id: range2312567
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 889
end: 923
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2312574
display id: annotation2312574
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312574/range2312574/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M39412/annotation2312574/range2312574/1
type: Range
persistent identity: range2312574
display id: range2312574
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 932
end: 1060
orientation: inline

BBa_I14033
type: SequenceAnnotation
persistent identity: annotation2312578
display id: annotation2312578
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312578/range2312578/1
component: BBa_I14033

https://synbiohub.org/public/igem/BBa_M39412/annotation2312578/range2312578/1
type: Range
persistent identity: range2312578
display id: range2312578
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1069
end: 1106
orientation: inline

BBa_K137008
type: SequenceAnnotation
persistent identity: annotation2312581
display id: annotation2312581
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312581/range2312581/1
component: BBa_K137008

https://synbiohub.org/public/igem/BBa_M39412/annotation2312581/range2312581/1
type: Range
persistent identity: range2312581
display id: range2312581
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1115
end: 1149
orientation: inline

BBa_J61103
type: SequenceAnnotation
persistent identity: annotation2312582
display id: annotation2312582
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312582/range2312582/1
component: BBa_J61103

https://synbiohub.org/public/igem/BBa_M39412/annotation2312582/range2312582/1
type: Range
persistent identity: range2312582
display id: range2312582
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1158
end: 1169
orientation: inline

BBa_M0050
type: SequenceAnnotation
persistent identity: annotation2312584
display id: annotation2312584
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312584/range2312584/1
component: BBa_M0050

https://synbiohub.org/public/igem/BBa_M39412/annotation2312584/range2312584/1
type: Range
persistent identity: range2312584
display id: range2312584
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1178
end: 1210
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2312591
display id: annotation2312591
version: 1
top level: BBa_M39412
location: https://synbiohub.org/public/igem/BBa_M39412/annotation2312591/range2312591/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M39412/annotation2312591/range2312591/1
type: Range
persistent identity: range2312591
display id: range2312591
version: 1
top level: BBa_M39412
direction: direction/reverse
start: 1219
end: 1347
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

tetR
type: ComponentDefinition
persistent identity: BBa C0040
display id: BBa_C0040
version: 1
was generated by: iGEM to SBOL conversion
description: tetracycline repressor from transposon Tn10 (+LVA)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Coding region for the TetR protein without the Ribosome Binding Site. Modified with an LVA tail for rapid degradation of the protein and faster fall time for the emission. TetR binds to the pTet regulator (BBa_R0040). aTc (anhydrotetracycline) binds to TetR and inhibits its operation.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 References (unparsed) here: Elowitz, M. B. Transport, Assembly, and Dynamics in Systems of Interacting Proteins. Thesis, Princeton Univ., Princeton (1999). Lutz R, Bujard H., Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements. Nucleic Acids Res. 1997 Mar 15;25(6):1203-10. PMID: 9092630 BBa_C0040 TetR Protein is based on the TetR sequence from Elowitz's repressilator. It has been modified to include a rapid degradation LVA tail, and includes the BioBrick standard assembly head and tail restriction sites. The RBS has been removed. The stop codon has been changed from TAA to a double stop codon TAATAA.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman.
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: tetR
sequence annotation: SsrA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0040_sequence/1

tetR
type: SequenceAnnotation
persistent identity: annotation23329
display id: annotation23329
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0040/annotation23329/range23329/1
type: Range
persistent identity: range23329
display id: range23329
version: 1
top level: tetR
direction: direction/forward
start: 4
end: 620
orientation: inline

SsrA
type: SequenceAnnotation
persistent identity: annotation23330
display id: annotation23330
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
role: feature/tag
role: SO:0000324

https://synbiohub.org/public/igem/BBa_C0040/annotation23330/range23330/1
type: Range
persistent identity: range23330
display id: range23330
version: 1
top level: tetR
direction: direction/forward
start: 621
end: 654
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213989
display id: annotation2213989
version: 1
top level: tetR
location: https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
role: SO:0000807
role: feature/barcode

https://synbiohub.org/public/igem/BBa_C0040/annotation2213989/range2213989/1
type: Range
persistent identity: range2213989
display id: range2213989
version: 1
top level: tetR
direction: direction/forward
start: 661
end: 685
orientation: inline

fimE IRL
type: ComponentDefinition
persistent identity: BBa K137010
display id: BBa_K137010
version: 1
was generated by: iGEM to SBOL conversion
description: fimE IRL
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
mutable description: fimE inverted repeat left recombination site
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Allen Lin
type: DnaRegion
role: SO:0000110
role: partType/DNA
sequence annotation: IRL in
sequence annotation: IRL out
sequence: https://synbiohub.org/public/igem/BBa_K137010_sequence/1

IRL in
type: SequenceAnnotation
persistent identity: annotation1963843
display id: annotation1963843
version: 1
top level: fimE IRL
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137010/annotation1963843/range1963843/1
type: Range
persistent identity: range1963843
display id: range1963843
version: 1
top level: fimE IRL
direction: direction/forward
start: 1
end: 17
orientation: inline

IRL out
type: SequenceAnnotation
persistent identity: annotation1963844
display id: annotation1963844
version: 1
top level: fimE IRL
location: https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137010/annotation1963844/range1963844/1
type: Range
persistent identity: range1963844
display id: range1963844
version: 1
top level: fimE IRL
direction: direction/forward
start: 19
end: 35
orientation: inline

Cat
type: ComponentDefinition
persistent identity: BBa I14033
display id: BBa_I14033
version: 1
was generated by: iGEM to SBOL conversion
description: P(Cat)
created: 2004-08-03T11:00:00Z
modified: 2015-08-31T04:07:37Z
mutable provenance: Plasmid pACYC184
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Constitutive Promoter, Medium Transcription
discontinued: false
dominant: false
experience: experience/None
group u list: _4_
m user id: 0
owner id: 171
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Vikram Vijayan, Allen Hsu, Lawrence Fomundam
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: P(Cat)
sequence annotation: -35
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I14033_sequence/1

P(Cat)
type: SequenceAnnotation
persistent identity: annotation1026174
display id: annotation1026174
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
role: SO:0000057
role: feature/operator

https://synbiohub.org/public/igem/BBa_I14033/annotation1026174/range1026174/1
type: Range
persistent identity: range1026174
display id: range1026174
version: 1
top level: Cat
direction: direction/forward
start: 1
end: 38
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1026172
display id: annotation1026172
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026172/range1026172/1
type: Range
persistent identity: range1026172
display id: range1026172
version: 1
top level: Cat
direction: direction/forward
start: 4
end: 9
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1026173
display id: annotation1026173
version: 1
top level: Cat
location: https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I14033/annotation1026173/range1026173/1
type: Range
persistent identity: range1026173
display id: range1026173
version: 1
top level: Cat
direction: direction/forward
start: 27
end: 32
orientation: inline

BBa_J61105
type: ComponentDefinition
persistent identity: BBa J61105
display id: BBa_J61105
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61105_sequence/1

BBa_J61106
type: ComponentDefinition
persistent identity: BBa J61106
display id: BBa_J61106
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_J61106_sequence/1

BBa_J61104
type: ComponentDefinition
persistent identity: BBa J61104
display id: BBa_J61104
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence: https://synbiohub.org/public/igem/BBa_J61104_sequence/1

LAA
type: ComponentDefinition
persistent identity: BBa M0050
display id: BBa_M0050
version: 1
was generated by: iGEM to SBOL conversion
description: AANDENYALAA. (Very fast) SsrA degradation tag.
created: 2007-12-05T12:00:00Z
modified: 2015-05-08T01:13:51Z
mutable provenance: C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.
owned by: james
owned by: myers
mutable description: This sequence codes for the amino acid sequence AANDENYALAA, which when fused to the C-terminal of proteins, will make the protein susceptible to very fast degradation through SspB-mediated binding to the ClpX protease. The following rates of degradation of this tag are pulled from the corresponding references below: ~5 Vmax/ [Clpx6] min-1 from (1) ~0.5%/min on log scale from (2) ~1 min half life from (3) See the following references for further information on degradation rates and mechanisms of this tag: (1)McGinness, Baker, Sauer. 2006. Mol. Cell. 22:701. (2)Flynn et al 2003. Mol. Cell. 11: 671. Flynn et al. 2001. PNAS 98(19): 10584. Anderson et al 1998. App. Env. Microbiol. 64(6):2240.
discontinued: false
dominant: false
experience: experience/None
group u list: _11_
m user id: 0
owner id: 2398
owning group id: 11
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: C-terminal tag. Degradation rate is very fast. Deviations from this sequence in key amino acids will lower degradation rates (see Parts BBa_M0051, BBa_M0052, BBa_M0053). Three C-terminal aa's (LAA in this case) are necessary and sufficient for ClpX binding and degradation. Upstream aa sequence serves as a binding site for SspB, which guides rapid binding to ClpX.
star: false
creator: Felix Moser
type: DnaRegion
role: SO:0000324
role: partType/Tag
sequence annotation: WT SsrA tag AANDENYALAA
sequence: https://synbiohub.org/public/igem/BBa_M0050_sequence/1

WT SsrA tag AANDENYALAA
type: SequenceAnnotation
persistent identity: annotation1958880
display id: annotation1958880
version: 1
top level: LAA
location: https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_M0050/annotation1958880/range1958880/1
type: Range
persistent identity: range1958880
display id: range1958880
version: 1
top level: LAA
direction: direction/forward
start: 1
end: 33
orientation: inline

IRR
type: ComponentDefinition
persistent identity: BBa K137008
display id: BBa_K137008
version: 1
was generated by: iGEM to SBOL conversion
description: fimE IRR
created: 2008-06-19T11:00:00Z
modified: 2015-05-08T01:10:08Z
mutable provenance: pFIP plasmid
owned by: james
owned by: myers
mutable description: fimE inverted repeat right recombination site
discontinued: false
dominant: false
experience: experience/Issues
group u list: _187_
m user id: 0
owner id: 3112
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Allen Lin
type: DnaRegion
role: partType/DNA
role: SO:0000110
sequence annotation: IRR out
sequence annotation: IRR in
sequence: https://synbiohub.org/public/igem/BBa_K137008_sequence/1

IRR out
type: SequenceAnnotation
persistent identity: annotation1963841
display id: annotation1963841
version: 1
top level: IRR
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137008/annotation1963841/range1963841/1
type: Range
persistent identity: range1963841
display id: range1963841
version: 1
top level: IRR
direction: direction/forward
start: 1
end: 17
orientation: inline

IRR in
type: SequenceAnnotation
persistent identity: annotation1963842
display id: annotation1963842
version: 1
top level: IRR
location: https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_K137008/annotation1963842/range1963842/1
type: Range
persistent identity: range1963842
display id: range1963842
version: 1
top level: IRR
direction: direction/forward
start: 19
end: 35
orientation: inline

BBa_J61103
type: ComponentDefinition
persistent identity: BBa J61103
display id: BBa_J61103
version: 1
was generated by: iGEM to SBOL conversion
description: Ribosome Binding Site Family Member
created: 2007-04-23T11:00:00Z
modified: 2015-08-31T02:03:00Z
mutable provenance: N/A
owned by: james
owned by: myers
mutable description: {{JCA_Arkin_RBSFamily}}
discontinued: false
dominant: false
experience: experience/Works
group u list: _95_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: false
creator: John Anderson
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_J61103_sequence/1

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

https://synbiohub.org/public/igem/BBa_M39412_sequence/1
type: Sequence
persistent identity: BBa M39412 sequence
display id: BBa_M39412_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagaaagataggagatactagagaaagacatgacgtactagagaaagaagggacatactagagtctatgagtcaaaatggccccaattgtcttgtatttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagggcacgtaagaggttccaactttcaccataatgaaacatactagagaagatgaaacatttggggccaaactgtccatattatactagagaaagattagacatactagaggctgctaacgacgaaaactacgctctggctgcttactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0040_sequence/1
type: Sequence
persistent identity: BBa C0040 sequence
display id: BBa_C0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcgaaggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgggctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggcaagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttaggtacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagagaatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgctaaagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaaggtgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtccgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M0050_sequence/1
type: Sequence
persistent identity: BBa M0050 sequence
display id: BBa_M0050_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gctgctaacgacgaaaactacgctctggctgct
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137008_sequence/1
type: Sequence
persistent identity: BBa K137008 sequence
display id: BBa_K137008_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aagatgaaacatttggggccaaactgtccatatta
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K137010_sequence/1
type: Sequence
persistent identity: BBa K137010 sequence
display id: BBa_K137010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tctatgagtcaaaatggccccaattgtcttgtatt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I14033_sequence/1
type: Sequence
persistent identity: BBa I14033 sequence
display id: BBa_I14033_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggcacgtaagaggttccaactttcaccataatgaaaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61105_sequence/1
type: Sequence
persistent identity: BBa J61105 sequence
display id: BBa_J61105_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagacatgacg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61106_sequence/1
type: Sequence
persistent identity: BBa J61106 sequence
display id: BBa_J61106_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagataggaga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61104_sequence/1
type: Sequence
persistent identity: BBa J61104 sequence
display id: BBa_J61104_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaagggaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J61103_sequence/1
type: Sequence
persistent identity: BBa J61103 sequence
display id: BBa_J61103_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagattagaca
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z