LOCUS BBa_M41006 6776 bp DNA linear 15-Nov-2024 DEFINITION Hydrocarbon Sensor ACCESSION BBa_M41006 VERSION BBa_M41006.1 FEATURES Location/Qualifiers promoter 1..35 /label=BBa_J23119 misc_feature 48..51 /label=conserved RBS 44..55 /label=BBa_B0034 CDS 62..1540 /label=BBa_M41000 CDS 1547..2239 /label=BBa_M41001 stem_loop 2259..2302 /label=stem_loop misc_feature 2248..2327 /label=BBa_B0010 terminator 2248..2327 /label=BBa_B0010 stem_loop 2343..2362 /label=T7 TE misc_feature 2369..2369 /label=stop polyA_site 2363..2376 /label=polya misc_feature 2336..2376 /label=BBa_B0012 terminator 2336..2376 /label=BBa_B0012 terminator 2248..2376 /label=BBa_B0015 promoter 2385..2445 /label=BBa_M41002 promoter 2454..2517 /label=BBa_M41003 misc_feature 2530..2533 /label=conserved RBS 2526..2537 /label=BBa_B0034 CDS 2544..5235 /label=BBa_M41004 stem_loop 5255..5298 /label=stem_loop misc_feature 5244..5323 /label=BBa_B0010 terminator 5244..5323 /label=BBa_B0010 stem_loop 5339..5358 /label=T7 TE misc_feature 5365..5365 /label=stop polyA_site 5359..5372 /label=polya misc_feature 5332..5372 /label=BBa_B0012 terminator 5332..5372 /label=BBa_B0012 terminator 5244..5372 /label=BBa_B0015 promoter 5381..5460 /label=BBa_M41005 misc_feature 5473..5476 /label=conserved RBS 5469..5480 /label=BBa_B0034 misc_feature 6576..6614 /label=LVA CDS 5487..6614 /label=lacI-LVA misc_feature 5487..6614 /label=BBa_C0012 misc_feature 6615..6639 /label=Help:Barcodes CDS 5487..6614 /label=BBa_C0012 stem_loop 6659..6702 /label=stem_loop misc_feature 6648..6727 /label=BBa_B0010 terminator 6648..6727 /label=BBa_B0010 stem_loop 6743..6762 /label=T7 TE misc_feature 6769..6769 /label=stop polyA_site 6763..6776 /label=polya misc_feature 6736..6776 /label=BBa_B0012 terminator 6736..6776 /label=BBa_B0012 terminator 6648..6776 /label=BBa_B0015 ORIGIN 1 ttgacagcta gctcagtcct aggtataatg ctagctacta gagaaagagg agaaatacta 61 gatgaactgt tcgctgcggt gtcgattttt tttgattctt gttatggcag gattttcttt 121 ttttgttgcg ctattcggga tgcgattaat gcataagatg gctgaatttg cttattttga 181 aagggagcat gttgttgcct tgagcaaggt gtactacgag ctgcataaaa aagaaataaa 241 tataagtttt atagtagggc aggtgcagag ggctcgtaga caaactacag ctgtaaacag 301 tttgtggaag ggtgataaag ctcttttaag attactggga aagggcttga ttcttgagct 361 gtctgaagct tcagaaataa aacttgggct gctcgagagg tatgcaagta gcatttataa 421 agatggatta aaccctgggc atattgagga aatgaaacga cttgtaagtt ggccctacac 481 gaactctaat cgcttcggga ttgaaattgc agatatttcc aaacgcgtta aagcttacgt 541 gtattttctt gttgtatcaa ttaattgtct cttttttgtt gttatttttc ttcttatgaa 601 aaagactcgc agttcaatag atgaaatagt tcatgtcatg aatgatatgt caagaggtga 661 tcttacatat cgcactatac cgagcaatga cgaagtgggt aaaatgcagt cttcaatcat 721 tgcgatgggg gctggggtaa gcgccttgat tgaatcaata aaacacatac aaggtgatct 781 gtttaattca gcgggcgagg ctttaaatat ttcacaaagc acgtcaaacg atatatgtga 841 ccaagcaggt aagatcgacg aatttgtcag tgcgctatca cagatttctt tcgctataac 901 tgaaacttca aatgcggcaa ataagtcctc agcactatca tctgaggggc ggcagttggc 961 ggttcatggt caaaaagcta ttgaaacagc cgtatcatct ataaatgcct tgtcacaacg 1021 agttaatgat tcacatgtgg ctattaaatg cattgaagct gatattgcaa aaataggtaa 1081 aatcattgaa attattgatc aaatcactga tcaaacaaat ctgctggctt tgaatgccgc 1141 gattgaggct gcacatgcag gcgaggccgg gaagggcttt gctgttgttg ctgatgaagt 1201 tcgaagctta gctcaacgta caaataattc cacttatgaa attcaggcga tgattgcttc 1261 tttaaataaa ggtatatttt ttgctcttgg tgtcatggga gactgtgtcg ttgaatcaaa 1321 aaatagtgtt aatgcagcaa gcgaagctag tagatcaatt gagaagattg tagattcagt 1381 atcccaagtt atgttgcaga ttgcacaagt tgcaactgct tcagaggagc agagtgctgt 1441 tgtaaaagac atgctggata atgcaaatat tattagagaa attgcggcag gagttgagtt 1501 gggtagcagg agaatttcgg aggtaaatac tcacagatag tactagatga gtttttctaa 1561 ttataaagta atcgcgatgc cggtgttggt tgctaatttt gttttggggg cggccactgc 1621 atgggcgaat gaaaattatc cggcgaaatc tgctggctat aatcagggtg actgggtcgc 1681 tagcttcaat ttttctaagg tctatgtggg tgaggagctt ggcgatctaa atgttggagg 1741 gggggctttg ccaaatgctg atgtaagtat tggtaatgat acaacactta cgtttgatat 1801 cgcctatttt gttagctcaa atatagcggt ggattttttt gttggggtgc cagctagggc 1861 taaatttcaa ggtgagaaat caatctcctc gctgggaaga gtcagtgaag ttgattacgg 1921 ccctgcaatt ctttcgcttc aatatcatta cgatagcttt gagcgacttt atccatatgt 1981 tggggttggt gttggtcggg tgctattttt tgataaaacc gacggtgctt tgagttcgtt 2041 tgatattaag gataaatggg cgcctgcttt tcaggttggc cttagatatg accttggtaa 2101 ctcatggatg ctaaattcag atgtgcgtta tattcctttc aaaacggacg tcacaggtac 2161 tcttggcccg gttcctgttt ctactaaaat tgaggttgat cctttcattc tcagtcttgg 2221 tgcgtcatat gttttctaat actagagcca ggcatcaaat aaaacgaaag gctcagtcga 2281 aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac tagagtcaca 2341 ctggctcacc ttcgggtggg cctttctgcg tttatatact agagttcaga ccttccctag 2401 taaatatttt gcaccaccga tcatgccgac tacacttaag tgtagtacta gagttttaat 2461 atttaacacc gtaacctatg gtgaaaattt ccagtcagct ggcgcgagaa tagcatatac 2521 tagagaaaga ggagaaatac tagatgaaaa taataataaa taatgatttc ccggtcgcta 2581 aggtcggagc ggatcaaatt acgactctag taagtgccaa agttcatagt tgcatatatc 2641 ggccaagatt gagtatcgcg gatggagccg ctcccagagt atgcctttac agagccccac 2701 ctggatatgg gaaaaccgtt gctcttgcgt tcgagtggct acgccacaga acagccggac 2761 gtcctgcagt gtggctttct ttaagagcca gttcttacag tgaatttgat atctgcgcag 2821 agattattga gcagcttgaa actttcgaaa tggtaaaatt cagccgtgtg agagagggtg 2881 tgagcaagcc tgcgctcttg cgagaccttg catctagtct ttggcagagc acctcgaata 2941 acgagataga aacgctagtt tgtttggata atattaatca tgacttagac ttgccgttgt 3001 tgcacgcact tatggagttt atgttaaata caccaaaaaa tatcaggttt gcagttgcag 3061 gcaatacaat aaaagggttc tcgcagctta aacttgcagg cgctatgcgg gagtacaccg 3121 agaaagactt ggcctttagc gcagaagagg cggtggcgtt agcggaggca gagtctgttc 3181 ttggagttcc tgaagaacag atagagacct tggtgcaaga agttgagggg tggcctgctc 3241 ttgtagtttt tttgttaaag cgtgagttgc cggccaagca tatttcagca gtagttgaag 3301 tagacaatta ctttagggat gaaatatttg aggcgattcc cgagcgctat cgtgtttttc 3361 ttgcaaattc ttcattgctc gatttcgtga cgcctgatca atacaattat gtattcaaat 3421 gcgtcaatgg ggtctcatgt attaagtatt taagcactaa ttacatgttg cttcgccatg 3481 tgagcggtga gccagcgcag tttacactgc atccagtact gcgtaatttt ctacgagaaa 3541 ttacttggac tgaaaatcct gctaaaagat cctacctgct taagcgtgca gctttctggc 3601 attggcgtag aggtgaatac cagtatgcaa tacgaatatc cctacgggcg aatgactgtc 3661 gctgggcagt cagcatgtct gagagaataa ttttagattt gtcatttcgt cagggcgaaa 3721 tagatgcgct gagacagtgg ctgttagagc tgccgaagca ggcctggcac caaaaaccca 3781 tagtgcttat tagttacgcg tgggtattgt atttcagtca gcaaggcgcg cgagcagaga 3841 agttaattaa agacctatct tcacaatccg ataaaaaaaa taaatggcaa gaaaaggaat 3901 ggctgcagct tgtgcttgca ataggtaaag caaccaaaga tgaaatgctt tcgagtgagg 3961 agctctgtaa taagtggatt agtttatttg gggattcaaa cgcagttgga aaaggggccg 4021 cgctaacctg tttggctttt atttttgcca gtgagtatag atttgcagag ttggagaagg 4081 tgctggctca ggcccaagcc gtgaataaat ttgcaaaaca aaattttgct tttggttggc 4141 tgtatgtcgc gaggtttcaa caagccctag caagcggaaa aatgggctgg gcgaggcaga 4201 ttataactca agcacgcaca gacagtcgcg cgcagatgat ggaatccgag tttacttcga 4261 aaatgtttga cgctctagag cttgagttac attatgaatt gcgctgcttg gacacctcag 4321 aagaaaagct ctccaaaatt ttagagttca tttccaatca cggggtgaca gacgtgtttt 4381 tttccgtatg ccgtgctgtg tcagcttggc ggcttggaag gagtgaccta aatggctcca 4441 ttgagatatt ggagtgggcg aaggcgcatg cggttgaaaa aaatctacca agattggaag 4501 ttatgagcca aattgagatc tatcagcgct tagtctgtca aggcataacg ggcataaata 4561 atttaaaaac tcttgaagat cataagattt tctccggaca gcactcagcc cccctaaaag 4621 cacgcctgct gcttgttcaa tcactagtgc tttcccgaga tcggaacttt catagtgccg 4681 cgcacagagc gttattggct attcagcaag cccgtaaaat taacgcgggc cagctggaag 4741 tccgtggatt attgtgtttg gccggagcgc aggcaggtgc cggtgattta aaaaaggctc 4801 agcttaacat tgtttatgca gtggagatag caaaacagct tcaatgcttt caaacagttc 4861 ttgatgaagt atgtttaatt gagcgaataa taccggcttc atgtgaagcc ttcacagcag 4921 ttaatttaga tcaagcgatt ggggctttta gtcttccgcg aatagttgag attggaaagt 4981 ccgcagagaa taaagctgac gctttattga cacggaagca gattgctgtc ttgaggcttg 5041 taaaagaggg gtgctcaaac aaacaaatag caacaaatat gcatgtcacc gaagatgcta 5101 taaagtggca tatgaggaaa atatttgcca ccttgaatgt agtgaatcgc acgcaagcaa 5161 caattgaagc tgagcgtcaa ggaattatct aaaataatcg gcattaagtg atatagtgaa 5221 aagtataccg gagagtacta gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga 5281 ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga gtcacactgg 5341 ctcaccttcg ggtgggcctt tctgcgttta tatactagag cgtgtttttc cagcagacga 5401 cggagcaaaa actacccgta ggtgtagttg gcgcaagcgt ccgattagct caggtttaag 5461 tactagagaa agaggagaaa tactagatgg tgaatgtgaa accagtaacg ttatacgatg 5521 tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg cgtggtgaac caggccagcc 5581 acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat ggcggagctg aattacattc 5641 ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt gctgattggc gttgccacct 5701 ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc gattaaatct cgcgccgatc 5761 aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag cggcgtcgaa gcctgtaaag 5821 cggcggtgca caatcttctc gcgcaacgcg tcagtgggct gatcattaac tatccgctgg 5881 atgaccagga tgccattgct gtggaagctg cctgcactaa tgttccggcg ttatttcttg 5941 atgtctctga ccagacaccc atcaacagta ttattttctc ccatgaagac ggtacgcgac 6001 tgggcgtgga gcatctggtc gcattgggtc accagcaaat cgcgctgtta gcgggcccat 6061 taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca taaatatctc actcgcaatc 6121 aaattcagcc gatagcggaa cgggaaggcg actggagtgc catgtccggt tttcaacaaa 6181 ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat gctggttgcc aacgatcaga 6241 tggcgctggg cgcaatgcgc gccattaccg agtccgggct gcgcgttggt gcggatatct 6301 cggtagtggg atacgacgat accgaagaca gctcatgtta tatcccgccg ttaaccacca 6361 tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga ccgcttgctg caactctctc 6421 agggccaggc ggtgaagggc aatcagctgt tgcccgtctc actggtgaaa agaaaaacca 6481 ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc 6541 tggcacgaca ggtttcccga ctggaaagcg ggcaggctgc aaacgacgaa aactacgctt 6601 tagtagctta ataactctga tagtgctagt gtagatctct actagagcca ggcatcaaat 6661 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa 6721 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata //