BBa_M41002 1 BBa_M41002 pAlkS1 promoter in Pseudomonas putida 2013-05-11T11:00:00Z 2015-05-08T01:14:08Z Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9 It is a first promoter of AlkS gene which is recognized by &#963;S-RNA polymerase. It is downregulated by AlkS protein in the absence of alkanes, keeping the level of AlkS constant in the cell. When there are alkanes present in the cell, AlkS-hydrocarbon complex represses pAlkS1 and activates second promoter, pAlkS2, to produce more AlkS. false false _1503_ 0 16820 9 Not in stock false We cannot separate pAlkS1, pAlkS2 and AlkS gene because of self-regulatory system. false Hao Xing BBa_M41005 1 BBa_M41005 pAlkB promoter in Pseudomonas putida 2013-05-11T11:00:00Z 2015-05-08T01:14:08Z Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. It is an inducible promoter for alkBFGHJKL operon (alkane degradation operon). It is induced by AlkS-Hydrocarbon complex. The operon encodes different enzymes responsible for alkane degradation. Expression of the PalkB promoter is also modulated by catabolite repression depending on the carbon source being used. false false _1503_ 0 16820 9 Not in stock false pAlkB is regulated by different carbon sources. When alkane is the only major source for energy in the cell, pAlkB will express the operon. false Hao Xing BBa_M41003 1 BBa_M41003 pAlkS2 promoter in Pseudomonas putida 2013-05-11T11:00:00Z 2015-05-08T01:14:08Z Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. It is a second promoter of AlkS gene. It is upregulated by AlkS-hydrocarbon complex formed in the presence of alkanes in the cell. false false _1503_ 0 16820 9 Not in stock false We cannot separate pAlkS1, pAlkS2 and AlkS gene because of self-regulatory system. false Hao Xing BBa_M41004 1 BBa_M41004 AlkS coding sequence in Pseudomonas putida 2013-05-11T11:00:00Z 2015-05-08T01:14:08Z Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. This gene encodes a transcription factor AlkS, which activates alkane degradation operon. AlkS self-regulates its expression. In the presence of alkanes, AlkS forms AlkS-hydrocarbon (AlkS-HC) complex which downregulates pAlkS1 and upregulates pAlkS2. AlkS-HC complex activates pAlkB which is responsible for expression of alkane degradation operon. false false _1503_ 0 16820 9 Not in stock false We cannot separate pAlkS1, pAlkS2 and AlkS gene because of self-regulatory system. false Hao Xing BBa_C0012 1 lacI lacI repressor from E. coli (+LVA) 2003-01-31T12:00:00Z 2015-08-31T04:07:23Z represillator of Elowitz and Leibler (2000) Released HQ 2013 Coding region for the LacI protein with an LVA degradation tail and without an RBS. LacI binds to the pLac regulator <bb_part>BBa_R0010</bb_part> and PLlac01 hybrid regulator <bb_part>BBa_R0011</bb_part> and inhibits transcription. IPTG (Isopropylthiogalactoside) binds to LacI and inhibits its operation, therefore promoting transcription.</P> <P>A rapid degredation tail (LVA) has been added to improve the High to Low performance of this part.</P> false false _1_ 0 24 7 In stock false References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P> References (unparsed) here: <p>Elowitz, M.B., Leibler, S. A synthetic oscillatory network of transcriptional regulators. <em>Nature</em> 403, 335-338 (2000). <a href="http://biobricks.ai.mit.edu/BB_References.htm#ELOW00">[ELOW00]</a><br> <br> </P> <P>Sequence taken from the repressilator of Elowitz and Leibler (2000). The obtained sequence was compared to the wild-type sequence for LacI obtained through a database search. The sequence had been modified from the wild-type in that wild-type GTG start was changed to an ATG start (note, actual ORF in E.coli has several GTG starts it would seem). The LVA tag has been added for quicker degradation.<P> Incompatible with systems containing LacI, lactose, or IPTG. true Grace Kenney, Daniel Shen, Neelaksh Varshney, Samantha Sutton annotation2213988 1 Help:Barcodes range2213988 1 1129 1153 annotation1722 1 LVA range1722 1 1090 1128 annotation7031 1 BBa_C0012 range7031 1 1 1128 annotation1723 1 lacI-LVA range1723 1 1 1128 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_M41006 1 BBa_M41006 Hydrocarbon Sensor 2013-05-11T11:00:00Z 2015-05-08T01:14:08Z Canosa, I., et al. "A Positive Feedback Mechanism Controls Expression of AlkS, the Transcriptional Regulator of the Pseudomonas Oleovorans Alkane Degradation Pathway." Molecular microbiology 35.4 (2000): 791-9. Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. This is a sequence for hydrocarbon sensor. The input for this sensor is n-alkane chain and output is LacI. It has a sequence which encodes membrane protein (AlkL) and chemotaxis protein (AlkN) responsible for hydrocarbon sensing. This sequence does not code for any major alkane degrading enzymes except AlkL and AlkN. false false _1503_ 0 16820 9 Not in stock false This sensor is specific for our system. The output can be changed by removing LacI gene and adding other gene. false Hao Xing component2236877 1 BBa_B0034 component2236866 1 BBa_M41001 component2236874 1 BBa_M41002 component2236899 1 BBa_B0015 component2236890 1 BBa_C0012 component2236886 1 BBa_M41005 component2236865 1 BBa_M41000 component2236875 1 BBa_M41003 component2236864 1 BBa_B0034 component2236862 1 BBa_J23119 component2236878 1 BBa_M41004 component2236885 1 BBa_B0015 component2236888 1 BBa_B0034 component2236873 1 BBa_B0015 annotation2236875 1 BBa_M41003 range2236875 1 2454 2517 annotation2236886 1 BBa_M41005 range2236886 1 5381 5460 annotation2236865 1 BBa_M41000 range2236865 1 62 1540 annotation2236878 1 BBa_M41004 range2236878 1 2544 5235 annotation2236864 1 BBa_B0034 range2236864 1 44 55 annotation2236899 1 BBa_B0015 range2236899 1 6648 6776 annotation2236885 1 BBa_B0015 range2236885 1 5244 5372 annotation2236890 1 BBa_C0012 range2236890 1 5487 6614 annotation2236873 1 BBa_B0015 range2236873 1 2248 2376 annotation2236862 1 BBa_J23119 range2236862 1 1 35 annotation2236888 1 BBa_B0034 range2236888 1 5469 5480 annotation2236866 1 BBa_M41001 range2236866 1 1547 2239 annotation2236877 1 BBa_B0034 range2236877 1 2526 2537 annotation2236874 1 BBa_M41002 range2236874 1 2385 2445 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M41001 1 BBa_M41001 AlkL coding sequence in Pseudomonas putida 2013-05-10T11:00:00Z 2015-05-08T01:14:08Z Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. This gene encodes a membrane protein which probably facilitates uptake of hydrocarbons. false false _1503_ 0 16820 9 Not in stock false The function of this protein is still unknown. We have to experimentally determine the function using alkBFGHJKL operon. false Hao Xing BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1686 1 T7 TE range1686 1 8 27 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_J23119 1 BBa_J23119 constitutive promoter family member 2006-08-23T11:00:00Z 2015-08-31T04:08:40Z Overlap extension of synthetic oligonucleotides Released HQ 2013 Later false true _52_ 0 483 95 In stock false N/A true John Anderson BBa_M41000 1 BBa_M41000 AlkN coding sequence in Pseudomonas putida 2013-05-10T11:00:00Z 2015-05-08T01:14:08Z Van Beilen, JB, et al. "Analysis of Pseudomonas Putida Alkane-Degradation Gene Clusters and Flanking Insertion Sequences: Evolution and Regulation of the Alk Genes." Microbiology 147.6 (2001): 1621-30. This gene encodes a methyl-accepting transducer protein that may be involved in chemotaxis to alkanes. false false _1503_ 0 16820 9 Not in stock false The original AlkN has promoter and ribosome binding site. We have only taken coding region for our system. false Hao Xing BBa_M41001_sequence 1 atgagtttttctaattataaagtaatcgcgatgccggtgttggttgctaattttgttttgggggcggccactgcatgggcgaatgaaaattatccggcgaaatctgctggctataatcagggtgactgggtcgctagcttcaatttttctaaggtctatgtgggtgaggagcttggcgatctaaatgttggagggggggctttgccaaatgctgatgtaagtattggtaatgatacaacacttacgtttgatatcgcctattttgttagctcaaatatagcggtggatttttttgttggggtgccagctagggctaaatttcaaggtgagaaatcaatctcctcgctgggaagagtcagtgaagttgattacggccctgcaattctttcgcttcaatatcattacgatagctttgagcgactttatccatatgttggggttggtgttggtcgggtgctattttttgataaaaccgacggtgctttgagttcgtttgatattaaggataaatgggcgcctgcttttcaggttggccttagatatgaccttggtaactcatggatgctaaattcagatgtgcgttatattcctttcaaaacggacgtcacaggtactcttggcccggttcctgtttctactaaaattgaggttgatcctttcattctcagtcttggtgcgtcatatgttttctaa BBa_B0034_sequence 1 aaagaggagaaa BBa_M41004_sequence 1 atgaaaataataataaataatgatttcccggtcgctaaggtcggagcggatcaaattacgactctagtaagtgccaaagttcatagttgcatatatcggccaagattgagtatcgcggatggagccgctcccagagtatgcctttacagagccccacctggatatgggaaaaccgttgctcttgcgttcgagtggctacgccacagaacagccggacgtcctgcagtgtggctttctttaagagccagttcttacagtgaatttgatatctgcgcagagattattgagcagcttgaaactttcgaaatggtaaaattcagccgtgtgagagagggtgtgagcaagcctgcgctcttgcgagaccttgcatctagtctttggcagagcacctcgaataacgagatagaaacgctagtttgtttggataatattaatcatgacttagacttgccgttgttgcacgcacttatggagtttatgttaaatacaccaaaaaatatcaggtttgcagttgcaggcaatacaataaaagggttctcgcagcttaaacttgcaggcgctatgcgggagtacaccgagaaagacttggcctttagcgcagaagaggcggtggcgttagcggaggcagagtctgttcttggagttcctgaagaacagatagagaccttggtgcaagaagttgaggggtggcctgctcttgtagtttttttgttaaagcgtgagttgccggccaagcatatttcagcagtagttgaagtagacaattactttagggatgaaatatttgaggcgattcccgagcgctatcgtgtttttcttgcaaattcttcattgctcgatttcgtgacgcctgatcaatacaattatgtattcaaatgcgtcaatggggtctcatgtattaagtatttaagcactaattacatgttgcttcgccatgtgagcggtgagccagcgcagtttacactgcatccagtactgcgtaattttctacgagaaattacttggactgaaaatcctgctaaaagatcctacctgcttaagcgtgcagctttctggcattggcgtagaggtgaataccagtatgcaatacgaatatccctacgggcgaatgactgtcgctgggcagtcagcatgtctgagagaataattttagatttgtcatttcgtcagggcgaaatagatgcgctgagacagtggctgttagagctgccgaagcaggcctggcaccaaaaacccatagtgcttattagttacgcgtgggtattgtatttcagtcagcaaggcgcgcgagcagagaagttaattaaagacctatcttcacaatccgataaaaaaaataaatggcaagaaaaggaatggctgcagcttgtgcttgcaataggtaaagcaaccaaagatgaaatgctttcgagtgaggagctctgtaataagtggattagtttatttggggattcaaacgcagttggaaaaggggccgcgctaacctgtttggcttttatttttgccagtgagtatagatttgcagagttggagaaggtgctggctcaggcccaagccgtgaataaatttgcaaaacaaaattttgcttttggttggctgtatgtcgcgaggtttcaacaagccctagcaagcggaaaaatgggctgggcgaggcagattataactcaagcacgcacagacagtcgcgcgcagatgatggaatccgagtttacttcgaaaatgtttgacgctctagagcttgagttacattatgaattgcgctgcttggacacctcagaagaaaagctctccaaaattttagagttcatttccaatcacggggtgacagacgtgtttttttccgtatgccgtgctgtgtcagcttggcggcttggaaggagtgacctaaatggctccattgagatattggagtgggcgaaggcgcatgcggttgaaaaaaatctaccaagattggaagttatgagccaaattgagatctatcagcgcttagtctgtcaaggcataacgggcataaataatttaaaaactcttgaagatcataagattttctccggacagcactcagcccccctaaaagcacgcctgctgcttgttcaatcactagtgctttcccgagatcggaactttcatagtgccgcgcacagagcgttattggctattcagcaagcccgtaaaattaacgcgggccagctggaagtccgtggattattgtgtttggccggagcgcaggcaggtgccggtgatttaaaaaaggctcagcttaacattgtttatgcagtggagatagcaaaacagcttcaatgctttcaaacagttcttgatgaagtatgtttaattgagcgaataataccggcttcatgtgaagccttcacagcagttaatttagatcaagcgattggggcttttagtcttccgcgaatagttgagattggaaagtccgcagagaataaagctgacgctttattgacacggaagcagattgctgtcttgaggcttgtaaaagaggggtgctcaaacaaacaaatagcaacaaatatgcatgtcaccgaagatgctataaagtggcatatgaggaaaatatttgccaccttgaatgtagtgaatcgcacgcaagcaacaattgaagctgagcgtcaaggaattatctaaaataatcggcattaagtgatatagtgaaaagtataccggagag BBa_M41000_sequence 1 atgaactgttcgctgcggtgtcgattttttttgattcttgttatggcaggattttctttttttgttgcgctattcgggatgcgattaatgcataagatggctgaatttgcttattttgaaagggagcatgttgttgccttgagcaaggtgtactacgagctgcataaaaaagaaataaatataagttttatagtagggcaggtgcagagggctcgtagacaaactacagctgtaaacagtttgtggaagggtgataaagctcttttaagattactgggaaagggcttgattcttgagctgtctgaagcttcagaaataaaacttgggctgctcgagaggtatgcaagtagcatttataaagatggattaaaccctgggcatattgaggaaatgaaacgacttgtaagttggccctacacgaactctaatcgcttcgggattgaaattgcagatatttccaaacgcgttaaagcttacgtgtattttcttgttgtatcaattaattgtctcttttttgttgttatttttcttcttatgaaaaagactcgcagttcaatagatgaaatagttcatgtcatgaatgatatgtcaagaggtgatcttacatatcgcactataccgagcaatgacgaagtgggtaaaatgcagtcttcaatcattgcgatgggggctggggtaagcgccttgattgaatcaataaaacacatacaaggtgatctgtttaattcagcgggcgaggctttaaatatttcacaaagcacgtcaaacgatatatgtgaccaagcaggtaagatcgacgaatttgtcagtgcgctatcacagatttctttcgctataactgaaacttcaaatgcggcaaataagtcctcagcactatcatctgaggggcggcagttggcggttcatggtcaaaaagctattgaaacagccgtatcatctataaatgccttgtcacaacgagttaatgattcacatgtggctattaaatgcattgaagctgatattgcaaaaataggtaaaatcattgaaattattgatcaaatcactgatcaaacaaatctgctggctttgaatgccgcgattgaggctgcacatgcaggcgaggccgggaagggctttgctgttgttgctgatgaagttcgaagcttagctcaacgtacaaataattccacttatgaaattcaggcgatgattgcttctttaaataaaggtatattttttgctcttggtgtcatgggagactgtgtcgttgaatcaaaaaatagtgttaatgcagcaagcgaagctagtagatcaattgagaagattgtagattcagtatcccaagttatgttgcagattgcacaagttgcaactgcttcagaggagcagagtgctgttgtaaaagacatgctggataatgcaaatattattagagaaattgcggcaggagttgagttgggtagcaggagaatttcggaggtaaatactcacagatag BBa_J23119_sequence 1 ttgacagctagctcagtcctaggtataatgctagc BBa_M41002_sequence 1 ttcagaccttccctagtaaatattttgcaccaccgatcatgccgactacacttaagtgtag BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M41003_sequence 1 ttttaatatttaacaccgtaacctatggtgaaaatttccagtcagctggcgcgagaatagcata BBa_M41005_sequence 1 cgtgtttttccagcagacgacggagcaaaaactacccgtaggtgtagttggcgcaagcgtccgattagctcaggtttaag BBa_M41006_sequence 1 ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgaactgttcgctgcggtgtcgattttttttgattcttgttatggcaggattttctttttttgttgcgctattcgggatgcgattaatgcataagatggctgaatttgcttattttgaaagggagcatgttgttgccttgagcaaggtgtactacgagctgcataaaaaagaaataaatataagttttatagtagggcaggtgcagagggctcgtagacaaactacagctgtaaacagtttgtggaagggtgataaagctcttttaagattactgggaaagggcttgattcttgagctgtctgaagcttcagaaataaaacttgggctgctcgagaggtatgcaagtagcatttataaagatggattaaaccctgggcatattgaggaaatgaaacgacttgtaagttggccctacacgaactctaatcgcttcgggattgaaattgcagatatttccaaacgcgttaaagcttacgtgtattttcttgttgtatcaattaattgtctcttttttgttgttatttttcttcttatgaaaaagactcgcagttcaatagatgaaatagttcatgtcatgaatgatatgtcaagaggtgatcttacatatcgcactataccgagcaatgacgaagtgggtaaaatgcagtcttcaatcattgcgatgggggctggggtaagcgccttgattgaatcaataaaacacatacaaggtgatctgtttaattcagcgggcgaggctttaaatatttcacaaagcacgtcaaacgatatatgtgaccaagcaggtaagatcgacgaatttgtcagtgcgctatcacagatttctttcgctataactgaaacttcaaatgcggcaaataagtcctcagcactatcatctgaggggcggcagttggcggttcatggtcaaaaagctattgaaacagccgtatcatctataaatgccttgtcacaacgagttaatgattcacatgtggctattaaatgcattgaagctgatattgcaaaaataggtaaaatcattgaaattattgatcaaatcactgatcaaacaaatctgctggctttgaatgccgcgattgaggctgcacatgcaggcgaggccgggaagggctttgctgttgttgctgatgaagttcgaagcttagctcaacgtacaaataattccacttatgaaattcaggcgatgattgcttctttaaataaaggtatattttttgctcttggtgtcatgggagactgtgtcgttgaatcaaaaaatagtgttaatgcagcaagcgaagctagtagatcaattgagaagattgtagattcagtatcccaagttatgttgcagattgcacaagttgcaactgcttcagaggagcagagtgctgttgtaaaagacatgctggataatgcaaatattattagagaaattgcggcaggagttgagttgggtagcaggagaatttcggaggtaaatactcacagatagtactagatgagtttttctaattataaagtaatcgcgatgccggtgttggttgctaattttgttttgggggcggccactgcatgggcgaatgaaaattatccggcgaaatctgctggctataatcagggtgactgggtcgctagcttcaatttttctaaggtctatgtgggtgaggagcttggcgatctaaatgttggagggggggctttgccaaatgctgatgtaagtattggtaatgatacaacacttacgtttgatatcgcctattttgttagctcaaatatagcggtggatttttttgttggggtgccagctagggctaaatttcaaggtgagaaatcaatctcctcgctgggaagagtcagtgaagttgattacggccctgcaattctttcgcttcaatatcattacgatagctttgagcgactttatccatatgttggggttggtgttggtcgggtgctattttttgataaaaccgacggtgctttgagttcgtttgatattaaggataaatgggcgcctgcttttcaggttggccttagatatgaccttggtaactcatggatgctaaattcagatgtgcgttatattcctttcaaaacggacgtcacaggtactcttggcccggttcctgtttctactaaaattgaggttgatcctttcattctcagtcttggtgcgtcatatgttttctaatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttcagaccttccctagtaaatattttgcaccaccgatcatgccgactacacttaagtgtagtactagagttttaatatttaacaccgtaacctatggtgaaaatttccagtcagctggcgcgagaatagcatatactagagaaagaggagaaatactagatgaaaataataataaataatgatttcccggtcgctaaggtcggagcggatcaaattacgactctagtaagtgccaaagttcatagttgcatatatcggccaagattgagtatcgcggatggagccgctcccagagtatgcctttacagagccccacctggatatgggaaaaccgttgctcttgcgttcgagtggctacgccacagaacagccggacgtcctgcagtgtggctttctttaagagccagttcttacagtgaatttgatatctgcgcagagattattgagcagcttgaaactttcgaaatggtaaaattcagccgtgtgagagagggtgtgagcaagcctgcgctcttgcgagaccttgcatctagtctttggcagagcacctcgaataacgagatagaaacgctagtttgtttggataatattaatcatgacttagacttgccgttgttgcacgcacttatggagtttatgttaaatacaccaaaaaatatcaggtttgcagttgcaggcaatacaataaaagggttctcgcagcttaaacttgcaggcgctatgcgggagtacaccgagaaagacttggcctttagcgcagaagaggcggtggcgttagcggaggcagagtctgttcttggagttcctgaagaacagatagagaccttggtgcaagaagttgaggggtggcctgctcttgtagtttttttgttaaagcgtgagttgccggccaagcatatttcagcagtagttgaagtagacaattactttagggatgaaatatttgaggcgattcccgagcgctatcgtgtttttcttgcaaattcttcattgctcgatttcgtgacgcctgatcaatacaattatgtattcaaatgcgtcaatggggtctcatgtattaagtatttaagcactaattacatgttgcttcgccatgtgagcggtgagccagcgcagtttacactgcatccagtactgcgtaattttctacgagaaattacttggactgaaaatcctgctaaaagatcctacctgcttaagcgtgcagctttctggcattggcgtagaggtgaataccagtatgcaatacgaatatccctacgggcgaatgactgtcgctgggcagtcagcatgtctgagagaataattttagatttgtcatttcgtcagggcgaaatagatgcgctgagacagtggctgttagagctgccgaagcaggcctggcaccaaaaacccatagtgcttattagttacgcgtgggtattgtatttcagtcagcaaggcgcgcgagcagagaagttaattaaagacctatcttcacaatccgataaaaaaaataaatggcaagaaaaggaatggctgcagcttgtgcttgcaataggtaaagcaaccaaagatgaaatgctttcgagtgaggagctctgtaataagtggattagtttatttggggattcaaacgcagttggaaaaggggccgcgctaacctgtttggcttttatttttgccagtgagtatagatttgcagagttggagaaggtgctggctcaggcccaagccgtgaataaatttgcaaaacaaaattttgcttttggttggctgtatgtcgcgaggtttcaacaagccctagcaagcggaaaaatgggctgggcgaggcagattataactcaagcacgcacagacagtcgcgcgcagatgatggaatccgagtttacttcgaaaatgtttgacgctctagagcttgagttacattatgaattgcgctgcttggacacctcagaagaaaagctctccaaaattttagagttcatttccaatcacggggtgacagacgtgtttttttccgtatgccgtgctgtgtcagcttggcggcttggaaggagtgacctaaatggctccattgagatattggagtgggcgaaggcgcatgcggttgaaaaaaatctaccaagattggaagttatgagccaaattgagatctatcagcgcttagtctgtcaaggcataacgggcataaataatttaaaaactcttgaagatcataagattttctccggacagcactcagcccccctaaaagcacgcctgctgcttgttcaatcactagtgctttcccgagatcggaactttcatagtgccgcgcacagagcgttattggctattcagcaagcccgtaaaattaacgcgggccagctggaagtccgtggattattgtgtttggccggagcgcaggcaggtgccggtgatttaaaaaaggctcagcttaacattgtttatgcagtggagatagcaaaacagcttcaatgctttcaaacagttcttgatgaagtatgtttaattgagcgaataataccggcttcatgtgaagccttcacagcagttaatttagatcaagcgattggggcttttagtcttccgcgaatagttgagattggaaagtccgcagagaataaagctgacgctttattgacacggaagcagattgctgtcttgaggcttgtaaaagaggggtgctcaaacaaacaaatagcaacaaatatgcatgtcaccgaagatgctataaagtggcatatgaggaaaatatttgccaccttgaatgtagtgaatcgcacgcaagcaacaattgaagctgagcgtcaaggaattatctaaaataatcggcattaagtgatatagtgaaaagtataccggagagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagcgtgtttttccagcagacgacggagcaaaaactacccgtaggtgtagttggcgcaagcgtccgattagctcaggtttaagtactagagaaagaggagaaatactagatggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_C0012_sequence 1 atggtgaatgtgaaaccagtaacgttatacgatgtcgcagagtatgccggtgtctcttatcagaccgtttcccgcgtggtgaaccaggccagccacgtttctgcgaaaacgcgggaaaaagtggaagcggcgatggcggagctgaattacattcccaaccgcgtggcacaacaactggcgggcaaacagtcgttgctgattggcgttgccacctccagtctggccctgcacgcgccgtcgcaaattgtcgcggcgattaaatctcgcgccgatcaactgggtgccagcgtggtggtgtcgatggtagaacgaagcggcgtcgaagcctgtaaagcggcggtgcacaatcttctcgcgcaacgcgtcagtgggctgatcattaactatccgctggatgaccaggatgccattgctgtggaagctgcctgcactaatgttccggcgttatttcttgatgtctctgaccagacacccatcaacagtattattttctcccatgaagacggtacgcgactgggcgtggagcatctggtcgcattgggtcaccagcaaatcgcgctgttagcgggcccattaagttctgtctcggcgcgtctgcgtctggctggctggcataaatatctcactcgcaatcaaattcagccgatagcggaacgggaaggcgactggagtgccatgtccggttttcaacaaaccatgcaaatgctgaatgagggcatcgttcccactgcgatgctggttgccaacgatcagatggcgctgggcgcaatgcgcgccattaccgagtccgggctgcgcgttggtgcggatatctcggtagtgggatacgacgataccgaagacagctcatgttatatcccgccgttaaccaccatcaaacaggattttcgcctgctggggcaaaccagcgtggaccgcttgctgcaactctctcagggccaggcggtgaagggcaatcagctgttgcccgtctcactggtgaaaagaaaaaccaccctggcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcaggctgcaaacgacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z