Hairpin designed to silence canine F1 allergen
This is a composite part that consists of a T7 promoter, the sense sequence for the dog F1 allergen, a hairpin loop, the antisense for the dog F1 allergen and a T1 terminator.
This sequence is structured to form a hairpin loop of double-stranded RNA that will induce the silencing of genes that produce the canine F1 allergen.
The sense and antisense portions must properly form a loop structure in order to induce gene silencing.
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Transcription terminator for the E.coli RNA polymerase.
Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
Transcriptional terminator consisting of a 64 bp stem-loop.
T7 promoter (strong promoter from T7 bacteriophage)
T7 promoter is very specific promoter which is transcribed only by specific T7 RNA polymerase. Usually this promoter is used in expression systems where T7 promoter is cotransfected with T7 RNA polymerase. That ensures strong transcription of desired genes.
This part is simply a string of nucleotides that has been shown to adequately form a loop structure when placed between sense and antisense portions of DNA.
The hairpin loop is a small set of nucleotides that acts as a turning point of the U shape in a DNA hairpin structure.
The structure of a hairpin loop needed to adequately form a U shape to allow bonding of the two strands of DNA.
This enhancer is found in bacteriophages.
This is a simple enhancer for the T7 promoter.
No design considerations had to be taken into account for this part.
This comes from a protein sequence that has been converted into a DNA sequence for the purpose of gene silencing.
This is the primary F1 allergen produced by dogs.
No design considerations had to be made in producing this part.
This comes from the dog F1 allergen which is present in canine dander.
This part is the anti-sense component necessary to form the hairpin structure based off of the common dog F1 allergen.
No design considerations had to be made.
Modified hly for listeriolysin O production
From the bacterium Listeria monocytogenes.
This is a codon-optimized version of hly, a gene that codes for listeriolysin O. Listeriolysin O permits the movement of genetic information to escape through entry vesicles.
This part is optimized for expression in E. coli.
Conversion of the iGEM parts registry to SBOL2.1