LOCUS       BBa_M45101              1484 bp    DNA     linear       07-May-2024
DEFINITION  Composite biobrick intended to induce biofilm formation in the 
            presence of lactose. Made from parts
ACCESSION   BBa_M45101
VERSION     BBa_M45101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=SacI
     misc_feature    248..285
                     /label=CAP binding site
     misc_feature    332..352
                     /label=LacI binding site
     RBS             359..362
                     /label=rbs
     CDS             370..600
                     /label=lacZ'
     promoter        320..325
                     /label=-10
     promoter        297..302
                     /label=-35
     misc_feature    1..600
                     /label=BBa_J33207
     promoter        609..1347
                     /label=BBa_K342003
     stem_loop       1367..1410
                     /label=stem_loop
     misc_feature    1356..1435
                     /label=BBa_B0010
     terminator      1356..1435
                     /label=BBa_B0010
     stem_loop       1451..1470
                     /label=T7 TE
     misc_feature    1477..1477
                     /label=stop
     polyA_site      1471..1484
                     /label=polya
     misc_feature    1444..1484
                     /label=BBa_B0012
     terminator      1444..1484
                     /label=BBa_B0012
     terminator      1356..1484
                     /label=BBa_B0015
ORIGIN
        1 ctcatgttat atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac
       61 cagcgtggac cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt
      121 gcccgtctca ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc
      181 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
      241 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
      301 actttatgct tccggctcgt atgttgtgtg aaattgtgag cggataacaa tttcacacag
      361 gaaacagcta tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg
      421 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
      481 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
      541 gaatggcgct ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtga
      601 tactagagag gaggtatata atgcaagaga actacaagat tctggtggtc gatgacgaca
      661 tgcgcctgcg tgcgctgctg gaacgttatc tcaccgaaca aggcttccag gttcgaagcg
      721 tcgctaatgc agaacagatg gatcgccggc tgactcgtga atctttccat cttatggtac
      781 tggatttaat gttacctggt gaagatggct tgtcgatttg ccgacgtctt cgtagtcaga
      841 gcaacccgat gccgatcatt atggtgacgg cgaaagggga agaagtggac cgtatcgtag
      901 gcctggagat tggcgctgac gactacattc caaaaccgtt taacccgcgt gaactgctgg
      961 cccgtatccg tgcggtgctg cgtcgtcagg cgaacgaact gccaggcgca ccgtcacagg
     1021 aagaggcggt aattgctttc ggtaagttca aacttaacct cggtacgcgc gaaatgttcc
     1081 gcgaagacga gccgatgccg ctcaccagcg gtgagtttgc ggtactgaag gcactggtca
     1141 gccatccgcg tgagccgctc tcccgcgata agctgatgaa ccttgcccgt ggtcgtgaat
     1201 attccgcaat ggaacgctcc atcgacgtgc agatttcgcg tctgcgccgc atggtggaag
     1261 aagatccagc gcatccgcgt tacattcaga ccgtctgggg tctgggctac gtctttgtac
     1321 cggacggctc taaagcatga taataattac tagagccagg catcaaataa aacgaaaggc
     1381 tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg ctctctacta
     1441 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata
//