LOCUS       BBa_M45102              1298 bp    DNA     linear       25-Nov-2024
DEFINITION  Cobalt detection Biobrick. RFP made in presence of Cobalt. Note 
            the receptor also works in the prese
ACCESSION   BBa_M45102
VERSION     BBa_M45102.1  
FEATURES             Location/Qualifiers
     CDS             1..279
                     /label=RcnR
     promoter        1..426
                     /label=BBa_K540001
     RBS             435..449
                     /label=RBS-1\Strong
     RBS             442..446
                     /label=RBS
     misc_feature    435..449
                     /label=BBa_B0030
     RBS             435..449
                     /label=BBa_B0030
     CDS             456..1130
                     /label=mrfp1
     misc_feature    1137..1161
                     /label=Help:Barcodes
     CDS             456..1161
                     /label=BBa_E1010
     stem_loop       1181..1224
                     /label=stem_loop
     misc_feature    1170..1249
                     /label=BBa_B0010
     terminator      1170..1249
                     /label=BBa_B0010
     stem_loop       1265..1284
                     /label=T7 TE
     misc_feature    1291..1291
                     /label=stop
     polyA_site      1285..1298
                     /label=polya
     misc_feature    1258..1298
                     /label=BBa_B0012
     terminator      1258..1298
                     /label=BBa_B0012
     terminator      1170..1298
                     /label=BBa_B0015
ORIGIN
        1 ttattattat ttgatatatg aatccagcac cttcagaacg acatccagat cttcttcacg
       61 ttttagctca tccccctggt gaacgatgtg ttccgtcaga tgacctttaa tcacttcccg
      121 catcagaccg tttaccgcgc cacggatagc agcaatctgt tgtaaaactg ctgcgcattc
      181 gtgcggctcg tcgagcattt tcttgagcgc cacgacctgg ccctgaatct tactggcacg
      241 cgctttcagt ttctgtttat cacggattgt atgagacatg gcaacacctg gttaacaaga
      301 atatgaaaaa tcatagcact attaatctac tggggggtag tatcaggtac tgggggggag
      361 tagaatcaga ttgccgaatt aatactaaga attattatca tgaccgaatt tacaactctt
      421 cttcagtact agagattaaa gaggagaaat actagatggc ttcctccgaa gacgttatca
      481 aagagttcat gcgtttcaaa gttcgtatgg aaggttccgt taacggtcac gagttcgaaa
      541 tcgaaggtga aggtgaaggt cgtccgtacg aaggtaccca gaccgctaaa ctgaaagtta
      601 ccaaaggtgg tccgctgccg ttcgcttggg acatcctgtc cccgcagttc cagtacggtt
      661 ccaaagctta cgttaaacac ccggctgaca tcccggacta cctgaaactg tccttcccgg
      721 aaggtttcaa atgggaacgt gttatgaact tcgaagacgg tggtgttgtt accgttaccc
      781 aggactcctc cctgcaagac ggtgagttca tctacaaagt taaactgcgt ggtaccaact
      841 tcccgtccga cggtccggtt atgcagaaaa aaaccatggg ttgggaagct tccaccgaac
      901 gtatgtaccc ggaagacggt gctctgaaag gtgaaatcaa aatgcgtctg aaactgaaag
      961 acggtggtca ctacgacgct gaagttaaaa ccacctacat ggctaaaaaa ccggttcagc
     1021 tgccgggtgc ttacaaaacc gacatcaaac tggacatcac ctcccacaac gaagactaca
     1081 ccatcgttga acagtacgaa cgtgctgaag gtcgtcactc caccggtgct taataacgct
     1141 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc
     1201 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
     1261 cactggctca ccttcgggtg ggcctttctg cgtttata
//