BBa_M45123
type: ComponentDefinition
persistent identity: BBa M45123
display id: BBa_M45123
version: 1
was generated by: iGEM to SBOL conversion
description: Uranium Reducing and Detection Device for E. coli
created: 2014-04-16T11:00:00Z
modified: 2015-05-08T01:14:08Z
mutable provenance: asdfasdf
owned by: james
owned by: myers
mutable description: asdfasdf
discontinued: false
dominant: false
experience: experience/None
group u list: _1855_
m user id: 0
owner id: 20933
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: asdfasdf
star: false
creator: Sherissa Ward
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_B0010
component: BBa_M45116
component: BBa_B0012
component: BBa_B0034
component: BBa_M45113
component: BBa_M45133
component: BBa_K208005
sequence annotation: BBa_M45113
sequence annotation: BBa_B0034
sequence annotation: BBa_K208005
sequence annotation: BBa_M45133
sequence annotation: BBa_M45116
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_M45123_sequence/1

BBa_B0010
type: Component
persistent identity: component2372624
display id: component2372624
version: 1
top level: BBa_M45123
definition: BBa_B0010
access: public

BBa_M45116
type: Component
persistent identity: component2372623
display id: component2372623
version: 1
top level: BBa_M45123
definition: BBa_M45116
access: public

BBa_B0012
type: Component
persistent identity: component2372626
display id: component2372626
version: 1
top level: BBa_M45123
definition: BBa_B0012
access: public

BBa_B0034
type: Component
persistent identity: component2372612
display id: component2372612
version: 1
top level: BBa_M45123
definition: BBa_B0034
access: public

BBa_M45113
type: Component
persistent identity: component2372610
display id: component2372610
version: 1
top level: BBa_M45123
definition: BBa_M45113
access: public

BBa_M45133
type: Component
persistent identity: component2372619
display id: component2372619
version: 1
top level: BBa_M45123
definition: BBa_M45133
access: public

BBa_K208005
type: Component
persistent identity: component2372615
display id: component2372615
version: 1
top level: BBa_M45123
definition: BBa_K208005
access: public

BBa_M45113
type: SequenceAnnotation
persistent identity: annotation2372610
display id: annotation2372610
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372610/range2372610/1
component: BBa_M45113

https://synbiohub.org/public/igem/BBa_M45123/annotation2372610/range2372610/1
type: Range
persistent identity: range2372610
display id: range2372610
version: 1
top level: BBa_M45123
direction: direction/reverse
start: 1
end: 974
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2372612
display id: annotation2372612
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372612/range2372612/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M45123/annotation2372612/range2372612/1
type: Range
persistent identity: range2372612
display id: range2372612
version: 1
top level: BBa_M45123
direction: direction/reverse
start: 983
end: 994
orientation: inline

BBa_K208005
type: SequenceAnnotation
persistent identity: annotation2372615
display id: annotation2372615
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372615/range2372615/1
component: BBa_K208005

https://synbiohub.org/public/igem/BBa_M45123/annotation2372615/range2372615/1
type: Range
persistent identity: range2372615
display id: range2372615
version: 1
top level: BBa_M45123
direction: direction/reverse
start: 1001
end: 1126
orientation: inline

BBa_M45133
type: SequenceAnnotation
persistent identity: annotation2372619
display id: annotation2372619
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372619/range2372619/1
component: BBa_M45133

https://synbiohub.org/public/igem/BBa_M45123/annotation2372619/range2372619/1
type: Range
persistent identity: range2372619
display id: range2372619
version: 1
top level: BBa_M45123
direction: direction/reverse
start: 1133
end: 1699
orientation: inline

BBa_M45116
type: SequenceAnnotation
persistent identity: annotation2372623
display id: annotation2372623
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372623/range2372623/1
component: BBa_M45116

https://synbiohub.org/public/igem/BBa_M45123/annotation2372623/range2372623/1
type: Range
persistent identity: range2372623
display id: range2372623
version: 1
top level: BBa_M45123
direction: direction/reverse
start: 1706
end: 2422
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation2372624
display id: annotation2372624
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372624/range2372624/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_M45123/annotation2372624/range2372624/1
type: Range
persistent identity: range2372624
display id: range2372624
version: 1
top level: BBa_M45123
direction: direction/forward
start: 2431
end: 2510
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation2372626
display id: annotation2372626
version: 1
top level: BBa_M45123
location: https://synbiohub.org/public/igem/BBa_M45123/annotation2372626/range2372626/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_M45123/annotation2372626/range2372626/1
type: Range
persistent identity: range2372626
display id: range2372626
version: 1
top level: BBa_M45123
direction: direction/forward
start: 2519
end: 2559
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_K208005
type: ComponentDefinition
persistent identity: BBa K208005
display id: BBa_K208005
version: 1
was generated by: iGEM to SBOL conversion
description: TorA Signal Peptide - Silver Fusion Compatible
created: 2009-10-11T11:00:00Z
modified: 2015-05-08T01:11:24Z
mutable provenance: synthetically produced
owned by: james
owned by: myers
mutable description: torA
discontinued: false
dominant: false
experience: experience/Works
group u list: _310_
m user id: 0
owner id: 3473
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: silver fusion
star: false
creator: USU iGEM 2009
type: DnaRegion
role: partType/Tag
role: SO:0000324
sequence annotation: Start
sequence annotation: TorA
sequence: https://synbiohub.org/public/igem/BBa_K208005_sequence/1

Start
type: SequenceAnnotation
persistent identity: annotation2062222
display id: annotation2062222
version: 1
top level: BBa_K208005
location: https://synbiohub.org/public/igem/BBa_K208005/annotation2062222/range2062222/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_K208005/annotation2062222/range2062222/1
type: Range
persistent identity: range2062222
display id: range2062222
version: 1
top level: BBa_K208005
direction: direction/forward
start: 1
end: 3
orientation: inline

TorA
type: SequenceAnnotation
persistent identity: annotation2034623
display id: annotation2034623
version: 1
top level: BBa_K208005
location: https://synbiohub.org/public/igem/BBa_K208005/annotation2034623/range2034623/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_K208005/annotation2034623/range2034623/1
type: Range
persistent identity: range2034623
display id: range2034623
version: 1
top level: BBa_K208005
direction: direction/forward
start: 1
end: 126
orientation: inline

BBa_M45133
type: ComponentDefinition
persistent identity: BBa M45133
display id: BBa_M45133
version: 1
was generated by: iGEM to SBOL conversion
description: Chromate and Uranium Reductase, ChrR30 Silver-fusion compatible
created: 2014-04-06T11:00:00Z
modified: 2015-05-08T01:14:09Z
mutable provenance: asda
owned by: james
owned by: myers
mutable description: adas
discontinued: false
dominant: false
experience: experience/None
group u list: _1855_
m user id: 0
owner id: 20931
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: ada
star: false
creator: Ozkan Fidan
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Coding Sequence
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M45133_sequence/1

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2372201
display id: annotation2372201
version: 1
top level: BBa_M45133
location: https://synbiohub.org/public/igem/BBa_M45133/annotation2372201/range2372201/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_M45133/annotation2372201/range2372201/1
type: Range
persistent identity: range2372201
display id: range2372201
version: 1
top level: BBa_M45133
direction: direction/forward
start: 1
end: 567
orientation: inline

Start Codon
type: SequenceAnnotation
persistent identity: annotation2372200
display id: annotation2372200
version: 1
top level: BBa_M45133
location: https://synbiohub.org/public/igem/BBa_M45133/annotation2372200/range2372200/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_M45133/annotation2372200/range2372200/1
type: Range
persistent identity: range2372200
display id: range2372200
version: 1
top level: BBa_M45133
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2372202
display id: annotation2372202
version: 1
top level: BBa_M45133
location: https://synbiohub.org/public/igem/BBa_M45133/annotation2372202/range2372202/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M45133/annotation2372202/range2372202/1
type: Range
persistent identity: range2372202
display id: range2372202
version: 1
top level: BBa_M45133
direction: direction/forward
start: 565
end: 567
orientation: inline

BBa_M45113
type: ComponentDefinition
persistent identity: BBa M45113
display id: BBa_M45113
version: 1
was generated by: iGEM to SBOL conversion
description: Uranium Inducible (urcA) Promoter
created: 2014-04-16T11:00:00Z
modified: 2015-05-08T01:14:08Z
mutable provenance: This part comes from Caulobacter crescentus CB15 section 318 of 359 of the complete genome, subsection 7998-8971, which may be found in Genbank at accession number AE005992.1 (Hillson et al., US Patent, Feb. 6 2008). Reference: Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Heavy Metal Biosensor. US Patent Application Publication. US20110117590. Feb. 6 2008. This reference was retrieved from: file:///C:/Users/Owner/Desktop/US20110117590.pdf
owned by: james
owned by: myers
mutable description: The promoter was designated urcA for uranium response in the bacterium Caulobacter. It is activated in the presence of the uranyl cation, a soluble form of uranium (Hillson et al., 2007). The uranyl ion (UO2,2+ ) is the most water-soluble and bioavailable form of uranium and poses the greatest threat to human health. Due to the ease of uranyl ion spread through groundwater systems, most bioremediation strategies attempt to prevent contaminating uranium spread by utilizing microorganisms to reduce the oxidation state of uranium from U(VI), found in uranyl, to less soluble forms of uranium, including U(IV)(Hillson et al., 2007). The urcA promoter is speciﬁc for uranium and has little cross speciﬁcity for nitrate (<400 M), lead (<150 M), cadmium (<48 M), or chromium (<41.6 M). It was induced 27.5-fold under uranium stress, but was not upregulated in response to other heavy metals in the test screen. For this reason, the urcA promoter was selected as a candidate to drive uranium reporter constructs(Hillson et al., 2007). Usually promoters from Caulobacter species will include at least one uranium-specific m_5 motif sequence. The urcA promoter contains two matches to this motif, located 107 and 55 bp upstream of the putative +1 site (Hillson et al., 2007). Reference: Nathan J. Hillson, Ping Hu, Gary L. Andersen and Lucy Shapiro. Caulobacter crescentus as a Whole-Cell Uranium Biosensor. Appl. Environ. Microbiol. 2007, 73(23):7615. DOI: 10.1128/AEM.01566-07. This reference was retrieved from: file:///C:/Users/Owner/Desktop/Appl.%20Environ.%20Microbiol.-2007-Hillson-7615-21.pdf
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 20936
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence complied with RFC-10 standard assembly, so no design considerations had to be considered during the creation of the sequence as a biobrick.
star: false
creator: Federico C. Rodriguez
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: Promoter
sequence annotation: Uranium-inducible m_5 motif 1
sequence annotation: Uranium-inducible m_5 motif 2
sequence: https://synbiohub.org/public/igem/BBa_M45113_sequence/1

Promoter
type: SequenceAnnotation
persistent identity: annotation2372450
display id: annotation2372450
version: 1
top level: BBa_M45113
location: https://synbiohub.org/public/igem/BBa_M45113/annotation2372450/range2372450/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_M45113/annotation2372450/range2372450/1
type: Range
persistent identity: range2372450
display id: range2372450
version: 1
top level: BBa_M45113
direction: direction/forward
start: 1
end: 974
orientation: inline

Uranium-inducible m_5 motif 1
type: SequenceAnnotation
persistent identity: annotation2372452
display id: annotation2372452
version: 1
top level: BBa_M45113
location: https://synbiohub.org/public/igem/BBa_M45113/annotation2372452/range2372452/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M45113/annotation2372452/range2372452/1
type: Range
persistent identity: range2372452
display id: range2372452
version: 1
top level: BBa_M45113
direction: direction/forward
start: 825
end: 851
orientation: inline

Uranium-inducible m_5 motif 2
type: SequenceAnnotation
persistent identity: annotation2372451
display id: annotation2372451
version: 1
top level: BBa_M45113
location: https://synbiohub.org/public/igem/BBa_M45113/annotation2372451/range2372451/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_M45113/annotation2372451/range2372451/1
type: Range
persistent identity: range2372451
display id: range2372451
version: 1
top level: BBa_M45113
direction: direction/forward
start: 877
end: 903
orientation: inline

BBa_M45116
type: ComponentDefinition
persistent identity: BBa M45116
display id: BBa_M45116
version: 1
was generated by: iGEM to SBOL conversion
description: GFPuv
created: 2014-04-18T11:00:00Z
modified: 2015-05-08T01:14:08Z
mutable provenance: GFPuv comes was originally isolated from the bioluminescent jellyfish Aequorea Victoria (Chin et al.).
owned by: james
owned by: myers
mutable description: Green fluorescent protein (GFP) is a 27 kDa protein of 238 amino acids in length. GFP is intrinsically fluorescent and hence is ideal as a non-invasive marker in living cells. It is used as a cell lineage tracer, reporter of gene expression, a measure of protein-protein interactions, and as an intracellular pH indicator. GFP can function as a protein tag as it tolerates N- and C-terminal fusion to a broad variety of proteins (Chin et al.). Wild-type GFP fluoresces maximally when excited at 395 nm, with a minor peak at 475 nm. Fluorescence emission peaks at 509 nm. It is stable within a range of pH 5.5 to 12. Denaturation occurs at temperatures above 70??C (Chin et al.). Several variants with improved absorption or emission spectra, or increased the fluorescence intensity, have since been developed. Variants also exhibit different thermosensitivities (Chin et al.). As stated before, the maximum excitation spectra of GFPuv is 395nm while its maximum emission spectra is 509nm, which is similar to that of wild-type GFP. Compared to wild-type GFP, GFPuv contains three amino acid substitutions (Phe-99 to Ser, Met-153 to Thr, and Val-163 to Ala [based on the amino acid numbering of wild-type GFP]), none of which alter the chromophore sequence. The main advantage of this variant over wild-type GFP is its large amount of fluorescence when excited by ultra-violet (UV) light at 395nm. GFPuv is 18 times brighter than wild-type GFP under ultra-violet excitation and can easily be detected by the eye without the need for specialized equipment. Furthermore, GFPuv expressed in E. coli is a soluble, fluorescent protein in contrast to wild-type GFP, much of which may be expressed as a nonfluorescent protein in inclusion bodies. Consequently, the GFPuv gene is expressed very efficiently in E. coli (Chin et al.). Reference: Chin et al. Characterization of Green Fluorescent Protein. Retrieved from: http://staff.science.nus.edu.sg/~scilooe/srp_2003/sci_paper/dbs/research%20_paper/ngeow_ka…
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 20936
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The sequence complied with RFC-10 assembly compatibility as a requirement for Utah State University Synthetic Biology class, so no design considerations had to be taken into account during the creation of the sequence as a biobrick.
star: false
creator: Federico C. Rodriguez
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start Codon
sequence annotation: Coding Sequence
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M45116_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2372548
display id: annotation2372548
version: 1
top level: BBa_M45116
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372548/range2372548/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M45116/annotation2372548/range2372548/1
type: Range
persistent identity: range2372548
display id: range2372548
version: 1
top level: BBa_M45116
direction: direction/forward
start: 1
end: 3
orientation: inline

Coding Sequence
type: SequenceAnnotation
persistent identity: annotation2372547
display id: annotation2372547
version: 1
top level: BBa_M45116
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372547/range2372547/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_M45116/annotation2372547/range2372547/1
type: Range
persistent identity: range2372547
display id: range2372547
version: 1
top level: BBa_M45116
direction: direction/forward
start: 1
end: 717
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2372549
display id: annotation2372549
version: 1
top level: BBa_M45116
location: https://synbiohub.org/public/igem/BBa_M45116/annotation2372549/range2372549/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M45116/annotation2372549/range2372549/1
type: Range
persistent identity: range2372549
display id: range2372549
version: 1
top level: BBa_M45116
direction: direction/forward
start: 715
end: 717
orientation: inline

https://synbiohub.org/public/igem/BBa_M45123_sequence/1
type: Sequence
persistent identity: BBa M45123 sequence
display id: BBa_M45123_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccggccgcacgcaagggcagatcatcggcctcggcgaaggttcgccccaggaagaagctgggattgagcggcttgtcacccttccggatctcgaaatggagatgcgagcccgaggaccgtccggaattgccgacgaaggcgacaatgtcgcctcgccgcaaataggcgccgcgcttcacgctacgggcgggacgggccagatgagcgtacagggtcgacagaccgcccttgtgcaccacaaggacatagcggccataggtggcgctgaccccggtggcctttacgacgccaggggccgcgaccttaacagctgcgccggcgggcgctgcgatatcaacgccctggtggagccgaccgctttcctcccacggcatctgtctcaagccgaagggcgaattgatgacccgccccggcaagggtgcgtcaaagacgaaggccgggggcggcgcctggacctcgggctccggtgcgggttgcgccaccgcagggatcgccgagctggtcggcgcgcgcgcaatccactcgctcatcgcgaccgcgccgttcaacgcgatcaccatcgcagcgatacccagcatcgagaagagcgcgacgcgcaagtgctgcggcgatagcgccaagctcatagagaccaaaaccacttcctcttcgacgcccggtcagtcgccaggaccgcgtctggacggttttgctctcatacttgacctttcgggatggtgaatcgacggcgtgcatgaatgtcgcatcgggcggaacggggcgtcgattaaccctttgcaaaccatatactcaaacgacccaagcaatatggtcacaaaaacttcaaacattacagactgtttagaatattaaagccccgtaattctcttaattacgcgtcatgactgaggtgtaacgagacttcgcgagaacccgaatgtatccaatattcatcggcgcagcgaacagcgcccagccagagggatacttcaatactagagaaagaggagaaatactagatgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtcgccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcgtactagatgtctgaaaaattgcaggtggttacgttactggggagcctgcgcaaaggctcatttaatggcatggttgcacgtaccctgccgaaaattgctccggcgagcatggaagtcaatgcgttaccatccattgccgacattcccttgtatgacgctgacgtacagcaggaagaaggttttccagcaacggttgaagctctggcggaacagatccgtcaggctgacggtgtggtgatcgtcacgccggaatataactactcggtaccgggtgggctgaaaaatgccatcgactggctttcccgcctgccggatcaaccgctggccggtaaaccggtattgattcagaccagctcaatgggcgtgattggcggcgcgcgctgtcaggatcacctgcgccagattctggttttcctcgatgcaatggtgatgaacaagccggaatttatgggcagcgtgattcagaccaaagttgatccgcaaaccggagaagtgattgatcagggtacgctggaccacctgaccgggcaattgaccgcatttggtgagtttattcagcgagttaagatctaatactagatgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatat…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K208005_sequence/1
type: Sequence
persistent identity: BBa K208005 sequence
display id: BBa_K208005_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaacaataacgatctctttcaggcatcacgtcggcgttttctggcacaactcggcggcttaaccgtcgccgggatgctggggccgtcattgttaacgccgcgacgtgcgactgcggcgcaagcg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45133_sequence/1
type: Sequence
persistent identity: BBa M45133 sequence
display id: BBa_M45133_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgtctgaaaaattgcaggtggttacgttactggggagcctgcgcaaaggctcatttaatggcatggttgcacgtaccctgccgaaaattgctccggcgagcatggaagtcaatgcgttaccatccattgccgacattcccttgtatgacgctgacgtacagcaggaagaaggttttccagcaacggttgaagctctggcggaacagatccgtcaggctgacggtgtggtgatcgtcacgccggaatataactactcggtaccgggtgggctgaaaaatgccatcgactggctttcccgcctgccggatcaaccgctggccggtaaaccggtattgattcagaccagctcaatgggcgtgattggcggcgcgcgctgtcaggatcacctgcgccagattctggttttcctcgatgcaatggtgatgaacaagccggaatttatgggcagcgtgattcagaccaaagttgatccgcaaaccggagaagtgattgatcagggtacgctggaccacctgaccgggcaattgaccgcatttggtgagtttattcagcgagttaagatctaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45113_sequence/1
type: Sequence
persistent identity: BBa M45113 sequence
display id: BBa_M45113_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggccggccgcacgcaagggcagatcatcggcctcggcgaaggttcgccccaggaagaagctgggattgagcggcttgtcacccttccggatctcgaaatggagatgcgagcccgaggaccgtccggaattgccgacgaaggcgacaatgtcgcctcgccgcaaataggcgccgcgcttcacgctacgggcgggacgggccagatgagcgtacagggtcgacagaccgcccttgtgcaccacaaggacatagcggccataggtggcgctgaccccggtggcctttacgacgccaggggccgcgaccttaacagctgcgccggcgggcgctgcgatatcaacgccctggtggagccgaccgctttcctcccacggcatctgtctcaagccgaagggcgaattgatgacccgccccggcaagggtgcgtcaaagacgaaggccgggggcggcgcctggacctcgggctccggtgcgggttgcgccaccgcagggatcgccgagctggtcggcgcgcgcgcaatccactcgctcatcgcgaccgcgccgttcaacgcgatcaccatcgcagcgatacccagcatcgagaagagcgcgacgcgcaagtgctgcggcgatagcgccaagctcatagagaccaaaaccacttcctcttcgacgcccggtcagtcgccaggaccgcgtctggacggttttgctctcatacttgacctttcgggatggtgaatcgacggcgtgcatgaatgtcgcatcgggcggaacggggcgtcgattaaccctttgcaaaccatatactcaaacgacccaagcaatatggtcacaaaaacttcaaacattacagactgtttagaatattaaagccccgtaattctcttaattacgcgtcatgactgaggtgtaacgagacttcgcgagaacccgaatgtatccaatattcatcggcgcagcgaacagcgcccagccagagggatacttcaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45116_sequence/1
type: Sequence
persistent identity: BBa M45116 sequence
display id: BBa_M45116_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgagtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttctcttatggtgttcaatgcttttcccgttatccggatcatatgaaacggcatgactttttcaagagtgccatgcccgaaggttatgtacaggaacgcactatatctttcaaagatgacgggaactacaagacgcgtgctgaagtcaagtttgaaggtgatacccttgttaatcgtatcgagttaaaaggtattgattttaaagaagatggaaacattctcggacacaaactcgagtacaactataactcacacaatgtatacatcacggcagacaaacaaaagaatggaatcaaagctaacttcaaaattcgccacaacattgaagatggatccgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtcgacacaatctgccctttcgaaagatcccaacgaaaagcgtgaccacatggtccttcttgagtttgtaactgctgctgggattacacatggcatggatgagctctacaaataa
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z