LOCUS BBa_M45178 5927 bp DNA linear 22-Dec-2024 DEFINITION Composite radiation biosensor ACCESSION BBa_M45178 VERSION BBa_M45178.1 FEATURES Location/Qualifiers promoter 1..244 /label=BBa_I766555 RBS 253..270 /label=BBa_K165002 misc_feature 277..279 /label=Start codon misc_feature 880..882 /label=Stop codon CDS 277..882 /label=BBa_M45189 RBS 891..908 /label=BBa_K165002 misc_feature 915..917 /label=Start codon misc_feature 1503..1505 /label=Stop codon CDS 915..1505 /label=Coding sequence CDS 915..1505 /label=BBa_M45176 misc_feature 1514..1521 /label=Linker 4 polyA_site 1522..1746 /label=BGH terminator 1514..1746 /label=BBa_K678019 promoter 1753..2811 /label=BBa_M45188 RBS 2820..2837 /label=BBa_K165002 misc_feature 2844..2846 /label=Start codon misc_feature 3501..3503 /label=Stop codon (added) CDS 2844..3503 /label=Coding sequence CDS 2844..3503 /label=BBa_M45175 RBS 3512..3529 /label=BBa_K165002 misc_feature 3536..3538 /label=Start codon misc_feature 5682..5686 /label=Stop codon CDS 3536..5686 /label=Coding sequence CDS 3536..5686 /label=BBa_M45172 misc_feature 5695..5702 /label=Linker 4 polyA_site 5703..5927 /label=BGH terminator 5695..5927 /label=BBa_K678019 ORIGIN 1 cagatccgcc aggcgtgtat atatagcgtg gatggccagg caactttagt gctgacacat 61 acaggcatat atatatgtgt gcgacgacac atgatcatat ggcatgcatg tgctctgtat 121 gtatataaaa ctcttgtttt cttcttttct ctaaatattc tttccttata cattaggacc 181 tttgcagcat aaattactat acttctatag acacacaaac acaaatacac acactaaatt 241 aatatactag agcccgccgc caccatggag tactagatga aagcactgac cgcacgtcag 301 caggaagttt ttgatctgat tcgtgatcat attagccaga ccggtatgcc gccgacccgt 361 gcagaaattg cacagcgtct gggttttcgt agcccgaatg cagcagaaga acatctgaaa 421 gcactggcac gtaaaggtgt tattgaaatt gttagcggtg caagccgtgg tattcgtctg 481 ttacaggaag aagaagaagg tctgccgctg gttggtcgtg ttgcagcagg tgaaccgctg 541 ctggcacagc agcatattga aggtcattat caggttgatc cgagcctgtt taaaccgaat 601 gcagattttc tgctgcgtgt tagcggtatg agcatgaaag atattggtat tatggatggt 661 gatctgctgg cagttcataa aacccaggat gttcgtaatg gtcaggttgt tgttgcacgt 721 attgatgatg aagttaccgt taaacgtctg aaaaaacagg gtaataaagt tgaactgctg 781 ccggaaaata gcgaatttaa accgattgtt gttgatctgc gtcagcagag ctttaccatt 841 gaaggtctgg cagttggtgt tattcgtaat ggtgattggc tgtactagag cccgccgcca 901 ccatggagta ctagatggcg tactccactc tgttcataat tgcattgacc gccgttgtca 961 ctcaagcttc ctcaactcaa aaatctaacc taacttttac gttggcagat ttcgttggag 1021 actggcaaca gacagctgga tacaaccaag atcaagtgtt agaacaagga ggattgtcta 1081 gtctgttcca agccctggga gtgtcagtca cgcccataca gaaagttgta ctgtctgggg 1141 agaatgggtt aaaagctgat attcatgtca taatacctta cgagggactc agtggttttc 1201 aaatgggtct aattgaaatg atcttcaaag ttgtttaccc cgtggatgat catcatttca 1261 agattattct ccattatggt acactcgtta ttgacggtgt aacacccaac atgattgact 1321 actttggaag accttaccct ggaattgctg tatttgacgg caagcagatc acagttactg 1381 gaactctgtg gaacggcaac aagatctatg atgagaggct aatcaaccct gatggttcac 1441 tcctcttcag agttactatc aatggagtca cgggatggag gctttgcgag aacattcttg 1501 cctaatacta gagattccga tctgtgcctt ctagttgcca gccatctgtt gtttgcccct 1561 cccccgtgcc ttccttgacc ctggaaggtg ccactcccac tgtcctttcc taataaaatg 1621 aggaaattgc atcgcattgt ctgagtaggt gtcattctat tctggggggt ggggtggggc 1681 aggacagcaa gggggaggat tgggaagaca atagcaggca tgctggggat gcggtgggct 1741 ctatggtact agatggcaat tgatgaaaat aaacagaaag cactggcagc agcactgggt 1801 cagattgaaa aacagtttgg taaaggtagc attatgcgtc tgggtgaaga tcgtagcatg 1861 gatgttgaaa ccattagcac cggtagcctg agcctggata ttgcactggg tgcaggtggt 1921 ctgccgatgg gtcgtattgt tgaaatttat ggtccggaaa gcagcggtaa aaccaccctg 1981 accttacagg ttattgcagc agcacagcgt gaaggtaaaa cctgtgcatt tattgatgca 2041 gaacatgcac tggatccgat ttatgcacgt aaactgggtg ttgatattga taatctgctg 2101 tgtagccagc cggataccgg tgaacaggca ctggaaattt gtgatgcact ggcacgtagc 2161 ggtgcagttg atgttattgt tgttgatagc gttgcagcac tgaccccgaa agcagaaatt 2221 gaaggtgaaa ttggtgatag ccatatgggt ctggcagcac gtatgatgag ccaggcaatg 2281 cgtaaactgg caggtaatct gaaacagagc aataccctgc tgatttttat taatcagatt 2341 cgtatgaaaa ttggtgttat gtttggtaat ccggaaacca ccaccggtgg taatgcactg 2401 aaattttatg caagcgttcg tctggatatt cgtcgtattg gtgcagttaa agaaggtgaa 2461 aatgttgttg gtagcgaaac ccgtgttaaa gttgttaaaa ataaaattgc agcaccgttt 2521 aaacaggcag aatttcagat tctgtatggt gaaggtatta atttttatgg tgaactggtt 2581 gatctgggtg ttaaagaaaa actgattgaa aaagcaggtg catggtatag ctataaaggt 2641 gaaaaaattg gtcagggtaa agcaaatgca accgcatggc tgaaagataa tccggaaacc 2701 gcaaaagaaa ttgaaaaaaa agttcgtgaa ctgctgctga gcaatccgaa tagcaccccg 2761 gattttagcg ttgatgatag cgaaggtgtt gcagaaacca atgaagattt ttactagagc 2821 ccgccgccac catggagtac tagatggata ttaaggtggt cttcacactg gtgttctccg 2881 ccctggtgca ggctaagagc acggagttcg acccgaatat cgatatcgtg gggttagaag 2941 gcaagttcgg gatcacgaat ctggagacag atctgttcac gatctgggag acaatggaag 3001 tcatgatcaa ggccgacatc gccgacaccg accgagcctc caattttgtg gccacagaga 3061 ccgatgctaa caggggcaaa atgccaggaa agaaactccc tctcgccgtg ataatggaaa 3121 tggaagccaa cgcattcaaa gccggctgca ctaggggctg tttaatatgc ctgagtaaga 3181 taaaatgtac cgccaagatg aaggtgtaca ttcccggaag atgtcacgat tacgggggtg 3241 acaaaaaaac cgggcaagcc gggatcgtgg gcgccatcgt ggatattccc gaaattagcg 3301 ggttcaagga gatggcgccc atggaacagt ttatcgcgca ggttgaccgt tgtgcctcat 3361 gtaccacagg ctgcctgaaa ggcttagcca acgtgaagtg ctccgagctg ttgaaaaagt 3421 ggctgcctga ccgatgcgcc tctttcgctg ataaaatcca gaaagaggtg cataacatta 3481 aaggaatggc gggagaccga tagtactaga gcccgccgcc accatggagt actagatgaa 3541 cgtgatcgct attctgaacc atatgggtgt atactttaag gaagagccta tcagggaact 3601 ccatcgcgca cttgagcggt taaacttcca gatagtttac cccaacgatc gagatgatct 3661 gctgaagtta atcgagaata acgctcggct gtgtggagtg atcttcgact gggataagta 3721 caatctggaa ctgtgtgaag agatttctaa aatgaacgag aatctaccgc tctacgcctt 3781 cgccaacacc tatagcacct tagatgtcag tctgaatgat ctgaggctcc agatcagctt 3841 tttcgagtac gccctgggcg ctgctgaaga catagcaaac aagatcaaac agaccacgga 3901 cgaatacatc aatacaattc tccctcccct gaccaaagct ctcttcaagt atgtaagaga 3961 gggcaagtac accttttgca cgccaggaca catgggggga accgctttcc agaaatcacc 4021 cgtaggatcc ctgttctacg atttttttgg gcctaatacc atgaagagtg acatcagcat 4081 atccgtttca gaactaggga gtcttctgga ccacagtggc cctcacaaag aggccgagca 4141 gtacattgcc agggtgttta acgccgatag atcctatatg gtcactaatg ggacttcgac 4201 cgctaataag atcgtcggca tgtattcagc tcccgccggg tccactatct taatcgaccg 4261 caattgccac aagtctttga cccacctaat gatgatgtcc gacgttacac ctatctattt 4321 ccgaccgact aggaacgcct acgggatcct tggtggcatt cctcagtcgg agtttcagca 4381 tgctaccatc gccaaaaggg tgaaggaaac tcccaatgcg acatggccgg tgcatgccgt 4441 gatcactaac tccacatatg atggactcct ttataatacg gactttatca agaagacgct 4501 cgacgtaaaa tcgattcatt ttgatagcgc ctgggtgcct tacaccaatt tcagccccat 4561 ctatgagggg aagtgcggca tgtcgggggg ccgcgtggag ggaaaggtta tctacgagac 4621 ccagtccacc cacaaactgc tagcagcctt cagccaggcc tctatgattc atgtgaaagg 4681 cgacgttaat gaggagacct ttaacgaggc atacatgatg catactacca cctctccaca 4741 ttatggtatc gtcgcctcca ccgaaacagc ggcagccatg atgaagggaa atgccgggaa 4801 acggcttatc aacggttcca tcgagcgcgc catcaaattc aggaaggaaa tcaagagatt 4861 gcgcaccgaa tcagacggat ggttcttcga cgtgtggcag cccgatcaca tcgatactac 4921 tgaatgctgg ccgctgagaa gcgactctac ttggcacgga ttcaagaaca tcgataatga 4981 acacatgtac ctcgatccca tcaaagtgac cctcttaacc cccggaatgg aaaaagacgg 5041 aacaatgtcc gactttggta tcccggcgtc tatcgtggct aaatatctcg acgagcacgg 5101 aatcgtggtg gagaagactg gaccttataa tctcctgttc ctgttttcta taggcatcga 5161 caagacaaag gccctatctc tgctgagggc cttaaccgac ttcaagcggg cttttgatct 5221 gaacctgcgg gtgaagaaca tgctgccttc cctttatagg gaggatccag agttctacga 5281 aaatatgcgc atccaggagc tcgcgcagaa catccataaa cttatagtcc atcataacct 5341 cccagacctg atgtataggg ctttcgaggt tttacctacc atggtgatga ccccctacgc 5401 tgcttttcaa aaggagctcc acggtatgac tgaggaagtg tacttggatg agatggtagg 5461 ccgcattaat gctaacatga tacttccata cccccctggc gtgcccttgg tgatgcccgg 5521 cgaaatgata accgaagagt cccggcctgt gctggaattt cttcaaatgc tctgcgagat 5581 tggcgctcac taccccggct tcgagaccga tattcacggc gcctacagac aggctgacgg 5641 ccggtatacc gtcaaagtgc taaaagaaga atctaagaag taataatact agagattccg 5701 atctgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac 5761 cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg catcgcattg 5821 tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca agggggagga 5881 ttgggaagac aatagcaggc atgctgggga tgcggtgggc tctatgg //