Composite of synthetic analogs, eukaryotic and prokaryotic promoters, and eukaryotic coding sequences.
(For class project) A composite device that employs the LexA/RecA system as an ionizing radiation biosensor, triggering luminescence response from Metridia longa luciferase and odor response from lysine decarboxylase.
it is a promoter for the detection of DNA disruption
Coding region, Metridia longa secreted luciferase protein
Coding region of Metridia longa secreted luciferase protein. (For class project use.)
Coelenterazine analog for luciferase substrate
Mid-expression level constitutive promoter in yeast
The kozak sequence acts as a eukaryotic RBS. It is cloned directly between a promoter and coding region.
BGH poly A, terminator for use in mammalian cells
Compatible with the Plug'n'Play assembly standard
From E.coli (K12)cds. Sequence originally published by Meng,S.Y. and Bennett,G.N. in "Nucleotide sequence of the Escherichia coli cad operon: a system for neutralization of low extracellular pH" (1992)
Lysine decarboxylase consumes protons to metabolize L-lysine to cadaverine and CO2. Expression of the cadA gene is normally controlled by cadC. Lysine decarboxylase uses pyridoxal 5'-phosphate as a cofactor. This is a HYPOTHETICAL part that has not gone under any testing but has been changed to biobrick format.
Sequence has strangely enough been optimized for expression within the Felix cattus genome. It is suggested that it be optimized again for the desired clone species. Prefix and suffix RE sites have been removed from the sequence. Again this part has not been tested and is HYPOTHETICAL only.
Conversion of the iGEM parts registry to SBOL2.1