LOCUS       BBa_M45203              3461 bp    DNA     linear       19-Apr-2025
DEFINITION  Biosensor with Legionella Promoter (Device 2)
ACCESSION   BBa_M45203
VERSION     BBa_M45203.1  
FEATURES             Location/Qualifiers
     RBS             29..42
                     /label=Ribosome Binding Site
     RBS             29..42
                     /label=BBa_M45193
     misc_feature    49..51
                     /label=Start Codon
     misc_feature    1168..1170
                     /label=Stop Codon
     misc_feature    357..357
                     /label=Changed Base to C 
     misc_feature    501..501
                     /label=Changed Base to C
     misc_feature    936..936
                     /label=Changed Base to C
     misc_feature    1026..1026
                     /label=Changed Base to C
     CDS             49..1170
                     /label=Coding Region
     CDS             49..1170
                     /label=BBa_M45198
     misc_feature    1177..1179
                     /label=Start Codon 
     misc_feature    1275..1277
                     /label=Stop Codon 
     CDS             1177..1277
                     /label=Coding Region
     CDS             1177..1277
                     /label=BBa_M45196
     misc_feature    1284..1286
                     /label=start
     misc_feature    2250..2252
                     /label=stop
     CDS             1284..2252
                     /label=Coding Region
     CDS             1284..2252
                     /label=BBa_M45192
     misc_feature    2259..2261
                     /label=start
     misc_feature    2634..2636
                     /label=stop
     CDS             2259..2636
                     /label=Coding Region
     CDS             2259..2636
                     /label=BBa_M45195
     misc_feature    2643..2645
                     /label=Start Codon
     misc_feature    3360..3362
                     /label=Stop Codon
     CDS             2643..3362
                     /label=Coding Region
     misc_feature    2643..3362
                     /label=BBa_M45190
     terminator      3371..3461
                     /label=BBa_M45194
ORIGIN
        1 agcactactc ataaacatgt tactagagat taaagaggag aatactagat ggccgtcgac
       61 ttcaccccgc gcaccgtctt cagcatcggc ctgcgttcgc ccaagcagat ggccagggcc
      121 gaggcgcaga aggatgcgtt gaagcagagc gaggccagac aggccgaggc gcagcaggtg
      181 gaggacggca gcccggcggc cgaagtgcag cgtttcgtcc agtcgaccga cgaaatgtcg
      241 gcggtgatgg cccagttccg caaccggcgc gatttcgaca aaaaggtggg cacgctggcc
      301 gacagcttcg agcgggtgct ggacgaagac gcgctgccca aggcccagcg catcctccag
      361 gtggccaaga cccacggcgt gtcggccgag gagctgctgc gccaggcgcg cgcgctgttt
      421 cccgacgcca gcgacctggt gctggtgctg cgggagctgc tgcggcgcag gcagctggac
      481 gaaatcgtgc gcaagcggct ccaggcgctg ctggcgcagg tggaggcgga agcggagccc
      541 aagccgctga aggccggcat caactgcgcg ctgaaggcgc gcctgttcgg caaggcgctg
      601 aatctcagcc ccgggctgct gcgggccagt taccgccagt tcctggagag cgacgcgtcc
      661 gaggccgaaa tctacgccga ctgggtggcc agctacggct gccagcggcg ggcggcggtg
      721 ctggatttca tggaaggcgc gctgctggcc gacatcgatt cccaggacgc cagctgctcg
      781 cggctggagt tcggccacct gctggggcgg ctgacccagc tcaagctgct gcgctcggcc
      841 gacgtccagt tcgtcggccg catgctggcc gatccgctgg cgcgccgctt caacccggaa
      901 gaggccgact ggctggtgct gctgctcagc ctgctccagc aagccgacgg cgtcgacgcg
      961 ctgctggcgg acaccgtcgg ccgcgaggcc ttgctgagcc ggcacgccga ccgctccgcc
     1021 ttgctccagg cgctgttccg ggcctgcaag acgctgccgc ccaggctgtt tctggacgag
     1081 tcgtggcagc cggcgctgct ggaggcgctg cgcgagatgg ccggcatcgc ctaccggcat
     1141 gaacagatag agcggcggcg cgaaaactga tactagatgg tcacttttac ggttggccat
     1201 ttgtctctta cgttgcattt atcaatctgc tttttgatac agcagcacct cgctgctgct
     1261 ttttttattt gtgataatac tagatgttta tgagtagtcc acttccaaaa tccattgcca
     1321 gttcaaaaat gcaaggaagc gccctcttaa ctgctctatt tattatgaca ttggttgcta
     1381 taatcgccac tgccatgagt acacgagtgc aacaggacat ttacagaaca aggctagtga
     1441 ttgctcagga taaactatat cttgcctctc aggcagtcac tttctgggct ctcaatgaat
     1501 tacttgataa aaacaaccgc tttacaaaaa ccaaccagtt agggatggta gcccaatacc
     1561 ctaaaaatat ggaatccata tacaatcaag tacaattaag tggtggaatt tttgatttac
     1621 aagcgcgctt caacctcaat aacctgattg agaaaaaatc cattcccact ttaatgcacc
     1681 tgatcagtca cgcctactcg aaggcagccg gccaagaacg tgctaatata gccttgggag
     1741 taaagcattg gttgcttgct tatgatcttg gccgaggaga agatatttat acctcttatt
     1801 atctctctca gaaaccacct tactatccca gccatcaact tataaaaagt aaatctgagt
     1861 ttcgattgat aaaagatgta agtgcacccg cttatattgc ccttgaacct tttatcacag
     1921 ccttgcctga atcaacgagc attaacatta atactgctcc aaagcaaata ttgatgtcat
     1981 taggagatgg attaagtgac gcccaggcaa atgaattaat aactgctcgg ggggaaaatg
     2041 gtatcactga tctgaaagag atcaacgagt tattaaaaaa attcaatatt cccagtgacc
     2101 aaataactat tgaaagtcaa tattatctca gcgttgccta tgcaaagaat gatgaattta
     2161 gtttggttgt gtattctttg ttaaaacgca gtcgtgacag gaggggtaag ttattaacaa
     2221 gcgtcattag agagagtatt aataattttt gatactagat gatgccgaaa actaaattag
     2281 tctccggatt tactttgata gaagtcttat tggctcttac tgtcattgca attgctttaa
     2341 cagcgctttt aaaagcaacc gctcaaaata tagacaacac gcatagaatt aaagaaaaaa
     2401 caattagcca ttgggtagca atgcaagggg tagcaatgat ccaattaaat ctgctgcgca
     2461 cttctcaaag ccaagaatca acacaagcaa ccaccatgct ggggcaaaag tggtactgga
     2521 gagctaaaat tagccagacc cctataaaac gaatgcaaca gataaccatt tcagtcagtt
     2581 ccaagcaagc aggtcccttt cgagaagaac tcattgcatt caggtacttg ccatgatact
     2641 agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt gaattagatg
     2701 gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat gcaacatacg
     2761 gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca tggccaacac
     2821 ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat catatgaaac
     2881 agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga actatatttt
     2941 tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt gatacccttg
     3001 ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt cttggacaca
     3061 aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa caaaagaatg
     3121 gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt caactagcag
     3181 accattatca acaaaatact ccaattggcg atggccctgt ccttttacca gacaaccatt
     3241 acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac cacatggtcc
     3301 ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta tacaaataat
     3361 aatactagag gtcactttta cggttggcca tttgtctctt acgttgcatt tatcaatctg
     3421 ctttttgata cagcagcacc tcgctgctgc tttttttatt t
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