LOCUS BBa_M45222 5251 bp DNA linear 27-Dec-2024 DEFINITION Dual Fluorescent Color Vector ACCESSION BBa_M45222 VERSION BBa_M45222.1 FEATURES Location/Qualifiers misc_feature 1..500 /label=BBa_M45220 misc_feature 1403..1408 /label=Possible TATA Box misc_feature 623..630 /label=Possible CAAT Box promoter 509..1508 /label=BBa_M45211 misc_feature 1589..1595 /label=Possible Ribosome Binding Site misc_feature 1717..1723 /label=Possible Ribosome Binding Site (right before translation start) RBS 1517..1723 /label=BBa_M45214 misc_feature 1730..1732 /label=Start codon misc_feature 2438..2440 /label=Stop codon CDS 1730..2440 /label=BBa_M45215 misc_feature 2449..2665 /label=CaMV 35S Terminator terminator 2449..2665 /label=BBa_K1159307 misc_feature 3452..3452 /label=Changed A to T to remove EcoR1 Restriction Enzyme Site misc_feature 3270..3277 /label=Possible CAAT Box promoter 2674..3673 /label=BBa_M45212 misc_feature 3754..3760 /label=Possible Ribosome Binding Site misc_feature 3882..3888 /label=Possible Ribosome Binding Site (right before translation start) RBS 3682..3888 /label=BBa_M45214 misc_feature 3895..3897 /label=Start Codon misc_feature 4516..4518 /label=Stop Codon CDS 3895..4518 /label=BBa_M45217 misc_feature 4527..4743 /label=CaMV 35S Terminator terminator 4527..4743 /label=BBa_K1159307 misc_feature 4752..5251 /label=BBa_M45221 ORIGIN 1 atctcggatt atgggataca gcaggtaaga gacaggattc gactagaatc tctcctttgt 61 acctattgtg gttactgtcg ggatcacggg tgctgattca cattaattta ggacgggcgc 121 tgcactacgc attgttgtat gtaatgtttt tggtcttgct gattcacaca caaccttcca 181 ttggaagtcg accccttagt tatttcttat tccatttgtc actgaaaaca tttactaccc 241 tatgcaggtc aagaagatta caacagactt cgacctctga gttacagagg cgctgatgtt 301 tttcttctag cattttctct tatcagcaag gctagttatg aaaatatctc aaagaaggta 361 gttatttcct tgacgatctt gtcttcgtgt tagggttgac gacttgcttg aaaatgtttc 421 aaagtagaga accgcgtaga gggtgctatt tacgtcatat tttctgatat tgcattgcat 481 tgcagtgcat ccctgagctc tactagagga agatttgttg aagaaaagtt acatgcataa 541 tgcctcttat acatgccatg tggatatgat ttttaaataa aacaatgctt acattttaaa 601 agttgctata gaagtatatt acttcaatct ttggcccaca agcctcatga acttcaagaa 661 gcttctacat gaacttctta ttatatacta tatacaaaag taaaactaaa gccatactaa 721 atattgtagc cttgattcaa ggctctctaa tattttttgt aattgacaat tgctattgta 781 atatctttat atattatcac atatttattt atttatttat aatacttttt ctcccttctt 841 agtatgcatg tatactcttc tatagatttt gtaaatttat gtctttgata tgtaaagttg 901 taaataatac tatattgttt taacaagtat tgttaatttt tactacaatt ctaaaattct 961 acatccaaac ttaaaagaac taaacttata taaaatttaa catttaataa ttgtgttcaa 1021 atcaatgaat aaattatact ataatattaa aaaaaaatta tacaaaggta tgcatttata 1081 atattgtaat aaaaaaaatg taatcaaaat taatatataa aaaatgatat atgtgcagat 1141 ttcaaatttt ggaagtaaaa gggcgtattt ttttgataat ccggctctaa gtcacaatct 1201 ttttgctatt tgatttaact tgtccttaac tttaatgttg attttcaaaa ctctatacac 1261 atattttggc aaagtcaaga gtcttcaagt acacaacaca ttcttttccc ctaatacgtc 1321 atagtgtcag ctaattttga tccactgctt taataaaaac tcaaacatta ttcaaacaaa 1381 aaaactcaat tcttccatca aatatattca taaaactgtg ataatcctgg tttaatttat 1441 ccaatggaat gccagggtgg cgattgaaag agccccacag aaaatcacga tatttcacag 1501 gtccacccta ctagagacca tcgacagaac catatccact cgattcatgg cagcttttga 1561 cgaccatccc tagttccgat cacgcacgca caccggcttt gccacgcata ccgacttgat 1621 tgcagtcaca gcgcttgccg cgacgccaat ccgttccctt gtaccgcatc ctgacatttg 1681 tccattcgtg tttttgtttt ttgggttttg agttttgatc gagtactaga tggtatcgaa 1741 gggcgaagag gataacatgg ctataattaa ggagtttatg agattcaagg tccatatgga 1801 gggcagtgtg aatggacacg agttcgaaat tgagggggag ggtgaaggta ggccgtacga 1861 gggcactcag acagcaaagc tcaaggtgac caaaggcggt ccgctaccgt tcgcgtggga 1921 tatcctgtca ccgcagttca tgtacggttc gaaggcctac gtgaagcatc ctgccgacat 1981 acctgactac ttgaaactgt cttttcccga ggggttcaag tgggaacgtg tcatgaattt 2041 cgaagacgga ggcgtggtca cggtaactca ggattcctca ctacaagatg gcgagttcat 2101 ctataaggtg aagttaaggg ggactaattt tccatctgat gggcccgtta tgcaaaaaaa 2161 gacgatgggc tgggaggcct cgtccgagcg tatgtaccca gaggacggag ccttgaaggg 2221 tgaaattaaa cagcgcttaa aactgaagga cggggggcac tatgacgctg aagtaaagac 2281 gacttacaag gcaaagaagc ctgttcaact tcctggggcc tacaatgtga atatcaagtt 2341 agatatcaca tcgcataacg aggactatac gattgtagag caatatgaaa gagcggaagg 2401 caggcacagt actggaggca tggatgaact ttataaatag tactagagct agagtccgca 2461 aaaatcacca gtctctctct acaaatctat ctctctctat ttttctccag aataatgtgt 2521 gagtagttcc cagataaggg aattagggtt cttatagggt ttcgctcatg tgttgagcat 2581 ataagaaacc cttagtatgt atttgtattt gtaaaatact tctatcaata aaatttctaa 2641 ttcctaaaac caaaatccag tgacctacta gagtcttttt tttctttttt tttgtgtttg 2701 tttactgtta atttgcaaaa aaacgtttcc atgaaaggtt tgtaattact gatcacaact 2761 caccgattta ggagagaagc gaacattgca tccacaaatt ggagtgtttc gatgaggaac 2821 ttgagagatt catgagatgg cgcagcttgg gggagttcag gtttagggtt tgaggttcag 2881 gcctggttgt ggagatgttg cgacattaag ctcttcaatg tttttgcagg gagattcatc 2941 aagatatgtg cttctcaggg accttgctcc gtcagccaag gcggctgggt agaaatacgc 3001 cgctcctggt attgtaaaca gaaagtagat gcaactcttt gcgaattgaa tgtttgctat 3061 gccacagacc acctcgaaaa cactcggaaa aacaagcact gtattatggg acatgtcatc 3121 ttgcaacttc tcatttttct acctcaattt tggtctccca atggttctct aagcatgcat 3181 tcgtttgtaa cttgcaccga tctcaccctc catttcgatg gctggagtaa tgctcgattg 3241 tggggctaat cacgaggaaa agaacgtgtt gcaattctcc ggattccgga actgcttttg 3301 gccagctctg agggagtggc accctacagg aatcgagacc tagttttatc ttaccgtatc 3361 cgaccatatg accatcagac catggacatc tggcctgctt ccctaacccc acctgttcaa 3421 catgccacag agattttcgg taatcacggt cgtattcaat tccatctttt taaatttgtg 3481 attactctcg tgttgtgaag tgcggattcc agttggtcat tctttgcagt gcagctttaa 3541 ttgcttaatt tccctggttg ctttgaggct ggatagattg ttcgagagtc tctcgatttc 3601 agttgtctgc tgttgtggtt tctgctctaa tttttatctt catttttttt tttttttttt 3661 tttttttttt ttttactaga gaccatcgac agaaccatat ccactcgatt catggcagct 3721 tttgacgacc atccctagtt ccgatcacgc acgcacaccg gctttgccac gcataccgac 3781 ttgattgcag tcacagcgct tgccgcgacg ccaatccgtt cccttgtacc gcatcctgac 3841 atttgtccat tcgtgttttt gttttttggg ttttgagttt tgatcgagta ctagatgggc 3901 tgtatcaagt caaagagaaa ggacaatctt aatgatgacg aagctgcggg ctcaatggta 3961 tcgaaagggg aggagttatt cacaggggtc gttccgatcc tggtggagct ggacggcgat 4021 gtcaacggac ataaatttag cgtgtctgga gaaggagaag gcgatgcgac ctatggtaaa 4081 ttgactctaa agtttatttg caccacgggt aagcttcctg ttccttggcc aactctggtg 4141 actaccttcg gatacggctt gatgtgtttt gcgcgctacc cggatcacat gaagcaacat 4201 gacttcttta agagcgctat gcctgaggga tatgtccaag aacgaacaat tttttttaaa 4261 gatgatggta actataaaac cagggctgag gtgaaattcg agggggacac gcttgtgaat 4321 cgcatcgagc ttaaaggaat tgattttaag gaggacggta atattctggg acataaactg 4381 gagtacaact ataactctca taatgtgtac atcatggctg ataagcaaaa gaatggaatc 4441 aaggttaact tcaagattag gcacaacatt gaggacggaa gtgtacagct cgcagatcac 4501 taccaacaaa acacctagta ctagagctag agtccgcaaa aatcaccagt ctctctctac 4561 aaatctatct ctctctattt ttctccagaa taatgtgtga gtagttccca gataagggaa 4621 ttagggttct tatagggttt cgctcatgtg ttgagcatat aagaaaccct tagtatgtat 4681 ttgtatttgt aaaatacttc tatcaataaa atttctaatt cctaaaacca aaatccagtg 4741 acctactaga gagacactat gcgccatcgg tacccatcat tctcgttggg acgaagctag 4801 gtataaaact ccggcttcac ttgtggtttg ggaatgtgct actgacaagt ttattcaatc 4861 ggatgtaatc cacaatgctg aaaatcgaac aaaacattct agcacggatg cgctgtgctc 4921 ttgcttgtca tttttattct caagtatttt tcactctctt acagctggcg cgtcaattgg 4981 ctgatcttcg agatgataaa cagttctttg ctgaccatcc tggagcggct ccaattacaa 5041 cctctcaagt aagttgaagt atgcatgcat tgctcccaaa tttaacgtaa tgcaagtagt 5101 ctttcaaaga agcccttgag ttatcggaaa gttaagatgt tgtctttggt agccagtcat 5161 gagtactcca attgatggcc ctcgtacaca attttcagct atggcctagc ctgtgctgac 5221 tggagctctc gtctggatca cgtgttgtca c //