BBa_M45214 1 BBa_M45214 Physcomitrella patens ribosome binding site 2016-04-15T11:00:00Z 2016-04-16T01:01:24Z From genbank: Physcomitrella patens subsp. patens predicted protein (PHYPADRAFT_173331) mRNA, complete cds. This sequence is the DNA sequence between the transcription start and translation start sites on a dehydrin protein gene found in P. patens. While it is true that this entire sequence may not be involved in ribosome binding, it is clear that this sequence contains key elements for ribosome binding and even more specifically a ribosome binding site that is native to P. patens. false false _1855_ 23449 23449 9 false Only the sequence between the transcription and translation start sites were taken into consideration. false Cody Maughan annotation2478749 1 Possible Ribosome Binding Site (right before translation start) range2478749 1 201 207 annotation2478748 1 Possible Ribosome Binding Site range2478748 1 73 79 BBa_K1159307 1 BBa_K1159307 35S Terminator of Cauliflower Mosaic Virus (CaMV) 2013-09-13T11:00:00Z 2015-06-11T01:46:04Z Cauliflower Mosaic Virus This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix. false false _1471_ 4206 11507 9 In stock false x false TU Munich 2013 annotation2341614 1 CaMV 35S Terminator range2341614 1 1 217 BBa_M45217 1 BBa_M45217 mCitrine 2016-04-15T11:00:00Z 2016-04-16T03:18:09Z Synthetic construct Fluorescent protein in Yellow-Green spectrum false false _1855_ 30475 30475 9 false None false Iegor Pererva annotation2478762 1 Start Codon range2478762 1 1 3 annotation2478763 1 Stop Codon range2478763 1 622 624 BBa_M45211 1 BBa_M45211 Physcomitrella patens dehydrin promoter 2 2016-04-10T11:00:00Z 2016-04-11T10:57:35Z Zolotarov, Y. and Str??mvik, M. (2015). De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes. PLoS One, 10 (6). This DNA sequence is a native promoter region in Physcomitrella patens for a dehydrin protein. Dehydrin proteins are produced when drought stress or salt stress occurs within an organism. The exact mechanism of this promoter is not known, however it has been validated in the literature as a promoter region for a dehydrin protein in Physcomitrella patens. false false _1855_ 23449 23449 9 false The promoter was assumed to be the 1000 bp directly before the transcription start site of the dehydrin gene. false Cody Maughan annotation2478743 1 Possible CAAT Box range2478743 1 115 122 annotation2478742 1 Possible TATA Box range2478742 1 895 900 BBa_M45222 1 BBa_M45222 Dual Fluorescent Color Vector 2016-04-24T11:00:00Z 2016-04-25T05:25:42Z Physcomitrella patens, mixed synthetic constructs Vector to transfect Physcomitrella patens with 2 fluorescent proteins [mCherry and mCitrine] activated by dry-shock, through homologous recombination. false false _1855_ 30477 30477 9 false none false Justin Marriott component2479231 1 BBa_M45214 component2479236 1 BBa_K1159307 component2479248 1 BBa_M45221 component2479239 1 BBa_M45212 component2479234 1 BBa_M45215 component2479228 1 BBa_M45211 component2479242 1 BBa_M45214 component2479247 1 BBa_K1159307 component2479245 1 BBa_M45217 component2479225 1 BBa_M45220 annotation2479231 1 BBa_M45214 range2479231 1 1517 1723 annotation2479236 1 BBa_K1159307 range2479236 1 2449 2665 annotation2479234 1 BBa_M45215 range2479234 1 1730 2440 annotation2479245 1 BBa_M45217 range2479245 1 3895 4518 annotation2479248 1 BBa_M45221 range2479248 1 4752 5251 annotation2479225 1 BBa_M45220 range2479225 1 1 500 annotation2479242 1 BBa_M45214 range2479242 1 3682 3888 annotation2479247 1 BBa_K1159307 range2479247 1 4527 4743 annotation2479239 1 BBa_M45212 range2479239 1 2674 3673 annotation2479228 1 BBa_M45211 range2479228 1 509 1508 BBa_M45215 1 BBa_M45215 mCherry 2016-04-15T11:00:00Z 2016-04-16T02:20:17Z Synthetic construct mCherry - fluorescent protein in RED specturm false false _1855_ 30475 30475 9 false None false Iegor Pererva annotation2478752 1 Start codon range2478752 1 1 3 annotation2478753 1 Stop codon range2478753 1 709 711 BBa_M45212 1 BBa_M45212 Physcomitrella patens dehydrin promoter 3 2016-04-10T11:00:00Z 2016-04-16T12:28:31Z Zolotarov, Y. and Str??mvik, M. (2015). De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes. PLoS One, 10 (6). This DNA sequence is a native promoter region in Physcomitrella patens for a dehydrin protein. Dehydrin proteins are produced when drought stress or salt stress occurs within an organism. The exact mechanism of this promoter is not known, however it has been validated in the literature as a promoter region for a dehydrin protein in Physcomitrella patens. false false _1855_ 23449 23449 9 false The promoter was assumed to be the 1000 bp directly before the transcription start site of the dehydrin gene. false Cody Maughan annotation2478744 1 Changed A to T to remove EcoR1 Restriction Enzyme Site range2478744 1 779 779 annotation2478745 1 Possible CAAT Box range2478745 1 597 604 BBa_M45220 1 BBa_M45220 pre-homologous PpRac-1 section 2016-04-24T11:00:00Z 2016-04-25T05:27:50Z Physcomitrella patens A homologous, though incomplete, section of the PpRac-1 gene to be used as the 5' boundary of our vector to facilitate homologous recombination in Physcomitrella patens. false false _1855_ 30477 30477 9 false none false Justin Marriott BBa_M45221 1 BBa_M45221 post-homologous PpRac-1 section 2016-04-24T11:00:00Z 2016-04-25T05:28:16Z Physcomitrella patens A homologous, though incomplete, section of the PpRac-1 gene to be used as the 3' boundary of our vector to facilitate homologous recombination in Physcomitrella patens. false false _1855_ 30477 30477 9 false none false Justin Marriott BBa_M45217_sequence 1 atgggctgtatcaagtcaaagagaaaggacaatcttaatgatgacgaagctgcgggctcaatggtatcgaaaggggaggagttattcacaggggtcgttccgatcctggtggagctggacggcgatgtcaacggacataaatttagcgtgtctggagaaggagaaggcgatgcgacctatggtaaattgactctaaagtttatttgcaccacgggtaagcttcctgttccttggccaactctggtgactaccttcggatacggcttgatgtgttttgcgcgctacccggatcacatgaagcaacatgacttctttaagagcgctatgcctgagggatatgtccaagaacgaacaattttttttaaagatgatggtaactataaaaccagggctgaggtgaaattcgagggggacacgcttgtgaatcgcatcgagcttaaaggaattgattttaaggaggacggtaatattctgggacataaactggagtacaactataactctcataatgtgtacatcatggctgataagcaaaagaatggaatcaaggttaacttcaagattaggcacaacattgaggacggaagtgtacagctcgcagatcactaccaacaaaacacctag BBa_M45214_sequence 1 accatcgacagaaccatatccactcgattcatggcagcttttgacgaccatccctagttccgatcacgcacgcacaccggctttgccacgcataccgacttgattgcagtcacagcgcttgccgcgacgccaatccgttcccttgtaccgcatcctgacatttgtccattcgtgtttttgttttttgggttttgagttttgatcgag BBa_M45215_sequence 1 atggtatcgaagggcgaagaggataacatggctataattaaggagtttatgagattcaaggtccatatggagggcagtgtgaatggacacgagttcgaaattgagggggagggtgaaggtaggccgtacgagggcactcagacagcaaagctcaaggtgaccaaaggcggtccgctaccgttcgcgtgggatatcctgtcaccgcagttcatgtacggttcgaaggcctacgtgaagcatcctgccgacatacctgactacttgaaactgtcttttcccgaggggttcaagtgggaacgtgtcatgaatttcgaagacggaggcgtggtcacggtaactcaggattcctcactacaagatggcgagttcatctataaggtgaagttaagggggactaattttccatctgatgggcccgttatgcaaaaaaagacgatgggctgggaggcctcgtccgagcgtatgtacccagaggacggagccttgaagggtgaaattaaacagcgcttaaaactgaaggacggggggcactatgacgctgaagtaaagacgacttacaaggcaaagaagcctgttcaacttcctggggcctacaatgtgaatatcaagttagatatcacatcgcataacgaggactatacgattgtagagcaatatgaaagagcggaaggcaggcacagtactggaggcatggatgaactttataaatag BBa_M45222_sequence 1 atctcggattatgggatacagcaggtaagagacaggattcgactagaatctctcctttgtacctattgtggttactgtcgggatcacgggtgctgattcacattaatttaggacgggcgctgcactacgcattgttgtatgtaatgtttttggtcttgctgattcacacacaaccttccattggaagtcgaccccttagttatttcttattccatttgtcactgaaaacatttactaccctatgcaggtcaagaagattacaacagacttcgacctctgagttacagaggcgctgatgtttttcttctagcattttctcttatcagcaaggctagttatgaaaatatctcaaagaaggtagttatttccttgacgatcttgtcttcgtgttagggttgacgacttgcttgaaaatgtttcaaagtagagaaccgcgtagagggtgctatttacgtcatattttctgatattgcattgcattgcagtgcatccctgagctctactagaggaagatttgttgaagaaaagttacatgcataatgcctcttatacatgccatgtggatatgatttttaaataaaacaatgcttacattttaaaagttgctatagaagtatattacttcaatctttggcccacaagcctcatgaacttcaagaagcttctacatgaacttcttattatatactatatacaaaagtaaaactaaagccatactaaatattgtagccttgattcaaggctctctaatattttttgtaattgacaattgctattgtaatatctttatatattatcacatatttatttatttatttataatactttttctcccttcttagtatgcatgtatactcttctatagattttgtaaatttatgtctttgatatgtaaagttgtaaataatactatattgttttaacaagtattgttaatttttactacaattctaaaattctacatccaaacttaaaagaactaaacttatataaaatttaacatttaataattgtgttcaaatcaatgaataaattatactataatattaaaaaaaaattatacaaaggtatgcatttataatattgtaataaaaaaaatgtaatcaaaattaatatataaaaaatgatatatgtgcagatttcaaattttggaagtaaaagggcgtatttttttgataatccggctctaagtcacaatctttttgctatttgatttaacttgtccttaactttaatgttgattttcaaaactctatacacatattttggcaaagtcaagagtcttcaagtacacaacacattcttttcccctaatacgtcatagtgtcagctaattttgatccactgctttaataaaaactcaaacattattcaaacaaaaaaactcaattcttccatcaaatatattcataaaactgtgataatcctggtttaatttatccaatggaatgccagggtggcgattgaaagagccccacagaaaatcacgatatttcacaggtccaccctactagagaccatcgacagaaccatatccactcgattcatggcagcttttgacgaccatccctagttccgatcacgcacgcacaccggctttgccacgcataccgacttgattgcagtcacagcgcttgccgcgacgccaatccgttcccttgtaccgcatcctgacatttgtccattcgtgtttttgttttttgggttttgagttttgatcgagtactagatggtatcgaagggcgaagaggataacatggctataattaaggagtttatgagattcaaggtccatatggagggcagtgtgaatggacacgagttcgaaattgagggggagggtgaaggtaggccgtacgagggcactcagacagcaaagctcaaggtgaccaaaggcggtccgctaccgttcgcgtgggatatcctgtcaccgcagttcatgtacggttcgaaggcctacgtgaagcatcctgccgacatacctgactacttgaaactgtcttttcccgaggggttcaagtgggaacgtgtcatgaatttcgaagacggaggcgtggtcacggtaactcaggattcctcactacaagatggcgagttcatctataaggtgaagttaagggggactaattttccatctgatgggcccgttatgcaaaaaaagacgatgggctgggaggcctcgtccgagcgtatgtacccagaggacggagccttgaagggtgaaattaaacagcgcttaaaactgaaggacggggggcactatgacgctgaagtaaagacgacttacaaggcaaagaagcctgttcaacttcctggggcctacaatgtgaatatcaagttagatatcacatcgcataacgaggactatacgattgtagagcaatatgaaagagcggaaggcaggcacagtactggaggcatggatgaactttataaatagtactagagctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacctactagagtcttttttttctttttttttgtgtttgtttactgttaatttgcaaaaaaacgtttccatgaaaggtttgtaattactgatcacaactcaccgatttaggagagaagcgaacattgcatccacaaattggagtgtttcgatgaggaacttgagagattcatgagatggcgcagcttgggggagttcaggtttagggtttgaggttcaggcctggttgtggagatgttgcgacattaagctcttcaatgtttttgcagggagattcatcaagatatgtgcttctcagggaccttgctccgtcagccaaggcggctgggtagaaatacgccgctcctggtattgtaaacagaaagtagatgcaactctttgcgaattgaatgtttgctatgccacagaccacctcgaaaacactcggaaaaacaagcactgtattatgggacatgtcatcttgcaacttctcatttttctacctcaattttggtctcccaatggttctctaagcatgcattcgtttgtaacttgcaccgatctcaccctccatttcgatggctggagtaatgctcgattgtggggctaatcacgaggaaaagaacgtgttgcaattctccggattccggaactgcttttggccagctctgagggagtggcaccctacaggaatcgagacctagttttatcttaccgtatccgaccatatgaccatcagaccatggacatctggcctgcttccctaaccccacctgttcaacatgccacagagattttcggtaatcacggtcgtattcaattccatctttttaaatttgtgattactctcgtgttgtgaagtgcggattccagttggtcattctttgcagtgcagctttaattgcttaatttccctggttgctttgaggctggatagattgttcgagagtctctcgatttcagttgtctgctgttgtggtttctgctctaatttttatcttcattttttttttttttttttttttttttttttttactagagaccatcgacagaaccatatccactcgattcatggcagcttttgacgaccatccctagttccgatcacgcacgcacaccggctttgccacgcataccgacttgattgcagtcacagcgcttgccgcgacgccaatccgttcccttgtaccgcatcctgacatttgtccattcgtgtttttgttttttgggttttgagttttgatcgagtactagatgggctgtatcaagtcaaagagaaaggacaatcttaatgatgacgaagctgcgggctcaatggtatcgaaaggggaggagttattcacaggggtcgttccgatcctggtggagctggacggcgatgtcaacggacataaatttagcgtgtctggagaaggagaaggcgatgcgacctatggtaaattgactctaaagtttatttgcaccacgggtaagcttcctgttccttggccaactctggtgactaccttcggatacggcttgatgtgttttgcgcgctacccggatcacatgaagcaacatgacttctttaagagcgctatgcctgagggatatgtccaagaacgaacaattttttttaaagatgatggtaactataaaaccagggctgaggtgaaattcgagggggacacgcttgtgaatcgcatcgagcttaaaggaattgattttaaggaggacggtaatattctgggacataaactggagtacaactataactctcataatgtgtacatcatggctgataagcaaaagaatggaatcaaggttaacttcaagattaggcacaacattgaggacggaagtgtacagctcgcagatcactaccaacaaaacacctagtactagagctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacctactagagagacactatgcgccatcggtacccatcattctcgttgggacgaagctaggtataaaactccggcttcacttgtggtttgggaatgtgctactgacaagtttattcaatcggatgtaatccacaatgctgaaaatcgaacaaaacattctagcacggatgcgctgtgctcttgcttgtcatttttattctcaagtatttttcactctcttacagctggcgcgtcaattggctgatcttcgagatgataaacagttctttgctgaccatcctggagcggctccaattacaacctctcaagtaagttgaagtatgcatgcattgctcccaaatttaacgtaatgcaagtagtctttcaaagaagcccttgagttatcggaaagttaagatgttgtctttggtagccagtcatgagtactccaattgatggccctcgtacacaattttcagctatggcctagcctgtgctgactggagctctcgtctggatcacgtgttgtcac BBa_M45212_sequence 1 tcttttttttctttttttttgtgtttgtttactgttaatttgcaaaaaaacgtttccatgaaaggtttgtaattactgatcacaactcaccgatttaggagagaagcgaacattgcatccacaaattggagtgtttcgatgaggaacttgagagattcatgagatggcgcagcttgggggagttcaggtttagggtttgaggttcaggcctggttgtggagatgttgcgacattaagctcttcaatgtttttgcagggagattcatcaagatatgtgcttctcagggaccttgctccgtcagccaaggcggctgggtagaaatacgccgctcctggtattgtaaacagaaagtagatgcaactctttgcgaattgaatgtttgctatgccacagaccacctcgaaaacactcggaaaaacaagcactgtattatgggacatgtcatcttgcaacttctcatttttctacctcaattttggtctcccaatggttctctaagcatgcattcgtttgtaacttgcaccgatctcaccctccatttcgatggctggagtaatgctcgattgtggggctaatcacgaggaaaagaacgtgttgcaattctccggattccggaactgcttttggccagctctgagggagtggcaccctacaggaatcgagacctagttttatcttaccgtatccgaccatatgaccatcagaccatggacatctggcctgcttccctaaccccacctgttcaacatgccacagagattttcggtaatcacggtcgtattcaattccatctttttaaatttgtgattactctcgtgttgtgaagtgcggattccagttggtcattctttgcagtgcagctttaattgcttaatttccctggttgctttgaggctggatagattgttcgagagtctctcgatttcagttgtctgctgttgtggtttctgctctaatttttatcttcattttttttttttttttttttttttttttttt BBa_K1159307_sequence 1 ctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacc BBa_M45221_sequence 1 agacactatgcgccatcggtacccatcattctcgttgggacgaagctaggtataaaactccggcttcacttgtggtttgggaatgtgctactgacaagtttattcaatcggatgtaatccacaatgctgaaaatcgaacaaaacattctagcacggatgcgctgtgctcttgcttgtcatttttattctcaagtatttttcactctcttacagctggcgcgtcaattggctgatcttcgagatgataaacagttctttgctgaccatcctggagcggctccaattacaacctctcaagtaagttgaagtatgcatgcattgctcccaaatttaacgtaatgcaagtagtctttcaaagaagcccttgagttatcggaaagttaagatgttgtctttggtagccagtcatgagtactccaattgatggccctcgtacacaattttcagctatggcctagcctgtgctgactggagctctcgtctggatcacgtgttgtcac BBa_M45211_sequence 1 gaagatttgttgaagaaaagttacatgcataatgcctcttatacatgccatgtggatatgatttttaaataaaacaatgcttacattttaaaagttgctatagaagtatattacttcaatctttggcccacaagcctcatgaacttcaagaagcttctacatgaacttcttattatatactatatacaaaagtaaaactaaagccatactaaatattgtagccttgattcaaggctctctaatattttttgtaattgacaattgctattgtaatatctttatatattatcacatatttatttatttatttataatactttttctcccttcttagtatgcatgtatactcttctatagattttgtaaatttatgtctttgatatgtaaagttgtaaataatactatattgttttaacaagtattgttaatttttactacaattctaaaattctacatccaaacttaaaagaactaaacttatataaaatttaacatttaataattgtgttcaaatcaatgaataaattatactataatattaaaaaaaaattatacaaaggtatgcatttataatattgtaataaaaaaaatgtaatcaaaattaatatataaaaaatgatatatgtgcagatttcaaattttggaagtaaaagggcgtatttttttgataatccggctctaagtcacaatctttttgctatttgatttaacttgtccttaactttaatgttgattttcaaaactctatacacatattttggcaaagtcaagagtcttcaagtacacaacacattcttttcccctaatacgtcatagtgtcagctaattttgatccactgctttaataaaaactcaaacattattcaaacaaaaaaactcaattcttccatcaaatatattcataaaactgtgataatcctggtttaatttatccaatggaatgccagggtggcgattgaaagagccccacagaaaatcacgatatttcacaggtccaccc BBa_M45220_sequence 1 atctcggattatgggatacagcaggtaagagacaggattcgactagaatctctcctttgtacctattgtggttactgtcgggatcacgggtgctgattcacattaatttaggacgggcgctgcactacgcattgttgtatgtaatgtttttggtcttgctgattcacacacaaccttccattggaagtcgaccccttagttatttcttattccatttgtcactgaaaacatttactaccctatgcaggtcaagaagattacaacagacttcgacctctgagttacagaggcgctgatgtttttcttctagcattttctcttatcagcaaggctagttatgaaaatatctcaaagaaggtagttatttccttgacgatcttgtcttcgtgttagggttgacgacttgcttgaaaatgtttcaaagtagagaaccgcgtagagggtgctatttacgtcatattttctgatattgcattgcattgcagtgcatccctgagctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z