Physcomitrella patens, mixed synthetic constructs
Vector to transfect Physcomitrella patens with 2 fluorescent proteins [mCherry and mCitrine] activated by dry-shock, through homologous recombination.
Physcomitrella patens dehydrin promoter 2
Zolotarov, Y. and Str??mvik, M. (2015). De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes. PLoS One, 10 (6).
This DNA sequence is a native promoter region in Physcomitrella patens for a dehydrin protein. Dehydrin proteins are produced when drought stress or salt stress occurs within an organism. The exact mechanism of this promoter is not known, however it has been validated in the literature as a promoter region for a dehydrin protein in Physcomitrella patens.
The promoter was assumed to be the 1000 bp directly before the transcription start site of the dehydrin gene.
Physcomitrella patens dehydrin promoter 3
Changed A to T to remove EcoR1 Restriction Enzyme Site
Physcomitrella patens ribosome binding site
From genbank: Physcomitrella patens subsp. patens predicted protein (PHYPADRAFT_173331) mRNA, complete cds.
This sequence is the DNA sequence between the transcription start and translation start sites on a dehydrin protein gene found in P. patens. While it is true that this entire sequence may not be involved in ribosome binding, it is clear that this sequence contains key elements for ribosome binding and even more specifically a ribosome binding site that is native to P. patens.
Only the sequence between the transcription and translation start sites were taken into consideration.
Possible Ribosome Binding Site (right before translation start)
mCherry - fluorescent protein in RED specturm
Fluorescent protein in Yellow-Green spectrum
A homologous, though incomplete, section of the PpRac-1 gene to be used as the 5' boundary of our vector to facilitate homologous recombination in Physcomitrella patens.
A homologous, though incomplete, section of the PpRac-1 gene to be used as the 3' boundary of our vector to facilitate homologous recombination in Physcomitrella patens.
35S Terminator of Cauliflower Mosaic Virus (CaMV)
This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix.
Conversion of the iGEM parts registry to SBOL2.1