BBa_M45222
type: ComponentDefinition
persistent identity: BBa M45222
display id: BBa_M45222
version: 1
was generated by: iGEM to SBOL conversion
description: Dual Fluorescent Color Vector
created: 2016-04-24T11:00:00Z
modified: 2016-04-25T05:25:42Z
mutable provenance: Physcomitrella patens, mixed synthetic constructs
owned by: myers
owned by: james
mutable description: Vector to transfect Physcomitrella patens with 2 fluorescent proteins [mCherry and mCitrine] activated by dry-shock, through homologous recombination.
discontinued: false
dominant: false
group u list: _1855_
m user id: 30477
owner id: 30477
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Justin Marriott
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M45221
component: BBa_K1159307
component: BBa_M45215
component: BBa_M45214
component: BBa_M45211
component: BBa_M45217
component: BBa_M45212
component: BBa_M45220
sequence annotation: BBa_M45220
sequence annotation: BBa_M45211
sequence annotation: BBa_M45214
sequence annotation: BBa_M45215
sequence annotation: BBa_K1159307
sequence annotation: BBa_M45212
sequence annotation: BBa_M45217
sequence annotation: BBa_M45221
sequence: https://synbiohub.org/public/igem/BBa_M45222_sequence/1

BBa_M45221
type: Component
persistent identity: component2479248
display id: component2479248
version: 1
top level: BBa_M45222
definition: BBa_M45221
access: public

BBa_K1159307
type: Component
persistent identity: component2479236
display id: component2479236
version: 1
top level: BBa_M45222
definition: BBa_K1159307
access: public

BBa_M45215
type: Component
persistent identity: component2479234
display id: component2479234
version: 1
top level: BBa_M45222
definition: BBa_M45215
access: public

BBa_M45214
type: Component
persistent identity: component2479231
display id: component2479231
version: 1
top level: BBa_M45222
definition: BBa_M45214
access: public

BBa_M45211
type: Component
persistent identity: component2479228
display id: component2479228
version: 1
top level: BBa_M45222
definition: BBa_M45211
access: public

BBa_K1159307
type: Component
persistent identity: component2479247
display id: component2479247
version: 1
top level: BBa_M45222
definition: BBa_K1159307
access: public

BBa_M45217
type: Component
persistent identity: component2479245
display id: component2479245
version: 1
top level: BBa_M45222
definition: BBa_M45217
access: public

BBa_M45214
type: Component
persistent identity: component2479242
display id: component2479242
version: 1
top level: BBa_M45222
definition: BBa_M45214
access: public

BBa_M45212
type: Component
persistent identity: component2479239
display id: component2479239
version: 1
top level: BBa_M45222
definition: BBa_M45212
access: public

BBa_M45220
type: Component
persistent identity: component2479225
display id: component2479225
version: 1
top level: BBa_M45222
definition: BBa_M45220
access: public

BBa_M45220
type: SequenceAnnotation
persistent identity: annotation2479225
display id: annotation2479225
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479225/range2479225/1
component: BBa_M45220

https://synbiohub.org/public/igem/BBa_M45222/annotation2479225/range2479225/1
type: Range
persistent identity: range2479225
display id: range2479225
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 1
end: 500
orientation: inline

BBa_M45211
type: SequenceAnnotation
persistent identity: annotation2479228
display id: annotation2479228
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479228/range2479228/1
component: BBa_M45211

https://synbiohub.org/public/igem/BBa_M45222/annotation2479228/range2479228/1
type: Range
persistent identity: range2479228
display id: range2479228
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 509
end: 1508
orientation: inline

BBa_M45214
type: SequenceAnnotation
persistent identity: annotation2479231
display id: annotation2479231
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479231/range2479231/1
component: BBa_M45214

https://synbiohub.org/public/igem/BBa_M45222/annotation2479231/range2479231/1
type: Range
persistent identity: range2479231
display id: range2479231
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 1517
end: 1723
orientation: inline

BBa_M45215
type: SequenceAnnotation
persistent identity: annotation2479234
display id: annotation2479234
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479234/range2479234/1
component: BBa_M45215

https://synbiohub.org/public/igem/BBa_M45222/annotation2479234/range2479234/1
type: Range
persistent identity: range2479234
display id: range2479234
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 1730
end: 2440
orientation: inline

BBa_K1159307
type: SequenceAnnotation
persistent identity: annotation2479236
display id: annotation2479236
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479236/range2479236/1
component: BBa_K1159307

https://synbiohub.org/public/igem/BBa_M45222/annotation2479236/range2479236/1
type: Range
persistent identity: range2479236
display id: range2479236
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 2449
end: 2665
orientation: inline

BBa_M45212
type: SequenceAnnotation
persistent identity: annotation2479239
display id: annotation2479239
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479239/range2479239/1
component: BBa_M45212

https://synbiohub.org/public/igem/BBa_M45222/annotation2479239/range2479239/1
type: Range
persistent identity: range2479239
display id: range2479239
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 2674
end: 3673
orientation: inline

BBa_M45214
type: SequenceAnnotation
persistent identity: annotation2479242
display id: annotation2479242
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479242/range2479242/1
component: BBa_M45214

https://synbiohub.org/public/igem/BBa_M45222/annotation2479242/range2479242/1
type: Range
persistent identity: range2479242
display id: range2479242
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 3682
end: 3888
orientation: inline

BBa_M45217
type: SequenceAnnotation
persistent identity: annotation2479245
display id: annotation2479245
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479245/range2479245/1
component: BBa_M45217

https://synbiohub.org/public/igem/BBa_M45222/annotation2479245/range2479245/1
type: Range
persistent identity: range2479245
display id: range2479245
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 3895
end: 4518
orientation: inline

BBa_K1159307
type: SequenceAnnotation
persistent identity: annotation2479247
display id: annotation2479247
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479247/range2479247/1
component: BBa_K1159307

https://synbiohub.org/public/igem/BBa_M45222/annotation2479247/range2479247/1
type: Range
persistent identity: range2479247
display id: range2479247
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 4527
end: 4743
orientation: inline

BBa_M45221
type: SequenceAnnotation
persistent identity: annotation2479248
display id: annotation2479248
version: 1
top level: BBa_M45222
location: https://synbiohub.org/public/igem/BBa_M45222/annotation2479248/range2479248/1
component: BBa_M45221

https://synbiohub.org/public/igem/BBa_M45222/annotation2479248/range2479248/1
type: Range
persistent identity: range2479248
display id: range2479248
version: 1
top level: BBa_M45222
direction: direction/reverse
start: 4752
end: 5251
orientation: inline

BBa_M45211
type: ComponentDefinition
persistent identity: BBa M45211
display id: BBa_M45211
version: 1
was generated by: iGEM to SBOL conversion
description: Physcomitrella patens dehydrin promoter 2
created: 2016-04-10T11:00:00Z
modified: 2016-04-11T10:57:35Z
mutable provenance: Zolotarov, Y. and Str??mvik, M. (2015). De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes. PLoS One, 10 (6).
owned by: james
owned by: myers
mutable description: This DNA sequence is a native promoter region in Physcomitrella patens for a dehydrin protein. Dehydrin proteins are produced when drought stress or salt stress occurs within an organism. The exact mechanism of this promoter is not known, however it has been validated in the literature as a promoter region for a dehydrin protein in Physcomitrella patens.
discontinued: false
dominant: false
group u list: _1855_
m user id: 23449
owner id: 23449
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The promoter was assumed to be the 1000 bp directly before the transcription start site of the dehydrin gene.
star: false
creator: Cody Maughan
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Possible CAAT Box
sequence annotation: Possible TATA Box
sequence: https://synbiohub.org/public/igem/BBa_M45211_sequence/1

Possible CAAT Box
type: SequenceAnnotation
persistent identity: annotation2478743
display id: annotation2478743
version: 1
top level: BBa_M45211
location: https://synbiohub.org/public/igem/BBa_M45211/annotation2478743/range2478743/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_M45211/annotation2478743/range2478743/1
type: Range
persistent identity: range2478743
display id: range2478743
version: 1
top level: BBa_M45211
direction: direction/forward
start: 115
end: 122
orientation: inline

Possible TATA Box
type: SequenceAnnotation
persistent identity: annotation2478742
display id: annotation2478742
version: 1
top level: BBa_M45211
location: https://synbiohub.org/public/igem/BBa_M45211/annotation2478742/range2478742/1
role: feature/conserved
role: SO:0000330

https://synbiohub.org/public/igem/BBa_M45211/annotation2478742/range2478742/1
type: Range
persistent identity: range2478742
display id: range2478742
version: 1
top level: BBa_M45211
direction: direction/forward
start: 895
end: 900
orientation: inline

BBa_M45212
type: ComponentDefinition
persistent identity: BBa M45212
display id: BBa_M45212
version: 1
was generated by: iGEM to SBOL conversion
description: Physcomitrella patens dehydrin promoter 3
created: 2016-04-10T11:00:00Z
modified: 2016-04-16T12:28:31Z
mutable provenance: Zolotarov, Y. and Str??mvik, M. (2015). De Novo Regulatory Motif Discovery Identifies Significant Motifs in Promoters of Five Classes of Plant Dehydrin Genes. PLoS One, 10 (6).
owned by: james
owned by: myers
mutable description: This DNA sequence is a native promoter region in Physcomitrella patens for a dehydrin protein. Dehydrin proteins are produced when drought stress or salt stress occurs within an organism. The exact mechanism of this promoter is not known, however it has been validated in the literature as a promoter region for a dehydrin protein in Physcomitrella patens.
discontinued: false
dominant: false
group u list: _1855_
m user id: 23449
owner id: 23449
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: The promoter was assumed to be the 1000 bp directly before the transcription start site of the dehydrin gene.
star: false
creator: Cody Maughan
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence annotation: Possible CAAT Box
sequence annotation: Changed A to T to remove EcoR1 Restriction Enzyme Site
sequence: https://synbiohub.org/public/igem/BBa_M45212_sequence/1

Possible CAAT Box
type: SequenceAnnotation
persistent identity: annotation2478745
display id: annotation2478745
version: 1
top level: BBa_M45212
location: https://synbiohub.org/public/igem/BBa_M45212/annotation2478745/range2478745/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_M45212/annotation2478745/range2478745/1
type: Range
persistent identity: range2478745
display id: range2478745
version: 1
top level: BBa_M45212
direction: direction/forward
start: 597
end: 604
orientation: inline

Changed A to T to remove EcoR1 Restriction Enzyme Site
type: SequenceAnnotation
persistent identity: annotation2478744
display id: annotation2478744
version: 1
top level: BBa_M45212
location: https://synbiohub.org/public/igem/BBa_M45212/annotation2478744/range2478744/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_M45212/annotation2478744/range2478744/1
type: Range
persistent identity: range2478744
display id: range2478744
version: 1
top level: BBa_M45212
direction: direction/forward
start: 779
end: 779
orientation: inline

BBa_M45214
type: ComponentDefinition
persistent identity: BBa M45214
display id: BBa_M45214
version: 1
was generated by: iGEM to SBOL conversion
description: Physcomitrella patens ribosome binding site
created: 2016-04-15T11:00:00Z
modified: 2016-04-16T01:01:24Z
mutable provenance: From genbank: Physcomitrella patens subsp. patens predicted protein (PHYPADRAFT_173331) mRNA, complete cds.
owned by: james
owned by: myers
mutable description: This sequence is the DNA sequence between the transcription start and translation start sites on a dehydrin protein gene found in P. patens. While it is true that this entire sequence may not be involved in ribosome binding, it is clear that this sequence contains key elements for ribosome binding and even more specifically a ribosome binding site that is native to P. patens.
discontinued: false
dominant: false
group u list: _1855_
m user id: 23449
owner id: 23449
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: Only the sequence between the transcription and translation start sites were taken into consideration.
star: false
creator: Cody Maughan
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: Possible Ribosome Binding Site
sequence annotation: Possible Ribosome Binding Site (right before translation start)
sequence: https://synbiohub.org/public/igem/BBa_M45214_sequence/1

Possible Ribosome Binding Site
type: SequenceAnnotation
persistent identity: annotation2478748
display id: annotation2478748
version: 1
top level: BBa_M45214
location: https://synbiohub.org/public/igem/BBa_M45214/annotation2478748/range2478748/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M45214/annotation2478748/range2478748/1
type: Range
persistent identity: range2478748
display id: range2478748
version: 1
top level: BBa_M45214
direction: direction/forward
start: 73
end: 79
orientation: inline

Possible Ribosome Binding Site (right before translation start)
type: SequenceAnnotation
persistent identity: annotation2478749
display id: annotation2478749
version: 1
top level: BBa_M45214
location: https://synbiohub.org/public/igem/BBa_M45214/annotation2478749/range2478749/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_M45214/annotation2478749/range2478749/1
type: Range
persistent identity: range2478749
display id: range2478749
version: 1
top level: BBa_M45214
direction: direction/forward
start: 201
end: 207
orientation: inline

BBa_M45215
type: ComponentDefinition
persistent identity: BBa M45215
display id: BBa_M45215
version: 1
was generated by: iGEM to SBOL conversion
description: mCherry
created: 2016-04-15T11:00:00Z
modified: 2016-04-16T02:20:17Z
mutable provenance: Synthetic construct
owned by: james
owned by: myers
mutable description: mCherry - fluorescent protein in RED specturm
discontinued: false
dominant: false
group u list: _1855_
m user id: 30475
owner id: 30475
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Iegor Pererva
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: Start codon
sequence annotation: Stop codon
sequence: https://synbiohub.org/public/igem/BBa_M45215_sequence/1

Start codon
type: SequenceAnnotation
persistent identity: annotation2478752
display id: annotation2478752
version: 1
top level: BBa_M45215
location: https://synbiohub.org/public/igem/BBa_M45215/annotation2478752/range2478752/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M45215/annotation2478752/range2478752/1
type: Range
persistent identity: range2478752
display id: range2478752
version: 1
top level: BBa_M45215
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop codon
type: SequenceAnnotation
persistent identity: annotation2478753
display id: annotation2478753
version: 1
top level: BBa_M45215
location: https://synbiohub.org/public/igem/BBa_M45215/annotation2478753/range2478753/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_M45215/annotation2478753/range2478753/1
type: Range
persistent identity: range2478753
display id: range2478753
version: 1
top level: BBa_M45215
direction: direction/forward
start: 709
end: 711
orientation: inline

BBa_M45217
type: ComponentDefinition
persistent identity: BBa M45217
display id: BBa_M45217
version: 1
was generated by: iGEM to SBOL conversion
description: mCitrine
created: 2016-04-15T11:00:00Z
modified: 2016-04-16T03:18:09Z
mutable provenance: Synthetic construct
owned by: james
owned by: myers
mutable description: Fluorescent protein in Yellow-Green spectrum
discontinued: false
dominant: false
group u list: _1855_
m user id: 30475
owner id: 30475
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: None
star: false
creator: Iegor Pererva
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: Start Codon
sequence annotation: Stop Codon
sequence: https://synbiohub.org/public/igem/BBa_M45217_sequence/1

Start Codon
type: SequenceAnnotation
persistent identity: annotation2478762
display id: annotation2478762
version: 1
top level: BBa_M45217
location: https://synbiohub.org/public/igem/BBa_M45217/annotation2478762/range2478762/1
role: SO:0000318
role: feature/start

https://synbiohub.org/public/igem/BBa_M45217/annotation2478762/range2478762/1
type: Range
persistent identity: range2478762
display id: range2478762
version: 1
top level: BBa_M45217
direction: direction/forward
start: 1
end: 3
orientation: inline

Stop Codon
type: SequenceAnnotation
persistent identity: annotation2478763
display id: annotation2478763
version: 1
top level: BBa_M45217
location: https://synbiohub.org/public/igem/BBa_M45217/annotation2478763/range2478763/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_M45217/annotation2478763/range2478763/1
type: Range
persistent identity: range2478763
display id: range2478763
version: 1
top level: BBa_M45217
direction: direction/forward
start: 622
end: 624
orientation: inline

BBa_M45220
type: ComponentDefinition
persistent identity: BBa M45220
display id: BBa_M45220
version: 1
was generated by: iGEM to SBOL conversion
description: pre-homologous PpRac-1 section
created: 2016-04-24T11:00:00Z
modified: 2016-04-25T05:27:50Z
mutable provenance: Physcomitrella patens
owned by: james
owned by: myers
mutable description: A homologous, though incomplete, section of the PpRac-1 gene to be used as the 5' boundary of our vector to facilitate homologous recombination in Physcomitrella patens.
discontinued: false
dominant: false
experience: experience/None
group u list: _1855_
m user id: 30477
owner id: 30477
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Justin Marriott
type: DnaRegion
role: partType/Other
role: SO:0000110
sequence: https://synbiohub.org/public/igem/BBa_M45220_sequence/1

BBa_M45221
type: ComponentDefinition
persistent identity: BBa M45221
display id: BBa_M45221
version: 1
was generated by: iGEM to SBOL conversion
description: post-homologous PpRac-1 section
created: 2016-04-24T11:00:00Z
modified: 2016-04-25T05:28:16Z
mutable provenance: Physcomitrella patens
owned by: james
owned by: myers
mutable description: A homologous, though incomplete, section of the PpRac-1 gene to be used as the 3' boundary of our vector to facilitate homologous recombination in Physcomitrella patens.
discontinued: false
dominant: false
experience: experience/None
group u list: _1855_
m user id: 30477
owner id: 30477
owning group id: 9
status: status/Planning
bookmark: false
mutable notes: none
star: false
creator: Justin Marriott
type: DnaRegion
role: SO:0000110
role: partType/Other
sequence: https://synbiohub.org/public/igem/BBa_M45221_sequence/1

BBa_K1159307
type: ComponentDefinition
persistent identity: BBa K1159307
display id: BBa_K1159307
version: 1
was generated by: iGEM to SBOL conversion
description: 35S Terminator of Cauliflower Mosaic Virus (CaMV)
created: 2013-09-13T11:00:00Z
modified: 2015-06-11T01:46:04Z
mutable provenance: Cauliflower Mosaic Virus
owned by: james
owned by: myers
mutable description: This part contains the t35S terminator of the Cauliflower Mosaic Virus (CaMV), a plant virus. This part is flanked by RFC[10] pre- and suffix.
discontinued: false
dominant: false
experience: experience/None
group u list: _1471_
m user id: 4206
owner id: 11507
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: x
star: false
creator: TU Munich 2013
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: CaMV 35S Terminator
sequence: https://synbiohub.org/public/igem/BBa_K1159307_sequence/1

CaMV 35S Terminator
type: SequenceAnnotation
persistent identity: annotation2341614
display id: annotation2341614
version: 1
top level: BBa_K1159307
location: https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_K1159307/annotation2341614/range2341614/1
type: Range
persistent identity: range2341614
display id: range2341614
version: 1
top level: BBa_K1159307
direction: direction/forward
start: 1
end: 217
orientation: inline

https://synbiohub.org/public/igem/BBa_M45222_sequence/1
type: Sequence
persistent identity: BBa M45222 sequence
display id: BBa_M45222_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcggattatgggatacagcaggtaagagacaggattcgactagaatctctcctttgtacctattgtggttactgtcgggatcacgggtgctgattcacattaatttaggacgggcgctgcactacgcattgttgtatgtaatgtttttggtcttgctgattcacacacaaccttccattggaagtcgaccccttagttatttcttattccatttgtcactgaaaacatttactaccctatgcaggtcaagaagattacaacagacttcgacctctgagttacagaggcgctgatgtttttcttctagcattttctcttatcagcaaggctagttatgaaaatatctcaaagaaggtagttatttccttgacgatcttgtcttcgtgttagggttgacgacttgcttgaaaatgtttcaaagtagagaaccgcgtagagggtgctatttacgtcatattttctgatattgcattgcattgcagtgcatccctgagctctactagaggaagatttgttgaagaaaagttacatgcataatgcctcttatacatgccatgtggatatgatttttaaataaaacaatgcttacattttaaaagttgctatagaagtatattacttcaatctttggcccacaagcctcatgaacttcaagaagcttctacatgaacttcttattatatactatatacaaaagtaaaactaaagccatactaaatattgtagccttgattcaaggctctctaatattttttgtaattgacaattgctattgtaatatctttatatattatcacatatttatttatttatttataatactttttctcccttcttagtatgcatgtatactcttctatagattttgtaaatttatgtctttgatatgtaaagttgtaaataatactatattgttttaacaagtattgttaatttttactacaattctaaaattctacatccaaacttaaaagaactaaacttatataaaatttaacatttaataattgtgttcaaatcaatgaataaattatactataatattaaaaaaaaattatacaaaggtatgcatttataatattgtaataaaaaaaatgtaatcaaaattaatatataaaaaatgatatatgtgcagatttcaaattttggaagtaaaagggcgtatttttttgataatccggctctaagtcacaatctttttgctatttgatttaacttgtccttaactttaatgttgattttcaaaactctatacacatattttggcaaagtcaagagtcttcaagtacacaacacattcttttcccctaatacgtcatagtgtcagctaattttgatccactgctttaataaaaactcaaacattattcaaacaaaaaaactcaattcttccatcaaatatattcataaaactgtgataatcctggtttaatttatccaatggaatgccagggtggcgattgaaagagccccacagaaaatcacgatatttcacaggtccaccctactagagaccatcgacagaaccatatccactcgattcatggcagcttttgacgaccatccctagttccgatcacgcacgcacaccggctttgccacgcataccgacttgattgcagtcacagcgcttgccgcgacgccaatccgttcccttgtaccgcatcctgacatttgtccattcgtgtttttgttttttgggttttgagttttgatcgagtactagatggtatcgaagggcgaagaggataacatggctataattaaggagtttatgagattcaaggtccatatggagggcagtgtgaatggacacgagttcgaaattgagggggagggtgaaggtaggccgtacgagggcactcagacagcaaagctcaaggtgaccaaaggcggtccgctaccgttcgcgtgggatatcctgtcaccgcagttcatgtacggttcgaaggcctacgtgaagcatcctgccgacatacctgactacttgaaactgt…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45211_sequence/1
type: Sequence
persistent identity: BBa M45211 sequence
display id: BBa_M45211_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaagatttgttgaagaaaagttacatgcataatgcctcttatacatgccatgtggatatgatttttaaataaaacaatgcttacattttaaaagttgctatagaagtatattacttcaatctttggcccacaagcctcatgaacttcaagaagcttctacatgaacttcttattatatactatatacaaaagtaaaactaaagccatactaaatattgtagccttgattcaaggctctctaatattttttgtaattgacaattgctattgtaatatctttatatattatcacatatttatttatttatttataatactttttctcccttcttagtatgcatgtatactcttctatagattttgtaaatttatgtctttgatatgtaaagttgtaaataatactatattgttttaacaagtattgttaatttttactacaattctaaaattctacatccaaacttaaaagaactaaacttatataaaatttaacatttaataattgtgttcaaatcaatgaataaattatactataatattaaaaaaaaattatacaaaggtatgcatttataatattgtaataaaaaaaatgtaatcaaaattaatatataaaaaatgatatatgtgcagatttcaaattttggaagtaaaagggcgtatttttttgataatccggctctaagtcacaatctttttgctatttgatttaacttgtccttaactttaatgttgattttcaaaactctatacacatattttggcaaagtcaagagtcttcaagtacacaacacattcttttcccctaatacgtcatagtgtcagctaattttgatccactgctttaataaaaactcaaacattattcaaacaaaaaaactcaattcttccatcaaatatattcataaaactgtgataatcctggtttaatttatccaatggaatgccagggtggcgattgaaagagccccacagaaaatcacgatatttcacaggtccaccc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45212_sequence/1
type: Sequence
persistent identity: BBa M45212 sequence
display id: BBa_M45212_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcttttttttctttttttttgtgtttgtttactgttaatttgcaaaaaaacgtttccatgaaaggtttgtaattactgatcacaactcaccgatttaggagagaagcgaacattgcatccacaaattggagtgtttcgatgaggaacttgagagattcatgagatggcgcagcttgggggagttcaggtttagggtttgaggttcaggcctggttgtggagatgttgcgacattaagctcttcaatgtttttgcagggagattcatcaagatatgtgcttctcagggaccttgctccgtcagccaaggcggctgggtagaaatacgccgctcctggtattgtaaacagaaagtagatgcaactctttgcgaattgaatgtttgctatgccacagaccacctcgaaaacactcggaaaaacaagcactgtattatgggacatgtcatcttgcaacttctcatttttctacctcaattttggtctcccaatggttctctaagcatgcattcgtttgtaacttgcaccgatctcaccctccatttcgatggctggagtaatgctcgattgtggggctaatcacgaggaaaagaacgtgttgcaattctccggattccggaactgcttttggccagctctgagggagtggcaccctacaggaatcgagacctagttttatcttaccgtatccgaccatatgaccatcagaccatggacatctggcctgcttccctaaccccacctgttcaacatgccacagagattttcggtaatcacggtcgtattcaattccatctttttaaatttgtgattactctcgtgttgtgaagtgcggattccagttggtcattctttgcagtgcagctttaattgcttaatttccctggttgctttgaggctggatagattgttcgagagtctctcgatttcagttgtctgctgttgtggtttctgctctaatttttatcttcattttttttttttttttttttttttttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45214_sequence/1
type: Sequence
persistent identity: BBa M45214 sequence
display id: BBa_M45214_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: accatcgacagaaccatatccactcgattcatggcagcttttgacgaccatccctagttccgatcacgcacgcacaccggctttgccacgcataccgacttgattgcagtcacagcgcttgccgcgacgccaatccgttcccttgtaccgcatcctgacatttgtccattcgtgtttttgttttttgggttttgagttttgatcgag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45215_sequence/1
type: Sequence
persistent identity: BBa M45215 sequence
display id: BBa_M45215_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggtatcgaagggcgaagaggataacatggctataattaaggagtttatgagattcaaggtccatatggagggcagtgtgaatggacacgagttcgaaattgagggggagggtgaaggtaggccgtacgagggcactcagacagcaaagctcaaggtgaccaaaggcggtccgctaccgttcgcgtgggatatcctgtcaccgcagttcatgtacggttcgaaggcctacgtgaagcatcctgccgacatacctgactacttgaaactgtcttttcccgaggggttcaagtgggaacgtgtcatgaatttcgaagacggaggcgtggtcacggtaactcaggattcctcactacaagatggcgagttcatctataaggtgaagttaagggggactaattttccatctgatgggcccgttatgcaaaaaaagacgatgggctgggaggcctcgtccgagcgtatgtacccagaggacggagccttgaagggtgaaattaaacagcgcttaaaactgaaggacggggggcactatgacgctgaagtaaagacgacttacaaggcaaagaagcctgttcaacttcctggggcctacaatgtgaatatcaagttagatatcacatcgcataacgaggactatacgattgtagagcaatatgaaagagcggaaggcaggcacagtactggaggcatggatgaactttataaatag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45217_sequence/1
type: Sequence
persistent identity: BBa M45217 sequence
display id: BBa_M45217_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgggctgtatcaagtcaaagagaaaggacaatcttaatgatgacgaagctgcgggctcaatggtatcgaaaggggaggagttattcacaggggtcgttccgatcctggtggagctggacggcgatgtcaacggacataaatttagcgtgtctggagaaggagaaggcgatgcgacctatggtaaattgactctaaagtttatttgcaccacgggtaagcttcctgttccttggccaactctggtgactaccttcggatacggcttgatgtgttttgcgcgctacccggatcacatgaagcaacatgacttctttaagagcgctatgcctgagggatatgtccaagaacgaacaattttttttaaagatgatggtaactataaaaccagggctgaggtgaaattcgagggggacacgcttgtgaatcgcatcgagcttaaaggaattgattttaaggaggacggtaatattctgggacataaactggagtacaactataactctcataatgtgtacatcatggctgataagcaaaagaatggaatcaaggttaacttcaagattaggcacaacattgaggacggaagtgtacagctcgcagatcactaccaacaaaacacctag
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45220_sequence/1
type: Sequence
persistent identity: BBa M45220 sequence
display id: BBa_M45220_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atctcggattatgggatacagcaggtaagagacaggattcgactagaatctctcctttgtacctattgtggttactgtcgggatcacgggtgctgattcacattaatttaggacgggcgctgcactacgcattgttgtatgtaatgtttttggtcttgctgattcacacacaaccttccattggaagtcgaccccttagttatttcttattccatttgtcactgaaaacatttactaccctatgcaggtcaagaagattacaacagacttcgacctctgagttacagaggcgctgatgtttttcttctagcattttctcttatcagcaaggctagttatgaaaatatctcaaagaaggtagttatttccttgacgatcttgtcttcgtgttagggttgacgacttgcttgaaaatgtttcaaagtagagaaccgcgtagagggtgctatttacgtcatattttctgatattgcattgcattgcagtgcatccctgagctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45221_sequence/1
type: Sequence
persistent identity: BBa M45221 sequence
display id: BBa_M45221_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: agacactatgcgccatcggtacccatcattctcgttgggacgaagctaggtataaaactccggcttcacttgtggtttgggaatgtgctactgacaagtttattcaatcggatgtaatccacaatgctgaaaatcgaacaaaacattctagcacggatgcgctgtgctcttgcttgtcatttttattctcaagtatttttcactctcttacagctggcgcgtcaattggctgatcttcgagatgataaacagttctttgctgaccatcctggagcggctccaattacaacctctcaagtaagttgaagtatgcatgcattgctcccaaatttaacgtaatgcaagtagtctttcaaagaagcccttgagttatcggaaagttaagatgttgtctttggtagccagtcatgagtactccaattgatggccctcgtacacaattttcagctatggcctagcctgtgctgactggagctctcgtctggatcacgtgttgtcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K1159307_sequence/1
type: Sequence
persistent identity: BBa K1159307 sequence
display id: BBa_K1159307_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctagagtccgcaaaaatcaccagtctctctctacaaatctatctctctctatttttctccagaataatgtgtgagtagttcccagataagggaattagggttcttatagggtttcgctcatgtgttgagcatataagaaacccttagtatgtatttgtatttgtaaaatacttctatcaataaaatttctaattcctaaaaccaaaatccagtgacc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z