##gff-version 3
##sequence-region BBa_M45414 1 731
BBa_M45414	.	ribosome_entry_site	25	39	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M45414	.	ribosome_entry_site	32	36	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M45414	.	engineered_region	25	39	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M45414	.	ribosome_entry_site	25	39	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M45414	.	CDS	46	731	.	+	0	ID=BBa_M45403;Name=BBa_M45403
BBa_M45414	.	stop_codon	728	731	.	+	0	ID=annotation2430609;Name=stop;Parent=BBa_M45403
BBa_M45414	.	start_codon	46	48	.	+	0	ID=annotation2430588;Name=start;Parent=BBa_M45403
BBa_M45414	.	sequence_alteration	157	157	.	+	0	ID=annotation2430613;Name=c to t;Parent=BBa_M45403
BBa_M45414	.	CDS	46	731	.	+	0	ID=annotation2430603;Name=coding;Parent=BBa_M45403
BBa_M45414	.	promoter	1	16	.	+	0	ID=BBa_I760005;Name=BBa_I760005
>BBa_M45414
atgacaaaattgtcattactagagattaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgc
aaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacct
gcaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactg
ctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgctt
cggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcga
gaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaac
gcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctg
ccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcc
tgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaa
cgatgaaggtagtgccctga