##gff-version 3
##sequence-region BBa_M45447 1 462
BBa_M45447	.	sequence_alteration	402	402	.	+	0	ID=annotation2430693;Name=SmaI mutation
BBa_M45447	.	start_codon	1	3	.	+	0	ID=annotation2430691;Name=Start
BBa_M45447	.	sequence_alteration	462	462	.	+	0	ID=annotation2430692;Name=PstI mutation
>BBa_M45447
atgtctttgtcgggcgccggtgccggcccactacccactggttttgggatgcccccacaaagtggttcctcaacgctca
cagagtttaccaaacgtcgaaattggtcgcagcgaatcatcgaggagctgaaagattttctccacatcctaacccctga
cgggcggattctgtatgtgtctccatcttgcaaggctataactggctacgagccacattctctgattggcaagttcatc
ggcgaatacgtccacccagacgatagcggcatttttatgagggagttcaacgagtcaatagcatccggaaacgcgctgc
gctttttctacagatttaagaaggatgacgggacctatatgatctttgagtgtgacggtcatcctcacatatcaccaga
accccctggagctactacctttggccagacccccacaggtcagaccctgcccgtatcaggtctctgtaggggcttcttt
atgatggctcgaccatatcccaccaagaacgctgccctgctcgatagctttttggagcacaaaatcgagaatgagaggt
tgatgcgacgtatcgaagagctgaagagagaagaaatggaagagaacgccgaacaagaaagactgtaccgggagcaggc
ttcatctcaagcgggcgctactccctcacactctggccctgctgggtcactcgcacaggatggcctgagcgaagcccct
aatgctacgccaaattatcaggggatgccacctcctgccaagcctccaatcagtaacacagccctgaccaggcagaacc
tggactcggccctctcggcccagaagccagactccatcgctgacaaaatgagcagatacgaggctagtaacacgcacct
cgagagtattgagctcctgaccggtctccgctatcgggatggcgagcgctctcatggaatttctactggagacacgagt
ccagccctcattcggggtgatgctggtattgccatcttgttggaccgggaccgggaatcgagcaagaaagaaaagaaaa
aattgaaggtagcagacgaatatgtgtgtgcggactgcgggacgctggatagcccagaatggcgcaaaggaccaaaggg
acccaagaccctatgtaacgcctgcgggttgcgctgggctaagaaggagaagaaaagaaccggagacgacgtaaagact
aacagtccagggggtgccatgagcgcccagcctatgcctccagccggtcttacctccgcaggcatgcctccagggatgc
cattacagcactctaccgactcctcaggcggcacacatgggatg