BBa_I746360
1
BBa_I746360
PF promoter from P2 phage
2007-09-10T11:00:00Z
2015-08-31T04:08:04Z
The original source is the P2 phage genome.
For our work the DNA came from plasmids, kindly supplied to us by Prof. Calendar, University of California (Berkeley): Bacteriophage PSP3 and fR73 Activator Proteins: Analysis of Promoter Specificities; Julien and Calendar, 1996; JOURNAL OF BACTERIOLOGY, Oct. 1996, p. 5668???5675
Released HQ 2013
This is the PF promoter taken from the P2 phage genome. It is an inducible promoter that is activated by a class of activators, including P2 ogr (I746350), PSP3 pag (I746351) and phiR73 delta (I746352). These different activators should cause different levels of activity of the PF promoter.
false
false
_116_
0
2122
9
In stock
false
no special considerations seemed necessary
true
Stefan Milde
annotation1943783
1
PF
range1943783
1
1
91
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_M45503
1
BBa_M45503
PF + RBS
2015-04-20T11:00:00Z
2015-05-08T01:14:09Z
bla
wwwwww
false
false
_1855_
0
25693
9
Not in stock
false
ha
false
Angela Akude
component2431465
1
BBa_B0034
component2431463
1
BBa_I746360
annotation2431465
1
BBa_B0034
range2431465
1
100
111
annotation2431463
1
BBa_I746360
range2431463
1
1
91
BBa_B0034_sequence
1
aaagaggagaaa
BBa_M45503_sequence
1
ttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagaggagaaa
BBa_I746360_sequence
1
ttgcccgccttttctttaccggtggttgtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccact
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z