LOCUS       BBa_M45504              1904 bp    DNA     linear       22-Nov-2024
DEFINITION  PF promoter with cell lysis device
ACCESSION   BBa_M45504
VERSION     BBa_M45504.1  
FEATURES             Location/Qualifiers
     promoter        1..91
                     /label=PF
     promoter        1..91
                     /label=BBa_I746360
     misc_feature    104..107
                     /label=conserved
     RBS             100..111
                     /label=BBa_B0034
     misc_feature    1..111
                     /label=BBa_M45503
     CDS             136..792
                     /label=T4 holin
     CDS             120..795
                     /label=BBa_K112805
     CDS             820..1314
                     /label=T4 Endolysin
     CDS             804..1317
                     /label=BBa_K112806
     stem_loop       1337..1380
                     /label=stem_loop
     misc_feature    1326..1405
                     /label=BBa_B0010
     terminator      1326..1405
                     /label=BBa_B0010
     stem_loop       1421..1440
                     /label=T7 TE
     misc_feature    1447..1447
                     /label=stop
     polyA_site      1441..1454
                     /label=polya
     misc_feature    1414..1454
                     /label=BBa_B0012
     terminator      1414..1454
                     /label=BBa_B0012
     promoter        1463..1497
                     /label=BBa_J23116
     CDS             1520..1813
                     /label=T4 antiholin
     CDS             1506..1816
                     /label=BBa_K112807
     stem_loop       1836..1879
                     /label=stem_loop
     misc_feature    1825..1904
                     /label=BBa_B0010
     terminator      1825..1904
                     /label=BBa_B0010
     misc_feature    120..1904
                     /label=BBa_K112808
ORIGIN
        1 ttgcccgcct tttctttacc ggtggttgtg ctgtcgatta gccaaccggg acaaatagcc
       61 tgacatctcc ggcgcaactg aaaataccac ttactagaga aagaggagaa atactagagc
      121 ttaaaaggag ggtctatggc agcacctaga atatcatttt cgccctctga tattctattt
      181 ggtgttctcg atcgcttgtt caaagataac gctaccggga aggttcttgc ttcccgggta
      241 gctgtcgtaa ttcttttgtt tataatggcg attgtttggt ataggggaga tagtttcttt
      301 gagtactata agcaatcaaa gtatgaaaca tacagtgaaa ttattgaaaa ggaaagaact
      361 gcacgctttg aatctgtcgc cctggaacaa ctccagatag ttcatatatc atctgaggca
      421 gactttagtg cggtgtattc tttccgccct aaaaacttaa actattttgt tgatattata
      481 gcatacgaag gaaaattacc ttcaacaata agtgaaaaat cacttggagg atatcctgtt
      541 gataaaacta tggatgaata tacagttcat ttaaatggac gtcattatta ttccaactca
      601 aaatttgctt ttttaccaac taaaaagcct actcccgaaa taaactacat gtacagttgt
      661 ccatatttta atttggataa tatctatgct ggaacgataa ccatgtactg gtatagaaat
      721 gatcatataa gtaatgaccg ccttgaatca atatgtgctc aggcggccag aatattagga
      781 agggctaaat aattatacta gagatactta ggaggtatta tgaatatatt tgaaatgtta
      841 cgtatagatg aaggtcttag acttaaaatc tataaagaca cagaaggcta ttacactatt
      901 ggcatcggtc atttgcttac aaaaagtcca tcacttaatg ctgctaaatc tgaattagat
      961 aaagctattg ggcgtaattg caatggtgta attacaaaag atgaggctga aaaactcttt
     1021 aatcaggatg ttgatgctgc tgttcgcgga atcctgagaa atgctaaatt aaaaccggtt
     1081 tatgattctc ttgatgcggt tcgtcgctgt gcattgatta atatggtttt ccaaatggga
     1141 gaaaccggtg tggcaggatt tactaactct ttacgtatgc ttcaacaaaa acgctgggat
     1201 gaagcagcag ttaacttagc taaaagtaga tggtataatc aaacacctaa tcgcgcaaaa
     1261 cgagtcatta caacgtttag aactggcact tgggacgcgt ataaaaatct ataaagctac
     1321 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
     1381 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
     1441 tttctgcgtt tatatactag agttgacagc tagctcagtc ctagggacta tgctagctac
     1501 tagaggatag gaggccttta tggccttaaa agcaacagca ctttttgcca tgctaggatt
     1561 gtcatttgtt ttatctccat cgattgaagc gaatgtcgat cctcattttg ataaatttat
     1621 ggaatctggt attaggcacg tttatatgct ttttgaaaat aaaagcgtag aatcgtctga
     1681 acaattctat agttttatga gaacgaccta taaaaatgac ccgtgctctt ctgattttga
     1741 atgtatagag cgaggcgcgg agatggcaca atcatacgct agaattatga acattaaatt
     1801 ggagactgaa tgaaattact agagccaggc atcaaataaa acgaaaggct cagtcgaaag
     1861 actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctc
//