LOCUS       BBa_M45505               491 bp    DNA     linear       26-Dec-2024
DEFINITION  pRM promoter with phiR73 delta that is used to activate the 
            promoter PF
ACCESSION   BBa_M45505
VERSION     BBa_M45505.1  
FEATURES             Location/Qualifiers
     misc_feature    9..25
                     /label=OR1
     misc_feature    33..49
                     /label=OR2
     promoter        48..53
                     /label=-35
     misc_feature    59..65
                     /label=mutate to obliterate OR3
     promoter        71..76
                     /label=-10
     promoter        1..82
                     /label=BBa_I12007
     misc_feature    91..102
                     /label=B0034
     CDS             112..354
                     /label=phiR73 delta
     misc_feature    112..112
                     /label=phiR73 delta
     misc_feature    192..192
                     /label=silent mutation to remove PstI site
     misc_feature    91..354
                     /label=BBa_I746352
     stem_loop       374..417
                     /label=stem_loop
     misc_feature    363..442
                     /label=BBa_B0010
     terminator      363..442
                     /label=BBa_B0010
     stem_loop       458..477
                     /label=T7 TE
     misc_feature    484..484
                     /label=stop
     polyA_site      478..491
                     /label=polya
     misc_feature    451..491
                     /label=BBa_B0012
     terminator      451..491
                     /label=BBa_B0012
     terminator      363..491
                     /label=BBa_B0015
ORIGIN
        1 gcaaccatta tcaccgccag aggtaaaata gtcaacacgc acggtgttag atatttataa
       61 atagtggtga tagatttaac gttactagag aaagaggaga aatactagat gatgcgctgc
      121 cctttctgtc gtcattcagc gcatacccgc accagccggt atgtgagtga caatgtcaaa
      181 gaaagttatc tccagtgcca gaatatttac tgttcggcga catttaaaac gcatgagtca
      241 atttgtgccg tgattcgttc tccggtcacg gaggaaaaac cagcaccggc aagcacagca
      301 ccggctgttg tccgaaaagt taaaggctgt tacagctcac cattcaacca ttaatactag
      361 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      421 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
      481 ctgcgtttat a
//