LOCUS       BBa_M45506               974 bp    DNA     linear       23-Dec-2024
DEFINITION  PF + RBS + GFP + B0015
ACCESSION   BBa_M45506
VERSION     BBa_M45506.1  
FEATURES             Location/Qualifiers
     promoter        1..91
                     /label=PF
     promoter        1..91
                     /label=BBa_I746360
     misc_feature    104..107
                     /label=conserved
     RBS             100..111
                     /label=BBa_B0034
     misc_feature    1..111
                     /label=BBa_M45503
     CDS             118..837
                     /label=GFP protein
     CDS             118..837
                     /label=BBa_E0040
     stem_loop       857..900
                     /label=stem_loop
     misc_feature    846..925
                     /label=BBa_B0010
     terminator      846..925
                     /label=BBa_B0010
     stem_loop       941..960
                     /label=T7 TE
     misc_feature    967..967
                     /label=stop
     polyA_site      961..974
                     /label=polya
     misc_feature    934..974
                     /label=BBa_B0012
     terminator      934..974
                     /label=BBa_B0012
     terminator      846..974
                     /label=BBa_B0015
ORIGIN
        1 ttgcccgcct tttctttacc ggtggttgtg ctgtcgatta gccaaccggg acaaatagcc
       61 tgacatctcc ggcgcaactg aaaataccac ttactagaga aagaggagaa atactagatg
      121 cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt agatggtgat
      181 gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac atacggaaaa
      241 cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc aacacttgtc
      301 actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat gaaacagcat
      361 gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat atttttcaaa
      421 gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac ccttgttaat
      481 agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg acacaaattg
      541 gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa gaatggaatc
      601 aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact agcagaccat
      661 tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa ccattacctg
      721 tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat ggtccttctt
      781 gagtttgtaa cagctgctgg gattacacat ggcatggatg aactatacaa ataataatac
      841 tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat
      901 ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt cgggtgggcc
      961 tttctgcgtt tata
//