LOCUS       BBa_M45507              1963 bp    DNA     linear       23-Dec-2024
DEFINITION  pRM + RBS + GFP + Stop Codon + RBS + phiR73 delta + GFP + B0015
ACCESSION   BBa_M45507
VERSION     BBa_M45507.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    29..45
                     /label=OR1
     misc_feature    53..69
                     /label=OR2
     promoter        68..73
                     /label=-35
     misc_feature    79..85
                     /label=mutate to obliterate OR3
     promoter        91..96
                     /label=-10
     promoter        21..102
                     /label=BBa_I12007
     misc_feature    1..102
                     /label=BBa_M45500
     CDS             109..828
                     /label=GFP protein
     CDS             109..828
                     /label=BBa_E0040
     misc_feature    1..828
                     /label=BBa_M45511
     misc_feature    837..848
                     /label=B0034
     CDS             858..1100
                     /label=phiR73 delta
     misc_feature    858..858
                     /label=phiR73 delta
     misc_feature    938..938
                     /label=silent mutation to remove PstI site
     misc_feature    837..1100
                     /label=BBa_I746352
     CDS             1107..1826
                     /label=GFP protein
     CDS             1107..1826
                     /label=BBa_E0040
     stem_loop       1846..1889
                     /label=stem_loop
     misc_feature    1835..1914
                     /label=BBa_B0010
     terminator      1835..1914
                     /label=BBa_B0010
     stem_loop       1930..1949
                     /label=T7 TE
     misc_feature    1956..1956
                     /label=stop
     polyA_site      1950..1963
                     /label=polya
     misc_feature    1923..1963
                     /label=BBa_B0012
     terminator      1923..1963
                     /label=BBa_B0012
     terminator      1835..1963
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagag gcaaccatta tcaccgccag aggtaaaata gtcaacacgc
       61 acggtgttag atatttataa atagtggtga tagatttaac gttactagat gcgtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      781 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagaaag
      841 aggagaaata ctagatgatg cgctgccctt tctgtcgtca ttcagcgcat acccgcacca
      901 gccggtatgt gagtgacaat gtcaaagaaa gttatctcca gtgccagaat atttactgtt
      961 cggcgacatt taaaacgcat gagtcaattt gtgccgtgat tcgttctccg gtcacggagg
     1021 aaaaaccagc accggcaagc acagcaccgg ctgttgtccg aaaagttaaa ggctgttaca
     1081 gctcaccatt caaccattaa tactagatgc gtaaaggaga agaacttttc actggagttg
     1141 tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct gtcagtggag
     1201 agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc actactggaa
     1261 aactacctgt tccatggcca acacttgtca ctactttcgg ttatggtgtt caatgctttg
     1321 cgagataccc agatcatatg aaacagcatg actttttcaa gagtgccatg cccgaaggtt
     1381 atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca cgtgctgaag
     1441 tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt gattttaaag
     1501 aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac aatgtataca
     1561 tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga cacaacattg
     1621 aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt ggcgatggcc
     1681 ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg aaagatccca
     1741 acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg attacacatg
     1801 gcatggatga actatacaaa taataatact agagccaggc atcaaataaa acgaaaggct
     1861 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1921 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//