BBa_M45507
type: ComponentDefinition
persistent identity: BBa M45507
display id: BBa_M45507
version: 1
was generated by: iGEM to SBOL conversion
description: pRM + RBS + GFP + Stop Codon + RBS + phiR73 delta + GFP + B0015
created: 2015-04-22T11:00:00Z
modified: 2015-05-08T01:14:09Z
mutable provenance: This composite part was obtained from basic parts in the registry.
owned by: james
owned by: myers
mutable description: Testing how well the signal passes through kill switch.
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 25693
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Review scars
star: false
creator: Angela Akude
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_M45511
component: BBa_E0040
component: BBa_I746352
component: BBa_B0015
sequence annotation: BBa_M45511
sequence annotation: BBa_I746352
sequence annotation: BBa_E0040
sequence annotation: BBa_B0015
sequence: https://synbiohub.org/public/igem/BBa_M45507_sequence/1

BBa_M45511
type: Component
persistent identity: component2431657
display id: component2431657
version: 1
top level: BBa_M45507
definition: BBa_M45511
access: public

BBa_E0040
type: Component
persistent identity: component2431664
display id: component2431664
version: 1
top level: BBa_M45507
definition: GFP
access: public

BBa_I746352
type: Component
persistent identity: component2431662
display id: component2431662
version: 1
top level: BBa_M45507
definition: BBa_I746352
access: public

BBa_B0015
type: Component
persistent identity: component2431671
display id: component2431671
version: 1
top level: BBa_M45507
definition: BBa_B0015
access: public

BBa_M45511
type: SequenceAnnotation
persistent identity: annotation2431657
display id: annotation2431657
version: 1
top level: BBa_M45507
location: https://synbiohub.org/public/igem/BBa_M45507/annotation2431657/range2431657/1
component: BBa_M45511

https://synbiohub.org/public/igem/BBa_M45507/annotation2431657/range2431657/1
type: Range
persistent identity: range2431657
display id: range2431657
version: 1
top level: BBa_M45507
direction: direction/reverse
start: 1
end: 828
orientation: inline

BBa_I746352
type: SequenceAnnotation
persistent identity: annotation2431662
display id: annotation2431662
version: 1
top level: BBa_M45507
location: https://synbiohub.org/public/igem/BBa_M45507/annotation2431662/range2431662/1
component: BBa_I746352

https://synbiohub.org/public/igem/BBa_M45507/annotation2431662/range2431662/1
type: Range
persistent identity: range2431662
display id: range2431662
version: 1
top level: BBa_M45507
direction: direction/reverse
start: 837
end: 1100
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2431664
display id: annotation2431664
version: 1
top level: BBa_M45507
location: https://synbiohub.org/public/igem/BBa_M45507/annotation2431664/range2431664/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_M45507/annotation2431664/range2431664/1
type: Range
persistent identity: range2431664
display id: range2431664
version: 1
top level: BBa_M45507
direction: direction/reverse
start: 1107
end: 1826
orientation: inline

BBa_B0015
type: SequenceAnnotation
persistent identity: annotation2431671
display id: annotation2431671
version: 1
top level: BBa_M45507
location: https://synbiohub.org/public/igem/BBa_M45507/annotation2431671/range2431671/1
component: BBa_B0015

https://synbiohub.org/public/igem/BBa_M45507/annotation2431671/range2431671/1
type: Range
persistent identity: range2431671
display id: range2431671
version: 1
top level: BBa_M45507
direction: direction/reverse
start: 1835
end: 1963
orientation: inline

GFP
type: ComponentDefinition
persistent identity: BBa E0040
display id: BBa_E0040
version: 1
was generated by: iGEM to SBOL conversion
description: green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212
created: 2004-09-29T11:00:00Z
modified: 2016-01-26T02:09:38Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: GFP (mut3b) [note that this part does not have a barcode]
discontinued: false
dominant: true
experience: experience/Works
group u list: _11_1_
m user id: 4206
owner id: 61
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: jcbraff
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: GFP protein
sequence: https://synbiohub.org/public/igem/BBa_E0040_sequence/1

GFP protein
type: SequenceAnnotation
persistent identity: annotation1934520
display id: annotation1934520
version: 1
top level: GFP
location: https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
role: feature/protein
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0040/annotation1934520/range1934520/1
type: Range
persistent identity: range1934520
display id: range1934520
version: 1
top level: GFP
direction: direction/forward
start: 1
end: 720
orientation: inline

BBa_B0015
type: ComponentDefinition
persistent identity: BBa B0015
display id: BBa_B0015
version: 1
was generated by: iGEM to SBOL conversion
description: double terminator (B0010-B0012)
created: 2003-07-16T11:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Double terminator consisting of BBa_B0010 and BBa_B0012
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Reshma Shetty
type: DnaRegion
role: partType/Terminator
role: SO:0000141
component: BBa_B0012
component: BBa_B0010
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence: https://synbiohub.org/public/igem/BBa_B0015_sequence/1

BBa_B0012
type: Component
persistent identity: component1916612
display id: component1916612
version: 1
top level: BBa_B0015
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1916610
display id: component1916610
version: 1
top level: BBa_B0015
definition: BBa_B0010
access: public

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1916610
display id: annotation1916610
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_B0015/annotation1916610/range1916610/1
type: Range
persistent identity: range1916610
display id: range1916610
version: 1
top level: BBa_B0015
direction: direction/forward
start: 1
end: 80
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1916612
display id: annotation1916612
version: 1
top level: BBa_B0015
location: https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_B0015/annotation1916612/range1916612/1
type: Range
persistent identity: range1916612
display id: range1916612
version: 1
top level: BBa_B0015
direction: direction/forward
start: 89
end: 129
orientation: inline

BBa_M45511
type: ComponentDefinition
persistent identity: BBa M45511
display id: BBa_M45511
version: 1
was generated by: iGEM to SBOL conversion
description: Parts M4500, E0040 and Stop Codon
created: 2015-04-20T11:00:00Z
modified: 2015-05-08T01:14:09Z
mutable provenance: Parts registry for all of the parts is the origin of these parts.
owned by: james
owned by: myers
mutable description: This is a part that includes a viral sensing promoter with a ribosomal binding site along with green fluorescent protein. This construct allows for visualizing whether the promoter construct is practical.
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 25691
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The scar site is to be considered and also
star: false
creator: Nathan Hebert
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_M45500
component: BBa_E0040
sequence annotation: BBa_M45500
sequence annotation: BBa_E0040
sequence: https://synbiohub.org/public/igem/BBa_M45511_sequence/1

BBa_M45500
type: Component
persistent identity: component2431395
display id: component2431395
version: 1
top level: BBa_M45511
definition: BBa_M45500
access: public

BBa_E0040
type: Component
persistent identity: component2431397
display id: component2431397
version: 1
top level: BBa_M45511
definition: GFP
access: public

BBa_M45500
type: SequenceAnnotation
persistent identity: annotation2431395
display id: annotation2431395
version: 1
top level: BBa_M45511
location: https://synbiohub.org/public/igem/BBa_M45511/annotation2431395/range2431395/1
component: BBa_M45500

https://synbiohub.org/public/igem/BBa_M45511/annotation2431395/range2431395/1
type: Range
persistent identity: range2431395
display id: range2431395
version: 1
top level: BBa_M45511
direction: direction/reverse
start: 1
end: 102
orientation: inline

BBa_E0040
type: SequenceAnnotation
persistent identity: annotation2431397
display id: annotation2431397
version: 1
top level: BBa_M45511
location: https://synbiohub.org/public/igem/BBa_M45511/annotation2431397/range2431397/1
component: BBa_E0040

https://synbiohub.org/public/igem/BBa_M45511/annotation2431397/range2431397/1
type: Range
persistent identity: range2431397
display id: range2431397
version: 1
top level: BBa_M45511
direction: direction/reverse
start: 109
end: 828
orientation: inline

BBa_I746352
type: ComponentDefinition
persistent identity: BBa I746352
display id: BBa_I746352
version: 1
was generated by: iGEM to SBOL conversion
description: delta activator from phiR73 phage
created: 2007-09-11T11:00:00Z
modified: 2015-08-31T04:08:04Z
mutable provenance: plasmid DNA supplied by Prof. Richard Calendar, University of California.
owned by: james
owned by: myers
mutable description: The delta activator taken from phiR73 phage acts on a class of inducible promoters (parts I746360 to I746365), inducing their activity to varying degrees. The part sequence does already contain a ribosome binding site (B0034)!
discontinued: false
dominant: false
experience: experience/None
group u list: _116_
m user id: 0
owner id: 2122
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The part does contain a RBS (B0034) already.
star: true
creator: Stefan Milde
type: DnaRegion
role: SO:0000804
role: partType/Translational Unit
sequence annotation: B0034
sequence annotation: phiR73 delta
sequence annotation: silent mutation to remove PstI site
sequence: https://synbiohub.org/public/igem/BBa_I746352_sequence/1

B0034
type: SequenceAnnotation
persistent identity: annotation1943871
display id: annotation1943871
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_I746352/annotation1943871/range1943871/1
type: Range
persistent identity: range1943871
display id: range1943871
version: 1
top level: BBa_I746352
direction: direction/forward
start: 1
end: 12
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943872
display id: annotation1943872
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_I746352/annotation1943872/range1943872/1
type: Range
persistent identity: range1943872
display id: range1943872
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 264
orientation: inline

phiR73 delta
type: SequenceAnnotation
persistent identity: annotation1943873
display id: annotation1943873
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
role: feature/start
role: SO:0000318

https://synbiohub.org/public/igem/BBa_I746352/annotation1943873/range1943873/1
type: Range
persistent identity: range1943873
display id: range1943873
version: 1
top level: BBa_I746352
direction: direction/forward
start: 22
end: 22
orientation: inline

silent mutation to remove PstI site
type: SequenceAnnotation
persistent identity: annotation1943874
display id: annotation1943874
version: 1
top level: BBa_I746352
location: https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I746352/annotation1943874/range1943874/1
type: Range
persistent identity: range1943874
display id: range1943874
version: 1
top level: BBa_I746352
direction: direction/forward
start: 102
end: 102
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: SO:0000319
role: feature/stop

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

BBa_M45500
type: ComponentDefinition
persistent identity: BBa M45500
display id: BBa_M45500
version: 1
was generated by: iGEM to SBOL conversion
description: pRM + RBS
created: 2015-04-20T11:00:00Z
modified: 2015-05-08T01:14:09Z
mutable provenance: This a composite part from two parts within the registry, which are I12007 and B0034.
owned by: james
owned by: myers
mutable description: The first basic part of this composite part is a Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI). This allows this promoter to be turned on by the presence of viral produced Cl. The other basic part of this composite part is RBS based on Elowitz repressilator.
discontinued: false
dominant: false
group u list: _1855_
m user id: 0
owner id: 25691
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The scar had to be reviewed in order so no illegal enzymes were formed.
star: false
creator: Nathan Hebert
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0034
component: BBa_I12007
sequence annotation: BBa_B0034
sequence annotation: BBa_I12007
sequence: https://synbiohub.org/public/igem/BBa_M45500_sequence/1

BBa_B0034
type: Component
persistent identity: component2431358
display id: component2431358
version: 1
top level: BBa_M45500
definition: BBa_B0034
access: public

BBa_I12007
type: Component
persistent identity: component2431364
display id: component2431364
version: 1
top level: BBa_M45500
definition: Prm +
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2431358
display id: annotation2431358
version: 1
top level: BBa_M45500
location: https://synbiohub.org/public/igem/BBa_M45500/annotation2431358/range2431358/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_M45500/annotation2431358/range2431358/1
type: Range
persistent identity: range2431358
display id: range2431358
version: 1
top level: BBa_M45500
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_I12007
type: SequenceAnnotation
persistent identity: annotation2431364
display id: annotation2431364
version: 1
top level: BBa_M45500
location: https://synbiohub.org/public/igem/BBa_M45500/annotation2431364/range2431364/1
component: BBa_I12007

https://synbiohub.org/public/igem/BBa_M45500/annotation2431364/range2431364/1
type: Range
persistent identity: range2431364
display id: range2431364
version: 1
top level: BBa_M45500
direction: direction/reverse
start: 21
end: 102
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

Prm +
type: ComponentDefinition
persistent identity: BBa I12007
display id: BBa_I12007
version: 1
was generated by: iGEM to SBOL conversion
description: Modified lambda Prm promoter (OR-3 obliterated)
created: 2004-07-14T11:00:00Z
modified: 2015-08-31T04:07:31Z
mutable provenance: Shih and Gussin (1983)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Lambda Prm promoter modified to be activated but not repressed by the lambda repressor (cI)
discontinued: false
dominant: true
experience: experience/None
group u list: _3_
m user id: 0
owner id: 103
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: In wild type lambda phage, the OR3 site (-27 to -11) reads "tatcccttgcggtgata" on the sense strand of DNA. To prevent lambda repressor (cI) from binding to this site, the 4th through 10th nt of OR3 were replaced by the 7 nt between OR2 and OR1, "aaatagt" (-57 to -51), which in effect mutates 5 nt of the promoter. These nt were chosen to be about halfway between the -10 and -35 boxes of the promoter. Furthermore, since these nt already act as a spacer in wild-type phage, it is hoped they will not create undesired interactions.
star: true
creator: Hans
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: OR1
sequence annotation: OR2
sequence annotation: -35
sequence annotation: mutate to obliterate OR3
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_I12007_sequence/1

OR1
type: SequenceAnnotation
persistent identity: annotation937701
display id: annotation937701
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
role: feature/binding
role: SO:0001091

https://synbiohub.org/public/igem/BBa_I12007/annotation937701/range937701/1
type: Range
persistent identity: range937701
display id: range937701
version: 1
top level: Prm +
direction: direction/forward
start: 9
end: 25
orientation: inline

OR2
type: SequenceAnnotation
persistent identity: annotation937702
display id: annotation937702
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_I12007/annotation937702/range937702/1
type: Range
persistent identity: range937702
display id: range937702
version: 1
top level: Prm +
direction: direction/forward
start: 33
end: 49
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation937703
display id: annotation937703
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_I12007/annotation937703/range937703/1
type: Range
persistent identity: range937703
display id: range937703
version: 1
top level: Prm +
direction: direction/forward
start: 48
end: 53
orientation: inline

mutate to obliterate OR3
type: SequenceAnnotation
persistent identity: annotation937704
display id: annotation937704
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_I12007/annotation937704/range937704/1
type: Range
persistent identity: range937704
display id: range937704
version: 1
top level: Prm +
direction: direction/forward
start: 59
end: 65
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation937705
display id: annotation937705
version: 1
top level: Prm +
location: https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_I12007/annotation937705/range937705/1
type: Range
persistent identity: range937705
display id: range937705
version: 1
top level: Prm +
direction: direction/forward
start: 71
end: 76
orientation: inline

https://synbiohub.org/public/igem/BBa_M45507_sequence/1
type: Sequence
persistent identity: BBa M45507 sequence
display id: BBa_M45507_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagaaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0040_sequence/1
type: Sequence
persistent identity: BBa E0040 sequence
display id: BBa_E0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0015_sequence/1
type: Sequence
persistent identity: BBa B0015 sequence
display id: BBa_B0015_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45511_sequence/1
type: Sequence
persistent identity: BBa M45511 sequence
display id: BBa_M45511_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgttactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I746352_sequence/1
type: Sequence
persistent identity: BBa I746352 sequence
display id: BBa_I746352_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgatgcgctgccctttctgtcgtcattcagcgcatacccgcaccagccggtatgtgagtgacaatgtcaaagaaagttatctccagtgccagaatatttactgttcggcgacatttaaaacgcatgagtcaatttgtgccgtgattcgttctccggtcacggaggaaaaaccagcaccggcaagcacagcaccggctgttgtccgaaaagttaaaggctgttacagctcaccattcaaccattaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_M45500_sequence/1
type: Sequence
persistent identity: BBa M45500 sequence
display id: BBa_M45500_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagaggcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I12007_sequence/1
type: Sequence
persistent identity: BBa I12007 sequence
display id: BBa_I12007_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gcaaccattatcaccgccagaggtaaaatagtcaacacgcacggtgttagatatttataaatagtggtgatagatttaacgt
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z