LOCUS       BBa_M45512              1237 bp    DNA     linear       23-Dec-2024
DEFINITION  pRM + RBS + GFP + Stop Codon + RBS + phiR 73 delta + B0015
ACCESSION   BBa_M45512
VERSION     BBa_M45512.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    29..45
                     /label=OR1
     misc_feature    53..69
                     /label=OR2
     promoter        68..73
                     /label=-35
     misc_feature    79..85
                     /label=mutate to obliterate OR3
     promoter        91..96
                     /label=-10
     promoter        21..102
                     /label=BBa_I12007
     misc_feature    1..102
                     /label=BBa_M45500
     CDS             109..828
                     /label=GFP protein
     CDS             109..828
                     /label=BBa_E0040
     misc_feature    1..828
                     /label=BBa_M45511
     misc_feature    837..848
                     /label=B0034
     CDS             858..1100
                     /label=phiR73 delta
     misc_feature    858..858
                     /label=phiR73 delta
     misc_feature    938..938
                     /label=silent mutation to remove PstI site
     misc_feature    837..1100
                     /label=BBa_I746352
     stem_loop       1120..1163
                     /label=stem_loop
     misc_feature    1109..1188
                     /label=BBa_B0010
     terminator      1109..1188
                     /label=BBa_B0010
     stem_loop       1204..1223
                     /label=T7 TE
     misc_feature    1230..1230
                     /label=stop
     polyA_site      1224..1237
                     /label=polya
     misc_feature    1197..1237
                     /label=BBa_B0012
     terminator      1197..1237
                     /label=BBa_B0012
     terminator      1109..1237
                     /label=BBa_B0015
ORIGIN
        1 aaagaggaga aatactagag gcaaccatta tcaccgccag aggtaaaata gtcaacacgc
       61 acggtgttag atatttataa atagtggtga tagatttaac gttactagat gcgtaaagga
      121 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
      181 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
      241 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
      301 ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca tgactttttc
      361 aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
      421 aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
      481 ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
      541 tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
      601 ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
      661 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
      721 tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
      781 acagctgctg ggattacaca tggcatggat gaactataca aataataata ctagagaaag
      841 aggagaaata ctagatgatg cgctgccctt tctgtcgtca ttcagcgcat acccgcacca
      901 gccggtatgt gagtgacaat gtcaaagaaa gttatctcca gtgccagaat atttactgtt
      961 cggcgacatt taaaacgcat gagtcaattt gtgccgtgat tcgttctccg gtcacggagg
     1021 aaaaaccagc accggcaagc acagcaccgg ctgttgtccg aaaagttaaa ggctgttaca
     1081 gctcaccatt caaccattaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     1141 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     1201 actggctcac cttcgggtgg gcctttctgc gtttata
//