BBa_M45750
1
BBa_M45750
P-rbs-10xhis-EPS-Hd1a-Stop
2015-04-21T11:00:00Z
2015-05-08T01:14:09Z
It is peptide μ-TRTX-Hd1a from the venom of Haplopelma doriae.
Codes for the Hd1a protein which blocks sodium channels, specifically Nav1.1 and Nav1.7, and can be used as an analgesic. A 10x histidine has been included for purification, and an enterokinase protease site has been included to cleave off the histidine tag after purification.
false
false
_1855_
0
25686
9
Not in stock
false
This part has been optimized for use in E. coli.
false
Alex Torgesen
component2431542
1
BBa_K1162009
component2431540
1
BBa_K208010
component2431544
1
BBa_M45751
component2431553
1
BBa_B0015
component2431546
1
BBa_M45711
annotation2431544
1
BBa_M45751
range2431544
1
268
282
annotation2431542
1
BBa_K1162009
range2431542
1
227
259
annotation2431540
1
BBa_K208010
range2431540
1
1
220
annotation2431553
1
BBa_B0015
range2431553
1
404
532
annotation2431546
1
BBa_M45711
range2431546
1
291
395
BBa_M45711
1
BBa_M45711
Hd1a toxin from Haplopelma doriae spider
2015-04-18T11:00:00Z
2015-05-08T01:14:09Z
This sequence comes from the Haplopelma doriae spider.
http://onlinelibrary.wiley.com/doi/10.1111/bph.13081/full
Hd1a shows signs of being possibly used as another analgesic. It effectively blocks the Nav1.7 sodium channel and thus is an effective pain blocker.
false
false
_1855_
0
23855
9
Not in stock
false
This sequence has been codon optimized for RC 10.
false
Nicholas D. Lauritzen
annotation2431527
1
Hd1a
range2431527
1
1
105
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916610
1
BBa_B0010
component1916612
1
BBa_B0012
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
BBa_M45751
1
BBa_M45751
Enterokinase protease site
2015-04-21T11:00:00Z
2015-05-08T01:14:09Z
It comes from the intestines where enterokinase recognizes the site and cuts trypsinogen into its active form of trypsin.
This is the recognition site for enterokinase which cleaves after the lysine leaving no residual amino acids.
false
false
_1855_
0
25686
9
Not in stock
false
Optimized for use in E. coli.
false
Alex Torgesen
annotation2431526
1
enterokinase recognition site
range2431526
1
1
15
BBa_K208010
1
BBa_K208010
Lac Promoter (BBa_R0010) and RBS (B0034)
2009-10-11T11:00:00Z
2015-05-08T01:11:24Z
synthetic
Released HQ 2013
lac + rbs
false
false
_310_
0
3473
9
In stock
true
none
false
Elisabeth Linton
annotation2034625
1
BBa_B0034
range2034625
1
209
220
annotation2034626
1
BBa_R0010
range2034626
1
1
200
BBa_K1162009
1
BBa_K1162009
10x-Histidine (10x-His) Tag with Met (ATG) For N-terminal purification
2013-09-14T11:00:00Z
2015-05-08T01:09:32Z
E. coli
This part uses assembly standard 23 (RFC 23-silver fusion) to allow for protein fusions. This 10x His Tag is similar to BBa_K844000 but has the double stop codon (TAA TAA) removed unlike BBa_K844000. (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000).
Another characteristic of this part is the presence of a Met (ATG) for direct His Tag and protein expression. Since the presence of the Met (ATG) this His Tag should be used for protein purification at the N-terminal. The user can place a promoter-rbs system of their choice in front of this His Tag for direct expression. The lack of any stop codon in this His Tag allows the user to add additional features downstream of the gene e.g. secretion tag or GFP (note, the gene itself must not contain any stop codons if the His Tag is to be used like this).
Related parts
BBa_K844000 (http://parts.igem.org/wiki/index.php?title=Part:BBa_K844000), this link also provides an extensive review of existing Histidine Tags in the Parts Registry.
false
false
_1474_
0
9404
9
It's complicated
true
RFC 23 and contains an atg (met). No stop codon.
false
Kathleen Miller
annotation2343739
1
start
range2343739
1
1
3
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_M45711_sequence
1
gcctgcctcggtttcggtaaatcatgtaacccgagtaatgaccaatgctgcaaatcatcttcattagcttgttcaacaaaacataagtggtgcaaatacgaactg
BBa_K1162009_sequence
1
atgcatcatcaccatcaccaccatcatcaccat
BBa_M45750_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgcatcatcaccatcaccaccatcatcaccattactagaggatgatgatgataaatactagaggcctgcctcggtttcggtaaatcatgtaacccgagtaatgaccaatgctgcaaatcatcttcattagcttgttcaacaaaacataagtggtgcaaatacgaactgtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K208010_sequence
1
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_M45751_sequence
1
gatgatgatgataaa
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z