BBa_M45801 1 BBa_M45801 Bi-directional lactococcal bacteriophage Tuc2009 promoter 2015-04-14T11:00:00Z 2015-09-17T09:37:15Z Kenny JG, Leach S, Hoz AB, Venema G, Kok J, Fitzgerald GF, Nauta S, Alonso JC, Sinderen D (2006) Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009. Virology 347:434-446 Bi-directional promoter containing a divergent rightward (PR) and leftward (PL) promoter from Tuc2009 bacteriophage with an intergenic switch region negatively regulated by CI and Cro repressor proteins, respectively. The intergenic switch region contains four operator regions designated OL-Cro, OR-Cro, OL-CI, and OR-CI. Cro has high affinity binding to OL-Cro, resulting in repression of PL and up-regulation of PR. At high concentrations Cro will bind to OR-Cro and repress PR. CI has high affinity binding to OR-CI, resulting in repression of PR and up-regulation of PL. At high concentrations CI will bind to OL-CI and repress PL. false false _1855_ 25667 25667 9 Not in stock false Divergent promoters where incorporated within the same part due to overlap of operators and promoters. false Thomas Overbeck annotation2431030 1 PR range2431030 1 64 92 annotation2431031 1 PL range2431031 1 7 36 annotation2431035 1 OR-CI range2431035 1 65 92 annotation2431034 1 OL-CI range2431034 1 17 44 annotation2431032 1 OL-CRO range2431032 1 5 33 annotation2431033 1 OR-CRO range2431033 1 40 78 BBa_M45801_sequence 1 aaacacattatataagaaaaaaccacgttttgcaaactttttttgtcaaatgacgtttttttcttgacaaaccacgaaaagtggactataattaact igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z