BBa_M45802
1
BBa_M45802
Mutated Tuc2009 bidirectional promoter for decreased CI sensitivity
2015-04-14T11:00:00Z
2015-05-08T01:14:09Z
Kenny JG, Leach S, Hoz AB, Venema G, Kok J, Fitzgerald GF, Nauta S, Alonso JC, Sinderen D (2006) Characterization of the lytic-lysogenic switch of the lactococcal bacteriophage Tuc2009. Virology 347:434-446
A modified bi-directional promoter from lactococcal bacteriophage Tuc2009 containing a bp mutation for decreased binding affinity of the CI repressor protein to the operator OL-CI at high CI concentrations. Allowing for continued up-regulated transcription from the leftward promoter (PL). See BBa_M45801 for detailed description of bi-directional promoter and intergenic switch region regulation with CI and Cro repressor proteins.
false
false
_1855_
0
25667
9
Not in stock
false
G to T change at bp 32 from BBa_M45801 on Fwd strand
false
Thomas Overbeck
annotation2431039
1
PR
range2431039
1
64
92
annotation2431043
1
OR-CI
range2431043
1
65
92
annotation2431037
1
G to T
range2431037
1
32
32
annotation2431040
1
OL-CRO
range2431040
1
5
33
annotation2431038
1
PL
range2431038
1
7
36
annotation2431041
1
OR-CRO
range2431041
1
40
78
annotation2431042
1
OL-CI
range2431042
1
17
44
BBa_M45802_sequence
1
aaacacattatataagaaaaaaccacgtttttcaaactttttttgtcaaatgacgtttttttcttgacaaaccacgaaaagtggactataattaact
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z