##gff-version 3
##sequence-region BBa_M45810 1 554
BBa_M45810	.	ribosome_entry_site	106	120	.	+	0	ID=BBa_B0030;Name=BBa_B0030
BBa_M45810	.	engineered_region	106	120	.	+	0	ID=annotation7025;Name=BBa_B0030;Parent=BBa_B0030
BBa_M45810	.	ribosome_entry_site	113	117	.	+	0	ID=annotation1702;Name=RBS;Parent=BBa_B0030
BBa_M45810	.	ribosome_entry_site	106	120	.	+	0	ID=annotation1701;Name=RBS-1\Strong;Parent=BBa_B0030
BBa_M45810	.	T7_RNA_Polymerase_Promoter	1	97	.	+	0	ID=BBa_M45802;Name=BBa_M45802
BBa_M45810	.	sequence_feature	40	78	.	+	0	ID=annotation2431041;Name=OR-CRO;Parent=BBa_M45802
BBa_M45810	.	sequence_alteration	32	32	.	+	0	ID=annotation2431037;Name=G to T;Parent=BBa_M45802
BBa_M45810	.	operator	65	92	.	+	0	ID=annotation2431043;Name=OR-CI;Parent=BBa_M45802
BBa_M45810	.	promoter	64	92	.	+	0	ID=annotation2431039;Name=PR;Parent=BBa_M45802
BBa_M45810	.	promoter	7	36	.	+	0	ID=annotation2431038;Name=PL;Parent=BBa_M45802
BBa_M45810	.	operator	5	33	.	+	0	ID=annotation2431040;Name=OL-CRO;Parent=BBa_M45802
BBa_M45810	.	operator	17	44	.	+	0	ID=annotation2431042;Name=OL-CI;Parent=BBa_M45802
BBa_M45810	.	terminator	516	554	.	+	0	ID=BBa_B1006;Name=BBa_B1006
BBa_M45810	.	polyA_site	547	554	.	+	0	ID=annotation1898430;Name=PolyA;Parent=BBa_B1006
BBa_M45810	.	polyA_site	516	524	.	+	0	ID=annotation1898431;Name=PolyA;Parent=BBa_B1006
BBa_M45810	.	engineered_region	516	554	.	+	0	ID=annotation1898428;Name=B1006;Parent=BBa_B1006
BBa_M45810	.	stem_loop	525	546	.	+	0	ID=annotation1898429;Name=modified thr terminator;Parent=BBa_B1006
BBa_M45810	.	sequence_feature	127	507	.	+	0	ID=BBa_M45805;Name=BBa_M45805
BBa_M45810	.	stop_codon	504	507	.	+	0	ID=annotation2431107;Name=Stop Codon;Parent=BBa_M45805
BBa_M45810	.	CDS	130	503	.	+	0	ID=annotation2431108;Name=Coding Sequence;Parent=BBa_M45805
BBa_M45810	.	start_codon	127	129	.	+	0	ID=annotation2431106;Name=Start Codon;Parent=BBa_M45805
>BBa_M45810
aaacacattatataagaaaaaaccacgtttttcaaactttttttgtcaaatgacgtttttttcttgacaaaccacgaaa
agtggactataattaacttactagagattaaagaggagaaatactagatgagcagccagcctgaaccaaagaagaaaaa
tctcgacgcgagcatcacaagcattatccatgaaatcggcgtcccagcccatattaaaggctatctctatctgcgcgaa
gcaatctcaatggtatacaatgacatcgaattgctcggcagcattacaaaagtcctctatccggacatcgccaaaaaat
ttaacacaaccgcaagccgtgtagaaagagcgatccgccatgcaattgaagtggcatggagcagaggaaacattgattc
catttcctcgttgtttggttatactgtcagcatgacaaaagctaaacctaccaacagtgaatttattgcaatggttgcg
gataagctgaggttagagcataaggcttcttaatactagagaaaaaaaaaccccgcccctgacagggcggggtttttttt
t