BBa_M45905 1 BBa_M45905 Composite part for fluorescent detection of botulinum neurotoxin A (BoNT A) 2015-04-12T11:00:00Z 2015-05-08T01:14:10Z SNAP-25: Homo sapiens GFP: Aequorea victoria Human neurotransmitter coding sequence (SNAP-25) targeted by botulinum neurotoxin A (BoNT) with green fluorescent protein (GFP) reporter and histadine tag for adhesion. false false _1855_ 0 25694 9 Not in stock false Optimized for expression in E. coli false Aaron Fronk component2430863 1 BBa_E0040 component2430870 1 BBa_K844000 component2430861 1 BBa_B0035 component2430866 1 BBa_M45904 annotation2430866 1 BBa_M45904 range2430866 1 747 917 annotation2430870 1 BBa_K844000 range2430870 1 926 961 annotation2430861 1 BBa_B0035 range2430861 1 1 14 annotation2430863 1 BBa_E0040 range2430863 1 21 740 BBa_K844000 1 BBa_K844000 10x-Histidine (10x-His) Tag with double stop codon (TAATAA) 2012-10-01T11:00:00Z 2015-05-08T01:13:33Z Constructed through oligonucleotide annealing Released HQ 2013 10x-Histidine tag with double stop codon TAATAA to allow for better extraction of tagged products and protein termination in a single part. false false _1104_ 0 9404 9 In stock true none false Kathleen Miller annotation2206607 1 10x-Histidine Tag range2206607 1 1 30 annotation2206608 1 Stop range2206608 1 31 33 annotation2206609 1 Stop range2206609 1 34 36 BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_M45904 1 BBa_M45904 SNAP-25 BoNT A cleavage site 2015-03-31T11:00:00Z 2015-05-08T01:14:10Z Homo sapiens genome The section of the human neurotransmitter protein SNAP-25 required for binding and cleavage by the botulinum neurotoxin A (BoNT), according to the findings of Vaidyanathan et al. (2002). false false _1855_ 0 25694 9 Not in stock false The sequence is optimized for expression in E. coli. false Aaron Fronk annotation2430850 1 Point mutation to make it RFC compatible range2430850 1 12 12 annotation2430847 1 End of sequence range2430847 1 171 171 annotation2430849 1 Start codon range2430849 1 1 3 BBa_M45905_sequence 1 attaaagaggagaatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagatggacgagaacctagaacaagtaagcgggatcattggcaatctgcgccatatggcactagatatgggtaacgagattgacactcagaaccgccaaattgatcgaataatggaaaaagcagattccaacaaaacgcgtattgacgaagcgaatcagcgcgctacaaagatgtactagagcatcatcaccatcaccaccatcatcaccattaataa BBa_M45904_sequence 1 atggacgagaacctagaacaagtaagcgggatcattggcaatctgcgccatatggcactagatatgggtaacgagattgacactcagaaccgccaaattgatcgaataatggaaaaagcagattccaacaaaacgcgtattgacgaagcgaatcagcgcgctacaaagatg BBa_B0035_sequence 1 attaaagaggagaa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_K844000_sequence 1 catcatcaccatcaccaccatcatcaccattaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z