LOCUS BBa_M45994 5790 bp DNA linear 26-Nov-2024 DEFINITION RBS and phasin ACCESSION BBa_M45994 VERSION BBa_M45994.1 FEATURES Location/Qualifiers promoter 1..94 /label=BBa_I721001 misc_feature 107..110 /label=conserved RBS 103..114 /label=BBa_B0034 CDS 121..806 /label=Coding Sequence misc_feature 121..123 /label=Start Codon misc_feature 804..806 /label=Stop Codon CDS 121..806 /label=BBa_M45995 misc_feature 819..822 /label=conserved RBS 815..826 /label=BBa_B0034 CDS 833..1518 /label=Coding Sequence misc_feature 833..835 /label=Start Codon misc_feature 1516..1518 /label=Stop Codon CDS 833..1518 /label=BBa_M45995 misc_feature 1531..1534 /label=conserved RBS 1527..1538 /label=BBa_B0034 CDS 1545..2230 /label=Coding Sequence misc_feature 1545..1547 /label=Start Codon misc_feature 2228..2230 /label=Stop Codon CDS 1545..2230 /label=BBa_M45995 misc_feature 2243..2246 /label=conserved RBS 2239..2250 /label=BBa_B0034 CDS 2257..2942 /label=Coding Sequence misc_feature 2257..2259 /label=Start Codon misc_feature 2940..2942 /label=Stop Codon CDS 2257..2942 /label=BBa_M45995 misc_feature 2955..2958 /label=conserved RBS 2951..2962 /label=BBa_B0034 CDS 2969..3654 /label=Coding Sequence misc_feature 2969..2971 /label=Start Codon misc_feature 3652..3654 /label=Stop Codon CDS 2969..3654 /label=BBa_M45995 misc_feature 3667..3670 /label=conserved RBS 3663..3674 /label=BBa_B0034 CDS 3681..4366 /label=Coding Sequence misc_feature 3681..3683 /label=Start Codon misc_feature 4364..4366 /label=Stop Codon CDS 3681..4366 /label=BBa_M45995 misc_feature 4379..4382 /label=conserved RBS 4375..4386 /label=BBa_B0034 CDS 4393..5078 /label=Coding Sequence misc_feature 4393..4395 /label=Start Codon misc_feature 5076..5078 /label=Stop Codon CDS 4393..5078 /label=BBa_M45995 misc_feature 5091..5094 /label=conserved RBS 5087..5098 /label=BBa_B0034 CDS 5105..5790 /label=Coding Sequence misc_feature 5105..5107 /label=Start Codon misc_feature 5788..5790 /label=Stop Codon CDS 5105..5790 /label=BBa_M45995 ORIGIN 1 ggttgcttcc tataaaaaac ttgactctat atctactaga ggttttctaa tgatggcatc 61 cggggaaaac cttgtcaatg aagagcgatc tatgtactag agaaagagga gaaatactag 121 atgatcctca ccccggaaca agttgcagca gcgcaaaagg ccaacctcga aacgctgttc 181 ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg tcgagctgaa cctacaggtc 241 gtcaagactt cgttcgcaga aggcgttgac aacgccaaga aggcgctgtc ggccaaggac 301 gcacaggaac tgctggccat ccaggccgca gccgtgcagc cggttgccga aaagaccctg 361 gcctacaccc gccacctgta tgaaatcgct tcggaaaccc agagcgagtt caccaaggta 421 gccgaggctc aactggccga aggctcgaag aacgtgcaag cgctggtcga gaacctcgcc 481 aagaacgccc cggccggttc ggaatcgacc gtggccatcg tgaagtcggc gatctccgct 541 gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc aagcggtcga aatcgctgaa 601 accaacttcc aggctgcggc tacggctgcc accaaggctg cccagcaagc cagcgccacg 661 gcccgtacgg ccacggcaaa gaagacgacg gctgcctgat aactgcctgc gttgaagatg 721 gaccggctgc ggccggtccg ttggcaaagc atatcgacgc ctggcgtttg cggtgtgttt 781 tgccaacgat gaaggtagtg ccctgatact agagaaagag gagaaatact agatgatcct 841 caccccggaa caagttgcag cagcgcaaaa ggccaacctc gaaacgctgt tcggcctgac 901 caccaaggcg tttgaaggcg tcgaaaagct cgtcgagctg aacctacagg tcgtcaagac 961 ttcgttcgca gaaggcgttg acaacgccaa gaaggcgctg tcggccaagg acgcacagga 1021 actgctggcc atccaggccg cagccgtgca gccggttgcc gaaaagaccc tggcctacac 1081 ccgccacctg tatgaaatcg cttcggaaac ccagagcgag ttcaccaagg tagccgaggc 1141 tcaactggcc gaaggctcga agaacgtgca agcgctggtc gagaacctcg ccaagaacgc 1201 cccggccggt tcggaatcga ccgtggccat cgtgaagtcg gcgatctccg ctgccaacaa 1261 cgcctacgag tcggtgcaga aggcgaccaa gcaagcggtc gaaatcgctg aaaccaactt 1321 ccaggctgcg gctacggctg ccaccaaggc tgcccagcaa gccagcgcca cggcccgtac 1381 ggccacggca aagaagacga cggctgcctg ataactgcct gcgttgaaga tggaccggct 1441 gcggccggtc cgttggcaaa gcatatcgac gcctggcgtt tgcggtgtgt tttgccaacg 1501 atgaaggtag tgccctgata ctagagaaag aggagaaata ctagatgatc ctcaccccgg 1561 aacaagttgc agcagcgcaa aaggccaacc tcgaaacgct gttcggcctg accaccaagg 1621 cgtttgaagg cgtcgaaaag ctcgtcgagc tgaacctaca ggtcgtcaag acttcgttcg 1681 cagaaggcgt tgacaacgcc aagaaggcgc tgtcggccaa ggacgcacag gaactgctgg 1741 ccatccaggc cgcagccgtg cagccggttg ccgaaaagac cctggcctac acccgccacc 1801 tgtatgaaat cgcttcggaa acccagagcg agttcaccaa ggtagccgag gctcaactgg 1861 ccgaaggctc gaagaacgtg caagcgctgg tcgagaacct cgccaagaac gccccggccg 1921 gttcggaatc gaccgtggcc atcgtgaagt cggcgatctc cgctgccaac aacgcctacg 1981 agtcggtgca gaaggcgacc aagcaagcgg tcgaaatcgc tgaaaccaac ttccaggctg 2041 cggctacggc tgccaccaag gctgcccagc aagccagcgc cacggcccgt acggccacgg 2101 caaagaagac gacggctgcc tgataactgc ctgcgttgaa gatggaccgg ctgcggccgg 2161 tccgttggca aagcatatcg acgcctggcg tttgcggtgt gttttgccaa cgatgaaggt 2221 agtgccctga tactagagaa agaggagaaa tactagatga tcctcacccc ggaacaagtt 2281 gcagcagcgc aaaaggccaa cctcgaaacg ctgttcggcc tgaccaccaa ggcgtttgaa 2341 ggcgtcgaaa agctcgtcga gctgaaccta caggtcgtca agacttcgtt cgcagaaggc 2401 gttgacaacg ccaagaaggc gctgtcggcc aaggacgcac aggaactgct ggccatccag 2461 gccgcagccg tgcagccggt tgccgaaaag accctggcct acacccgcca cctgtatgaa 2521 atcgcttcgg aaacccagag cgagttcacc aaggtagccg aggctcaact ggccgaaggc 2581 tcgaagaacg tgcaagcgct ggtcgagaac ctcgccaaga acgccccggc cggttcggaa 2641 tcgaccgtgg ccatcgtgaa gtcggcgatc tccgctgcca acaacgccta cgagtcggtg 2701 cagaaggcga ccaagcaagc ggtcgaaatc gctgaaacca acttccaggc tgcggctacg 2761 gctgccacca aggctgccca gcaagccagc gccacggccc gtacggccac ggcaaagaag 2821 acgacggctg cctgataact gcctgcgttg aagatggacc ggctgcggcc ggtccgttgg 2881 caaagcatat cgacgcctgg cgtttgcggt gtgttttgcc aacgatgaag gtagtgccct 2941 gatactagag aaagaggaga aatactagat gatcctcacc ccggaacaag ttgcagcagc 3001 gcaaaaggcc aacctcgaaa cgctgttcgg cctgaccacc aaggcgtttg aaggcgtcga 3061 aaagctcgtc gagctgaacc tacaggtcgt caagacttcg ttcgcagaag gcgttgacaa 3121 cgccaagaag gcgctgtcgg ccaaggacgc acaggaactg ctggccatcc aggccgcagc 3181 cgtgcagccg gttgccgaaa agaccctggc ctacacccgc cacctgtatg aaatcgcttc 3241 ggaaacccag agcgagttca ccaaggtagc cgaggctcaa ctggccgaag gctcgaagaa 3301 cgtgcaagcg ctggtcgaga acctcgccaa gaacgccccg gccggttcgg aatcgaccgt 3361 ggccatcgtg aagtcggcga tctccgctgc caacaacgcc tacgagtcgg tgcagaaggc 3421 gaccaagcaa gcggtcgaaa tcgctgaaac caacttccag gctgcggcta cggctgccac 3481 caaggctgcc cagcaagcca gcgccacggc ccgtacggcc acggcaaaga agacgacggc 3541 tgcctgataa ctgcctgcgt tgaagatgga ccggctgcgg ccggtccgtt ggcaaagcat 3601 atcgacgcct ggcgtttgcg gtgtgttttg ccaacgatga aggtagtgcc ctgatactag 3661 agaaagagga gaaatactag atgatcctca ccccggaaca agttgcagca gcgcaaaagg 3721 ccaacctcga aacgctgttc ggcctgacca ccaaggcgtt tgaaggcgtc gaaaagctcg 3781 tcgagctgaa cctacaggtc gtcaagactt cgttcgcaga aggcgttgac aacgccaaga 3841 aggcgctgtc ggccaaggac gcacaggaac tgctggccat ccaggccgca gccgtgcagc 3901 cggttgccga aaagaccctg gcctacaccc gccacctgta tgaaatcgct tcggaaaccc 3961 agagcgagtt caccaaggta gccgaggctc aactggccga aggctcgaag aacgtgcaag 4021 cgctggtcga gaacctcgcc aagaacgccc cggccggttc ggaatcgacc gtggccatcg 4081 tgaagtcggc gatctccgct gccaacaacg cctacgagtc ggtgcagaag gcgaccaagc 4141 aagcggtcga aatcgctgaa accaacttcc aggctgcggc tacggctgcc accaaggctg 4201 cccagcaagc cagcgccacg gcccgtacgg ccacggcaaa gaagacgacg gctgcctgat 4261 aactgcctgc gttgaagatg gaccggctgc ggccggtccg ttggcaaagc atatcgacgc 4321 ctggcgtttg cggtgtgttt tgccaacgat gaaggtagtg ccctgatact agagaaagag 4381 gagaaatact agatgatcct caccccggaa caagttgcag cagcgcaaaa ggccaacctc 4441 gaaacgctgt tcggcctgac caccaaggcg tttgaaggcg tcgaaaagct cgtcgagctg 4501 aacctacagg tcgtcaagac ttcgttcgca gaaggcgttg acaacgccaa gaaggcgctg 4561 tcggccaagg acgcacagga actgctggcc atccaggccg cagccgtgca gccggttgcc 4621 gaaaagaccc tggcctacac ccgccacctg tatgaaatcg cttcggaaac ccagagcgag 4681 ttcaccaagg tagccgaggc tcaactggcc gaaggctcga agaacgtgca agcgctggtc 4741 gagaacctcg ccaagaacgc cccggccggt tcggaatcga ccgtggccat cgtgaagtcg 4801 gcgatctccg ctgccaacaa cgcctacgag tcggtgcaga aggcgaccaa gcaagcggtc 4861 gaaatcgctg aaaccaactt ccaggctgcg gctacggctg ccaccaaggc tgcccagcaa 4921 gccagcgcca cggcccgtac ggccacggca aagaagacga cggctgcctg ataactgcct 4981 gcgttgaaga tggaccggct gcggccggtc cgttggcaaa gcatatcgac gcctggcgtt 5041 tgcggtgtgt tttgccaacg atgaaggtag tgccctgata ctagagaaag aggagaaata 5101 ctagatgatc ctcaccccgg aacaagttgc agcagcgcaa aaggccaacc tcgaaacgct 5161 gttcggcctg accaccaagg cgtttgaagg cgtcgaaaag ctcgtcgagc tgaacctaca 5221 ggtcgtcaag acttcgttcg cagaaggcgt tgacaacgcc aagaaggcgc tgtcggccaa 5281 ggacgcacag gaactgctgg ccatccaggc cgcagccgtg cagccggttg ccgaaaagac 5341 cctggcctac acccgccacc tgtatgaaat cgcttcggaa acccagagcg agttcaccaa 5401 ggtagccgag gctcaactgg ccgaaggctc gaagaacgtg caagcgctgg tcgagaacct 5461 cgccaagaac gccccggccg gttcggaatc gaccgtggcc atcgtgaagt cggcgatctc 5521 cgctgccaac aacgcctacg agtcggtgca gaaggcgacc aagcaagcgg tcgaaatcgc 5581 tgaaaccaac ttccaggctg cggctacggc tgccaccaag gctgcccagc aagccagcgc 5641 cacggcccgt acggccacgg caaagaagac gacggctgcc tgataactgc ctgcgttgaa 5701 gatggaccgg ctgcggccgg tccgttggca aagcatatcg acgcctggcg tttgcggtgt 5761 gttttgccaa cgatgaaggt agtgccctga //