BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M45999 1 BBa_M45999 demonstration 2014-03-23T12:00:00Z 2015-05-08T01:14:10Z E.coli genomic DNA this ie an example false false _1855_ 0 2425 303 Not in stock false removed restriction sites false Charles Miller annotation2371837 1 start range2371837 1 1 3 annotation2371839 1 PstI site range2371839 1 112 117 annotation2371838 1 stop range2371838 1 683 686 annotation2371836 1 cds range2371836 1 1 683 BBa_I721001 1 BBa_I721001 Lead Promoter 2007-10-25T11:00:00Z 2015-08-31T04:07:53Z ralstonia metallidurans Lead Promoter (Part 4) messed up EcoRI site false false _113_ 0 1531 58 It's complicated true none true Jeffrey Hofmann BBa_M45998 1 BBa_M45998 PhaP with RBS 2014-03-23T12:00:00Z 2015-05-08T01:14:10Z jadfs sdjfhadf false false _1855_ 0 2425 303 Not in stock false jdakdsj false Charles Miller component2371854 1 BBa_M45999 component2371847 1 BBa_I721001 component2371849 1 BBa_B0034 annotation2371854 1 BBa_M45999 range2371854 1 121 806 annotation2371849 1 BBa_B0034 range2371849 1 103 114 annotation2371847 1 BBa_I721001 range2371847 1 1 94 BBa_I721001_sequence 1 ggttgcttcctataaaaaacttgactctatatctactagaggttttctaatgatggcatccggggaaaaccttgtcaatgaagagcgatctatg BBa_B0034_sequence 1 aaagaggagaaa BBa_M45998_sequence 1 ggttgcttcctataaaaaacttgactctatatctactagaggttttctaatgatggcatccggggaaaaccttgtcaatgaagagcgatctatgtactagagaaagaggagaaatactagatgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctccaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga BBa_M45999_sequence 1 atgatcctcaccccggaacaagttgcagcagcgcaaaaggccaacctcgaaacgctgttcggcctgaccaccaaggcgtttgaaggcgtcgaaaagctcgtcgagctgaacctccaggtcgtcaagacttcgttcgcagaaggcgttgacaacgccaagaaggcgctgtcggccaaggacgcacaggaactgctggccatccaggccgcagccgtgcagccggttgccgaaaagaccctggcctacacccgccacctgtatgaaatcgcttcggaaacccagagcgagttcaccaaggtagccgaggctcaactggccgaaggctcgaagaacgtgcaagcgctggtcgagaacctcgccaagaacgccccggccggttcggaatcgaccgtggccatcgtgaagtcggcgatctccgctgccaacaacgcctacgagtcggtgcagaaggcgaccaagcaagcggtcgaaatcgctgaaaccaacttccaggctgcggctacggctgccaccaaggctgcccagcaagccagcgccacggcccgtacggccacggcaaagaagacgacggctgcctgataactgcctgcgttgaagatggaccggctgcggccggtccgttggcaaagcatatcgacgcctggcgtttgcggtgtgttttgccaacgatgaaggtagtgccctga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z