BBa_M47008 1 BBa_M47008 Time-Delay Input Filter 2015-05-12T11:00:00Z 2015-05-12T11:33:56Z Lac Operon Promoter from E. Coli Genome Hat4 gene and COX5B-1 promoter sequence from Arabidopsis thalian genome GFP from Aequorea victoria genome The first component in this part is an inducible promoter. This acts as the receiver for the input signal and can be modified according to the need of a system. In the sequence submitted, this is the Lac Operon Promoter. The promoter regulates the transcription of a weak inducer protein that must homodimerize in order to become active. The need for homodimerization introduces the time delay as the protein needs to build up for an extended period of time before becoming active. The exact protein chosen for this component can be varied depending on the delay needed. In the sequence submitted, this is HD-Zip family transcription factor Hat4. This transcription factor is followed by a transcriptional terminator sequence and then the promoter which our transcription factor activates; in this case COX5B-1. This secondary promoter directly regulates the part's output. This output is modular but in the submitted sequence it is GFP. false false _1987_ 26437 26437 9 false Strength of interaction between the homodimerizing inducer and its promoter will determine how long the output lags the input. Multiple combinations will likely need to be tested in order to achieve desired performance. false Julian Alverio annotation2432056 1 COX5B-1 range2432056 1 961 1851 annotation2432053 1 Lac Operon Promoter range2432053 1 1 37 annotation2432057 1 GFP range2432057 1 1862 2551 annotation2432055 1 Transcriptional Terminator range2432055 1 914 960 annotation2432054 1 Hat4 range2432054 1 47 913 BBa_M47008_sequence 1 tttacactttatgcttccggctcgtatgttgtgtggagaaagtgtatatgatgtttgaaaaagatgatctgggcctgagcctgggcctgaactttccgaaaaaacagattaacctgaaaagcaacccgagcgtgagcgtgaccccgagcagcagcagctttggcctgtttcgccgcagcagctggaacgaaagctttaccagcagcgtgccgaacagcgatagcagccagaaagaaacccgcacctttattcgcggcattgatgtgaaccgcccgccgagcaccgcggaatatggcgatgaagatgcgggcgtgagcagcccgaacagcaccgtgagcagcagcaccggcaaacgcagcgaacgcgaagaagataccgatccgcagggcagccgcggcattagcgatgatgaagatggcgataacagccgcaaaaaactgcgcctgagcaaagatcagagcgcgattctggaagaaacctttaaagatcatagcaccctgaacccgaaacagaaacaggcgctggcgaaacagctgggcctgcgcgcgcgccaggtggaagtgtggtttcagaaccgccgcgcgcgcaccaaactgaaacagaccgaagtggattgcgaatttctgcgccgctgctgcgaaaacctgaccgaagaaaaccgccgcctacagaaagaagtgaccgaactgcgcgcgctgaaactgagcccgcagttttatatgcatatgagcccgccgaccaccctgaccatgtgcccgagctgcgaacatgtgagcgtgccgccgccgcagccgcaggcggcgaccagcgcgcatcatcgcagcctgccggtgaacgcgtgggcgccggcgacccgcattagccatggcctgacctttgatgcgctgcgcccgcgcagctaaagagaatataaaaagccagattattaatccggcttttttattattttaaaaacatgtatcattaatttccactccaaatggcacacaacaatatagacacgtgcgcacacgtaccagcatcattgtatcattatttccacttgtattccacttgaaatgtcacacaatatagacacgtgtgcacacgtaccgatctaattcaggttctaacgttcgaaccctggcagcattaccatttatacagaacaagtagacacgtgcgctcacgtacgaatctaatgcaggttctaacgttcgaaccctggcagcattaccatttatacagaacaagtagacacgtgcgctcacgtacgaacaccataaacacatgatcgggttcaggttgagagtggcttagagagtggcttagagatacagtatatattaggcacgtgcgcacacgtacgaatgcaatttctcgaataatagtttaggtaattaagatgtataaaatcattactatcattcagacacgtgcgtacacgttaattcatcggcggaattttgttcagctgtataacttgtaatatacaccccatcttcaagtgggggggcacgtgcaatcacgtgctaagtgatgaaaaaaattgttgagtaaaattgcttaacacccgagttggagatcttattagtgaggcacgtgcacacacgtggcaaaatttgctcgtaaccatgaaatcagaacaaatgaagggtagaggaaagtgacttaatgacacgtgcgctcacgtgacagtaaaattaataataataataaaaaaaatctctacgcatgtatttggaaattttgaaaagtaaacacacgtggggacacgtgcaatttaaacaacgcattttttttaaaaagaaaatcgagaatgaggtttccaggaatggagcacacgtgcacacactgtgagaaagtgtatatgtcgtctggtgcactgttgtttcatggtaaaatcccgtatgttgtagaaatggaaggcaatgtcgatggtcacacctttagcattcgcggcaagggttacggtgacgcgagcgttggtaaggtcgacgcgcagtttatctgcaccacgggtgacgtcccggtgccgtggagcacgttggtgacgacgctgacttacggtgcccaatgtttcgcgaaatatggcccggggctgaaagacttctacaaatcctgtatgccggaaggttatgtgcaagagcgcactattacctttgagggtgacggtgtctttaagacccgtgccgaagtgaccttcgaaaacggtagcgtttacaaccgtgtcaagctgaatggccagggtttcaaaaaggatggtcacgttctgggtaagaatctggaactcaacttcaccccacactgcctgtacatctggggcgatcaagcgaatcatggtctgaaaagcgcatttaagatcatgcacgagattaccggctccaaagaagatttcatcgtggctgatcacacccagatgaataccccgattggcggtggccctgtgcacgttccggaatatcatcacctgaccgtctggacgagcttcggtaaggatccggatgacgacgaaaccgaccacctgaacatcgtagaggttatcaaagcagtggacctggagacgtatcgc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z