BBa_M50000 1 eCPX-nSA-c Streptavidin binding outer membrane protein 2016-10-23T11:00:00Z 2016-12-09T07:58:12Z Derived from E. coli outer membrane protein OmpX. eCPX is an engineered version of OmpX developed by Rice and Daugherty (Protein Eng. Des. Sel., 2008, pg 435-442) Cell surface display protein with N-terminal and C-terminal streptavidin binding domain. eCPX-nSA-cNano will be used to make E. coli bind to streptavidin coated magnetic beads, enabling bacterial capture. false false _848_ 34382 34382 9 false eCPX was developed as a biterminal display scaffold, and thus both N and C termini are available for fusion with other domains. An N terminal fusion with a streptavidin binding domain had already been created by Rice and Daugherty for their paper on eCPX; we wished to increase the affinity by fusing another (stronger binding) streptavidin binding domain to the C terminus false Nicolas Quach annotation2530174 1 Ser-Gly linker range2530174 1 617 634 annotation2530172 1 NanoTag range2530172 1 635 679 annotation2530173 1 SA-binding domain range2530173 1 86 130 BBa_M50000_sequence 1 atgaagaagattgcatgtctttcagcccttgctgccgtgttggccttcactgccggaacgagcgttgcaggccagtcaggtcaagcagagtgccatccccagggccccccttgcatcgaaggccgtaagggtggacaaagcggccagtcgggagattataacaaaaaccaatactacggtattacggctggaccagcataccgcatcaacgattgggcttctatttatggggtggttggagttggctatggtaaattccaaacgacagagtaccccacttataagcacgatacctctgattatgggttctcctacggcgcaggacttcagtttaatccgatggagaacgtagcattagatttctcgtatgagcagagtcgtatccgctcagtggacgttggtacttggatcttgtcagtggggtatcgcttcggctcgaagtctcgccgcgctacgtccactgtgacaggtggatatgcccagagcgacgcacaagggcagatgaacaaaatgggtggctttaaccttaagtaccgttatgaggaagataattcaccccttggggttatcggctcctttacctacacagaaaagtcccgcaccgcgagtgggggaagtggtggaggttcgggagatgttgaggcatggctgggagctcgcgtcccgcttgtagaaacctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z