BBa_M50003 1 BBa_M50003 StickyGreen 2016-10-24T11:00:00Z 2016-10-25T05:42:32Z N/A Bicistronic construct allowing linked expression of eCPX-nSA-cNano, a cell surface display protein containing two streptavidin binding domains, and mNeonGreen, a bright green fluorescent protein. A spacer separates the two genes. Both genes are preceded by a strong RBS. false false _848_ 34382 34382 9 false It is unclear whether a spacer is necessary to separate the two genes. We included the spacer sequence to ensure even expression of the two genes by preventing possible ribosome collision events. false Nicolas Quach component2529056 1 BBa_M50001 component2529059 1 BBa_M50002 component2529054 1 BBa_B0034 component2529058 1 BBa_B0034 component2529055 1 BBa_M50000 annotation2529059 1 BBa_M50002 range2529059 1 746 1456 annotation2529058 1 BBa_B0034 range2529058 1 728 739 annotation2529055 1 BBa_M50000 range2529055 1 19 681 annotation2529056 1 BBa_M50001 range2529056 1 690 719 annotation2529054 1 BBa_B0034 range2529054 1 1 12 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_M50000 1 eCPX-nSA-c Streptavidin binding outer membrane protein 2016-10-23T11:00:00Z 2016-12-09T07:58:12Z Derived from E. coli outer membrane protein OmpX. eCPX is an engineered version of OmpX developed by Rice and Daugherty (Protein Eng. Des. Sel., 2008, pg 435-442) Cell surface display protein with N-terminal and C-terminal streptavidin binding domain. eCPX-nSA-cNano will be used to make E. coli bind to streptavidin coated magnetic beads, enabling bacterial capture. false false _848_ 34382 34382 9 false eCPX was developed as a biterminal display scaffold, and thus both N and C termini are available for fusion with other domains. An N terminal fusion with a streptavidin binding domain had already been created by Rice and Daugherty for their paper on eCPX; we wished to increase the affinity by fusing another (stronger binding) streptavidin binding domain to the C terminus false Nicolas Quach annotation2530174 1 Ser-Gly linker range2530174 1 617 634 annotation2530173 1 SA-binding domain range2530173 1 86 130 annotation2530172 1 NanoTag range2530172 1 635 679 BBa_M50002 1 BBa_M50002 mNeonGreen 2016-10-23T11:00:00Z 2016-10-25T05:38:05Z Branchiostoma lanceolatum Full sequence for the ultra bright green fluorescent protein mNeonGreen. It is currently the brightest GFP. Codon optimized for E. coli K12. false false _848_ 34382 34382 9 false None. false Nicolas Quach annotation2530175 1 mNeonGreen range2530175 1 1 711 BBa_M50001 1 BBa_M50001 DNA spacer sequence 2016-10-23T11:00:00Z 2016-12-09T08:45:49Z Derived from the lambda phage genome. The 30 bp correspond to a portion of the untranslated region of the late transcript, between genes Q and S. A 30bp DNA spacer sequence. Used to prevent ribosome collision in bicistronic constructs. false false _848_ 34382 34382 9 false It is unclear whether spacer sequences are truly necessary to separate two genes, nor is it clear how long such a sequence would need to be. false Nicolas Quach annotation2532379 1 spacer range2532379 1 1 15 BBa_M50000_sequence 1 atgaagaagattgcatgtctttcagcccttgctgccgtgttggccttcactgccggaacgagcgttgcaggccagtcaggtcaagcagagtgccatccccagggccccccttgcatcgaaggccgtaagggtggacaaagcggccagtcgggagattataacaaaaaccaatactacggtattacggctggaccagcataccgcatcaacgattgggcttctatttatggggtggttggagttggctatggtaaattccaaacgacagagtaccccacttataagcacgatacctctgattatgggttctcctacggcgcaggacttcagtttaatccgatggagaacgtagcattagatttctcgtatgagcagagtcgtatccgctcagtggacgttggtacttggatcttgtcagtggggtatcgcttcggctcgaagtctcgccgcgctacgtccactgtgacaggtggatatgcccagagcgacgcacaagggcagatgaacaaaatgggtggctttaaccttaagtaccgttatgaggaagataattcaccccttggggttatcggctcctttacctacacagaaaagtcccgcaccgcgagtgggggaagtggtggaggttcgggagatgttgaggcatggctgggagctcgcgtcccgcttgtagaaacctaa BBa_B0034_sequence 1 aaagaggagaaa BBa_M50003_sequence 1 aaagaggagaaatactagatgaagaagattgcatgtctttcagcccttgctgccgtgttggccttcactgccggaacgagcgttgcaggccagtcaggtcaagcagagtgccatccccagggccccccttgcatcgaaggccgtaagggtggacaaagcggccagtcgggagattataacaaaaaccaatactacggtattacggctggaccagcataccgcatcaacgattgggcttctatttatggggtggttggagttggctatggtaaattccaaacgacagagtaccccacttataagcacgatacctctgattatgggttctcctacggcgcaggacttcagtttaatccgatggagaacgtagcattagatttctcgtatgagcagagtcgtatccgctcagtggacgttggtacttggatcttgtcagtggggtatcgcttcggctcgaagtctcgccgcgctacgtccactgtgacaggtggatatgcccagagcgacgcacaagggcagatgaacaaaatgggtggctttaaccttaagtaccgttatgaggaagataattcaccccttggggttatcggctcctttacctacacagaaaagtcccgcaccgcgagtgggggagatgttgaggcatggctgggagctcgcgtcccgcttgtagaaacctaatactagagcatatcggtcacgaacaaatctgattactatactagagaaagaggagaaatactagatggtgtcaaaaggcgaggaggataatatggcctcgcttccagcgacgcacgagttgcatattttcggatctattaacggtgttgattttgacatggtaggtcagggcacggggaaccccaatgatggctatgaggaattaaatctgaaatcgacaaaaggggatttgcagttctccccatggatcttagttccacacatcggatatggcttccaccaatacttgccgtaccctgatgggatgtctccttttcaggcggccatggtggacggatctggctaccaagtacaccgtacaatgcaattcgaagatggtgcctcgttgaccgtaaactatcgttacacttacgaaggttcacacatcaagggagaagcccaggtaaagggcactggtttcccagccgatggccctgtcatgactaattctttgaccgcagcagattggtgccgttccaagaaaacctatccgaacgacaagacgatcatttcaacgttcaaatggagctacacgaccggaaacggaaagcgttatcgctctactgctcgtactacctacacatttgccaaaccgatggccgctaattatttaaaaaaccagcctatgtatgttttccgtaaaaccgagttgaaacactccaaaacagaacttaattttaaggagtggcagaaggcttttactgacgtaatgggtatggatgagttatataagtaa BBa_M50001_sequence 1 catatcggtcacgaa BBa_M50002_sequence 1 atggtgtcaaaaggcgaggaggataatatggcctcgcttccagcgacgcacgagttgcatattttcggatctattaacggtgttgattttgacatggtaggtcagggcacggggaaccccaatgatggctatgaggaattaaatctgaaatcgacaaaaggggatttgcagttctccccatggatcttagttccacacatcggatatggcttccaccaatacttgccgtaccctgatgggatgtctccttttcaggcggccatggtggacggatctggctaccaagtacaccgtacaatgcaattcgaagatggtgcctcgttgaccgtaaactatcgttacacttacgaaggttcacacatcaagggagaagcccaggtaaagggcactggtttcccagccgatggccctgtcatgactaattctttgaccgcagcagattggtgccgttccaagaaaacctatccgaacgacaagacgatcatttcaacgttcaaatggagctacacgaccggaaacggaaagcgttatcgctctactgctcgtactacctacacatttgccaaaccgatggccgctaattatttaaaaaaccagcctatgtatgttttccgtaaaaccgagttgaaacactccaaaacagaacttaattttaaggagtggcagaaggcttttactgacgtaatgggtatggatgagttatataagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z