BBa_M50015
1
BBa_M50015
Positive feedback cusR producer (E. coli)
2016-10-26T11:00:00Z
2016-12-01T12:19:13Z
RBS: BBa_B0035 http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection
cusR promoter from E.Coli K12 (250 nts before cusR protein sequence): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4
cusR from E.Coli K12 (684 nts of protein): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4
This sequence contains BsaI restriction enzyme sites on both ends. Following the BsaI restriction site on the 5' end, there is the promoter activated by cusR, followed by a high copy number RBS, followed by the sequence for cusR with STOP sequence at the end. Finally, there is the complementary BsaI restriction site.
false
false
_848_
34381
34400
9
false
Designed to be grafted onto EColi_Promoter_Casette with high copy number RBS and GFP
false
Derek Wang, Anika Naidu
component2530571
1
BBa_M50022
component2530570
1
BBa_B0035
component2530569
1
BBa_M50014
annotation2530569
1
BBa_M50014
range2530569
1
1
250
annotation2530571
1
BBa_M50022
range2530571
1
279
962
annotation2530570
1
BBa_B0035
range2530570
1
259
272
BBa_M50022
1
BBa_M50022
cusR protein (E. Coli)
2016-10-26T11:00:00Z
2016-11-30T10:30:17Z
from E. Coli str. K-12 substr. MG1655 (from http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4)
long
false
false
_848_
34381
34381
9
false
none
false
Anika Naidu
BBa_B0035
1
BBa_B0035
RBS (B0030 derivative)
2004-01-27T12:00:00Z
2015-08-31T04:07:20Z
-- No description --
false
false
_1_
0
24
7
Not in stock
false
false
Jason Kelly
BBa_M50014
1
BBa_M50014
cusR promoter (E. coli)
2016-10-26T11:00:00Z
2016-12-01T12:13:31Z
The 250 nucleotides before the cusR protein in E. Coli str. K-12 substr. MG1655 (from: http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=0)
long
false
false
_848_
34381
34381
9
false
none.
false
Anika Naidu, Derek Wang
BBa_M50014_sequence
1
gcacgggcattgccggacgctgataatccggtgccagtgaacaaccggttagcgcaagggccacacaaaatggcagaagtttacaaggagacataggctcataatttctggtgattttatgccgccaactttactcgccaggctctgattttccggtgacagaaaaatgacaaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgggcaacatgcggaggaaat
BBa_B0035_sequence
1
attaaagaggagaa
BBa_M50015_sequence
1
gcacgggcattgccggacgctgataatccggtgccagtgaacaaccggttagcgcaagggccacacaaaatggcagaagtttacaaggagacataggctcataatttctggtgattttatgccgccaactttactcgccaggctctgattttccggtgacagaaaaatgacaaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgggcaacatgcggaggaaattactagagattaaagaggagaatactagatgaaactgttgattgtcgaagatgaaaagaaaaccggagaatacttgaccaaagggttaaccgaagccggttttgtggtcgatttggccgacaacgggctgaatggctaccatctggcgatgaccggtgattatgatctgataatcctcgatattatgctgccggacgtgaacggctgggatatcgtgcgcatgttacgctccgccaataaagggatgccgattctgttgcttaccgcgcttggcaccattgaacatcgcgtcaaggggctggagttgggggcagatgactacctggtgaagccattcgcttttgctgaactgctggcgcgggtgcgcacattactgcggcgcggggcggcggtgattatcgaaagtcagtttcaggttgccgatttgatggtcgatctcgtcagccgcaaagtcacccgcagcggcacgcgcatcactttgaccagtaaagagtttactctgctggagttcttccttcgccatcagggcgaagtgctgccccgctcgcttatcgcctcgcaggtatgggacatgaattttgacagcgataccaatgctattgatgtggcggtgaagcggctgcgcggcaaaatcgacaacgactttgagccgaagctaattcagaccgtgcgcggcgtgggttacatgcttgaggtgccggatggtcagtaa
BBa_M50022_sequence
1
atgaaactgttgattgtcgaagatgaaaagaaaaccggagaatacttgaccaaagggttaaccgaagccggttttgtggtcgatttggccgacaacgggctgaatggctaccatctggcgatgaccggtgattatgatctgataatcctcgatattatgctgccggacgtgaacggctgggatatcgtgcgcatgttacgctccgccaataaagggatgccgattctgttgcttaccgcgcttggcaccattgaacatcgcgtcaaggggctggagttgggggcagatgactacctggtgaagccattcgcttttgctgaactgctggcgcgggtgcgcacattactgcggcgcggggcggcggtgattatcgaaagtcagtttcaggttgccgatttgatggtcgatctcgtcagccgcaaagtcacccgcagcggcacgcgcatcactttgaccagtaaagagtttactctgctggagttcttccttcgccatcagggcgaagtgctgccccgctcgcttatcgcctcgcaggtatgggacatgaattttgacagcgataccaatgctattgatgtggcggtgaagcggctgcgcggcaaaatcgacaacgactttgagccgaagctaattcagaccgtgcgcggcgtgggttacatgcttgaggtgccggatggtcagtaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z