BBa_M50015 1 BBa_M50015 Positive feedback cusR producer (E. coli) 2016-10-26T11:00:00Z 2016-12-01T12:19:13Z RBS: BBa_B0035 http://parts.igem.org/Ribosome_Binding_Sites/Prokaryotic/Constitutive/Community_Collection cusR promoter from E.Coli K12 (250 nts before cusR protein sequence): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4 cusR from E.Coli K12 (684 nts of protein): http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4 This sequence contains BsaI restriction enzyme sites on both ends. Following the BsaI restriction site on the 5' end, there is the promoter activated by cusR, followed by a high copy number RBS, followed by the sequence for cusR with STOP sequence at the end. Finally, there is the complementary BsaI restriction site. false false _848_ 34381 34400 9 false Designed to be grafted onto EColi_Promoter_Casette with high copy number RBS and GFP false Derek Wang, Anika Naidu component2530571 1 BBa_M50022 component2530570 1 BBa_B0035 component2530569 1 BBa_M50014 annotation2530569 1 BBa_M50014 range2530569 1 1 250 annotation2530571 1 BBa_M50022 range2530571 1 279 962 annotation2530570 1 BBa_B0035 range2530570 1 259 272 BBa_M50022 1 BBa_M50022 cusR protein (E. Coli) 2016-10-26T11:00:00Z 2016-11-30T10:30:17Z from E. Coli str. K-12 substr. MG1655 (from http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=4) long false false _848_ 34381 34381 9 false none false Anika Naidu BBa_B0035 1 BBa_B0035 RBS (B0030 derivative) 2004-01-27T12:00:00Z 2015-08-31T04:07:20Z -- No description -- false false _1_ 0 24 7 Not in stock false false Jason Kelly BBa_M50014 1 BBa_M50014 cusR promoter (E. coli) 2016-10-26T11:00:00Z 2016-12-01T12:13:31Z The 250 nucleotides before the cusR protein in E. Coli str. K-12 substr. MG1655 (from: http://microbesonline.org/cgi-bin/fetchLocus.cgi?locus=14707&disp=0) long false false _848_ 34381 34381 9 false none. false Anika Naidu, Derek Wang BBa_M50014_sequence 1 gcacgggcattgccggacgctgataatccggtgccagtgaacaaccggttagcgcaagggccacacaaaatggcagaagtttacaaggagacataggctcataatttctggtgattttatgccgccaactttactcgccaggctctgattttccggtgacagaaaaatgacaaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgggcaacatgcggaggaaat BBa_B0035_sequence 1 attaaagaggagaa BBa_M50015_sequence 1 gcacgggcattgccggacgctgataatccggtgccagtgaacaaccggttagcgcaagggccacacaaaatggcagaagtttacaaggagacataggctcataatttctggtgattttatgccgccaactttactcgccaggctctgattttccggtgacagaaaaatgacaaaattgtcattttgccaataagcgattgccatctgatcccgctactctagaattgcccgggcaacatgcggaggaaattactagagattaaagaggagaatactagatgaaactgttgattgtcgaagatgaaaagaaaaccggagaatacttgaccaaagggttaaccgaagccggttttgtggtcgatttggccgacaacgggctgaatggctaccatctggcgatgaccggtgattatgatctgataatcctcgatattatgctgccggacgtgaacggctgggatatcgtgcgcatgttacgctccgccaataaagggatgccgattctgttgcttaccgcgcttggcaccattgaacatcgcgtcaaggggctggagttgggggcagatgactacctggtgaagccattcgcttttgctgaactgctggcgcgggtgcgcacattactgcggcgcggggcggcggtgattatcgaaagtcagtttcaggttgccgatttgatggtcgatctcgtcagccgcaaagtcacccgcagcggcacgcgcatcactttgaccagtaaagagtttactctgctggagttcttccttcgccatcagggcgaagtgctgccccgctcgcttatcgcctcgcaggtatgggacatgaattttgacagcgataccaatgctattgatgtggcggtgaagcggctgcgcggcaaaatcgacaacgactttgagccgaagctaattcagaccgtgcgcggcgtgggttacatgcttgaggtgccggatggtcagtaa BBa_M50022_sequence 1 atgaaactgttgattgtcgaagatgaaaagaaaaccggagaatacttgaccaaagggttaaccgaagccggttttgtggtcgatttggccgacaacgggctgaatggctaccatctggcgatgaccggtgattatgatctgataatcctcgatattatgctgccggacgtgaacggctgggatatcgtgcgcatgttacgctccgccaataaagggatgccgattctgttgcttaccgcgcttggcaccattgaacatcgcgtcaaggggctggagttgggggcagatgactacctggtgaagccattcgcttttgctgaactgctggcgcgggtgcgcacattactgcggcgcggggcggcggtgattatcgaaagtcagtttcaggttgccgatttgatggtcgatctcgtcagccgcaaagtcacccgcagcggcacgcgcatcactttgaccagtaaagagtttactctgctggagttcttccttcgccatcagggcgaagtgctgccccgctcgcttatcgcctcgcaggtatgggacatgaattttgacagcgataccaatgctattgatgtggcggtgaagcggctgcgcggcaaaatcgacaacgactttgagccgaagctaattcagaccgtgcgcggcgtgggttacatgcttgaggtgccggatggtcagtaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z