BBa_M50019 1 BBa_M50019 Strong ribosome binding site 2016-10-26T11:00:00Z 2016-10-27T06:54:48Z false false _848_ 34405 34405 9 false false Taylor Merkel, Chloe Thai, Julia Schulz BBa_M50017 1 BBa_M50017 d-Limonene Synthase 2016-10-26T11:00:00Z 2016-10-27T06:55:12Z Optimized for e. coli based on https://www.ncbi.nlm.nih.gov/nuccore/AB110636.1 and BBa_K1660003 Encodes d-Limonene synthase, which catalyzes production of d-limonene (with citrus/orange scent) from GPP (Geranyl pyrophosphate) false false _848_ 34405 34405 9 false false Taylor Merkel, Chloe Thai, Julia Schulz BBa_M50049 1 BBa_M50049 Transcription terminator 2016-12-10T12:00:00Z 2016-12-11T01:06:34Z Sourced from bacteriophage T7 Transcription terminator false false _848_ 34405 34405 9 false No modifications false Taylor Merkel BBa_M50021 1 BBa_M50021 Heat-inducible d-Limonene synthase 2016-10-26T11:00:00Z 2016-12-11T09:46:42Z false false _848_ 34405 34405 9 false false Taylor Merkel, Chloe Thai, Julia Schulz component2532499 1 BBa_M50018 component2532502 1 BBa_M50049 component2532500 1 BBa_M50019 component2532501 1 BBa_M50017 annotation2532501 1 BBa_M50017 range2532501 1 67 1887 annotation2532502 1 BBa_M50049 range2532502 1 1896 1943 annotation2532500 1 BBa_M50019 range2532500 1 46 60 annotation2532499 1 BBa_M50018 range2532499 1 1 37 BBa_M50018 1 BBa_M50018 Sigma-32 Induced (Heat sensitive) Promoter 2016-10-26T11:00:00Z 2016-12-11T09:47:06Z Promoter sequence based on GroE gene promoter (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC193967/figure/f1/, http://www.sciencedirect.com/science/article/pii/037811199490846X) Promoter which binds preferentially to RNA polymerase holoenzyme containing sigma factor 32, which is upregulated with heat false false _848_ 34405 34405 9 false false Taylor Merkel, Chloe Thai, Julia Schulz BBa_M50049_sequence 1 ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_M50017_sequence 1 atgagtagttgtatcaacccgagcaccctggtgacaagcgtgaatggcttcaaatgcctgccgctggccaccaacaaggcagcaatccgcatcatggccaaaaacaagcctgtgcagtgcctggttagtgccaagtatgacaacctgaccgtggatcgccgcagtgcaaactaccagccgagcatttgggaccacgattttttacaaagcctgaacagcaactacaccgacgaaacctacaaacgccgtgaagaagagctgaaaggtaaagtgatgacaacaatcaaagatgtgaccgaacctctgaaccagctggagctgattgacagcttacaacgtctgggtctggcctaccactttgaaacagagatccgcaatatcctgcacgatatttataacagcaataacgactacgtgtggcgtaaagaaaacctgtacgccaccagcctggaatttcgcctgctgcgtcaacacggctatccggttagccaagaagtttttaacggcttcaaggacgatcagggcggcttcatttgcgacgatttcaaaggcgttctgagtctgcacgaagccagctacttcagcctggaaggtgagagcatcatggaagaggcctggcagttcaccagcaaacacctgaaagaggttatgattagcaaaagcaaacagggtgatgtgtttgttgccgagcaggcaaagcgtggcctggagctgccgctgcattggaaagtgcctatgctggaggcacgttggttcatcgatgtgtacgaaaagcgcgaggacaaaaatcacctgctgctggagttagccaagctggagtttaacgtgttacaggcaatctaccaagaagaactgaaggacgtgagtcgctggtggaaggatattggcctgggcgaaaaactgagctttgcccgcgatagtttagtggccagcttcgtgtggagcatgggcatcgtgtttgaaccgcagttcgcctattgccgccgtatcctgacaattacctttgccctgattagtgtgattgacgatatttacgacgtgtacggcaccctggatgaactggaactgttcgccgacgccgtggaacgttgggacattaactatgcattaaaccatctgccggactatatgaaaatctgctttctggccctgtacaatctggtgaacgagtttacatattacgtgctgaaacagcaggacttcgatatcctgcgtagcatcaaaaacgcctggctgcgcaatatccaggcctacctggtggaagcaaagtggtaccacggtaagtacacaccgaccctgggtgaatttctggagaacggtctggtgagcattggtggcccgatggtgacaatgacagcctatctgagcggtacaaacccgatcatcgagaaagagctggagttcctggagagtaaccaagatattagtcattggagcttcaaaattctgcgcttacaagatgacttaggtaccagcagtgacgagatccgccgtggcgatgttccgaagagcatccagtgttacatgcacgagaccggtgcaagcgaagaagttgcacgtgagcacattaaggacatgatgcgccagatgtggaaaaaggtgaatgcctaccgcgccgataaagatttccctctgagccagaccaccgtggagtttattctgaacgttgttcgcgttagtcatttcatgtatctgcatggcgacggccatggtgcccagaatcaggaaaccatggacgtggtttttaccctgctgttccaaccgatccctctggacgacaagcacatcgtggccacaagtagcccggttacaaaaggctaa BBa_M50021_sequence 1 tttcccccttgaaggggcgaagcctcatccccatttatactagagttaggaggtaaacattactagatgagtagttgtatcaacccgagcaccctggtgacaagcgtgaatggcttcaaatgcctgccgctggccaccaacaaggcagcaatccgcatcatggccaaaaacaagcctgtgcagtgcctggttagtgccaagtatgacaacctgaccgtggatcgccgcagtgcaaactaccagccgagcatttgggaccacgattttttacaaagcctgaacagcaactacaccgacgaaacctacaaacgccgtgaagaagagctgaaaggtaaagtgatgacaacaatcaaagatgtgaccgaacctctgaaccagctggagctgattgacagcttacaacgtctgggtctggcctaccactttgaaacagagatccgcaatatcctgcacgatatttataacagcaataacgactacgtgtggcgtaaagaaaacctgtacgccaccagcctggaatttcgcctgctgcgtcaacacggctatccggttagccaagaagtttttaacggcttcaaggacgatcagggcggcttcatttgcgacgatttcaaaggcgttctgagtctgcacgaagccagctacttcagcctggaaggtgagagcatcatggaagaggcctggcagttcaccagcaaacacctgaaagaggttatgattagcaaaagcaaacagggtgatgtgtttgttgccgagcaggcaaagcgtggcctggagctgccgctgcattggaaagtgcctatgctggaggcacgttggttcatcgatgtgtacgaaaagcgcgaggacaaaaatcacctgctgctggagttagccaagctggagtttaacgtgttacaggcaatctaccaagaagaactgaaggacgtgagtcgctggtggaaggatattggcctgggcgaaaaactgagctttgcccgcgatagtttagtggccagcttcgtgtggagcatgggcatcgtgtttgaaccgcagttcgcctattgccgccgtatcctgacaattacctttgccctgattagtgtgattgacgatatttacgacgtgtacggcaccctggatgaactggaactgttcgccgacgccgtggaacgttgggacattaactatgcattaaaccatctgccggactatatgaaaatctgctttctggccctgtacaatctggtgaacgagtttacatattacgtgctgaaacagcaggacttcgatatcctgcgtagcatcaaaaacgcctggctgcgcaatatccaggcctacctggtggaagcaaagtggtaccacggtaagtacacaccgaccctgggtgaatttctggagaacggtctggtgagcattggtggcccgatggtgacaatgacagcctatctgagcggtacaaacccgatcatcgagaaagagctggagttcctggagagtaaccaagatattagtcattggagcttcaaaattctgcgcttacaagatgacttaggtaccagcagtgacgagatccgccgtggcgatgttccgaagagcatccagtgttacatgcacgagaccggtgcaagcgaagaagttgcacgtgagcacattaaggacatgatgcgccagatgtggaaaaaggtgaatgcctaccgcgccgataaagatttccctctgagccagaccaccgtggagtttattctgaacgttgttcgcgttagtcatttcatgtatctgcatggcgacggccatggtgcccagaatcaggaaaccatggacgtggtttttaccctgctgttccaaccgatccctctggacgacaagcacatcgtggccacaagtagcccggttacaaaaggctaatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttg BBa_M50019_sequence 1 ttaggaggtaaacat BBa_M50018_sequence 1 tttcccccttgaaggggcgaagcctcatccccattta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z