BBa_M50032
1
BBa_M50032
pT-T7 terminator
2016-11-01T12:00:00Z
2016-11-01T06:29:47Z
Gene Designer 2.0
Generic bacteriophage T7 terminator obtained from Gene Designer 2.0.
false
false
_848_
34410
34409
9
false
None.
false
Cale Lester, Raquel Freeman, Tristan Yeung
BBa_M50029
1
BBa_M50029
mTurquoise2 FP
2016-11-01T12:00:00Z
2016-11-01T06:29:00Z
Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.
Fluorescent protein
excitation wavelength= 434 nm; emission wavelength=474 nm
Excitation state does not overlap with those of mKO2 FP and Clover FP
false
false
_848_
34410
34409
9
false
No BsaI restriction sites
false
Cale Lester, Raquel Freeman, Tristan Yeung
BBa_M50033
1
BBa_M50033
U1_mTurquoise2, IPTG-induced, CAM resistant Thermosensor Construct
2016-11-01T12:00:00Z
2016-12-11T07:37:42Z
Neupert, J., Karcher, D., and Bock, R. 2008. Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli. Nucleic Acids Research. 36(19): e124.
Bajar, B., et al. 2016. Fluorescent indicators for simultaneous reporting of all four cell cycle phases. Nature Methods.
At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. U1 has a unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. It contains a pP-T5 promoter, U1 RBS, mTurquoise2, and a pT-T7 terminator.
false
false
_848_
34409
34409
9
false
No BsaI restriction sites. Under 2kb for synthesis using DNA 2.0.
false
Cale Lester, Raquel Freeman, Tristan Yeung
component2531617
1
BBa_M50031
component2531618
1
BBa_M50007
component2531620
1
BBa_M50032
component2531619
1
BBa_M50029
annotation2531617
1
BBa_M50031
range2531617
1
1
48
annotation2531619
1
BBa_M50029
range2531619
1
87
806
annotation2531620
1
BBa_M50032
range2531620
1
807
854
annotation2531618
1
BBa_M50007
range2531618
1
49
86
BBa_M50031
1
BBa_M50031
pP-T5 promoter
2016-11-01T12:00:00Z
2016-11-01T06:29:33Z
Gene Designer 2.0
Generic E. coli T5 promoter obtained from Gene Designer 2.0
false
false
_848_
34410
34409
9
false
None.
false
Cale Lester, Raquel Freeman, Tristan Yeung
BBa_M50007
1
BBa_M50007
U1 Thermosensitive 5'-UTR (includes RBS)
2016-10-31T12:00:00Z
2016-12-11T08:40:03Z
Our unique parts are based off of E. coli thermosensors designed by Neupert et. al.
It utilizes the distinct thermosensor U1, with different expression patterns at various temperature levels.
At elevated temperatures, the U1 thermosensor releases its hairpin structure and allows the RBS to be readily accessible to the ribosome, enabling increased expression of our desired gene. We picked U1 because of its unique trait that allows for the detection of increasing gene expression levels ranging from 22˚C to 37˚C. We did not make any modifications to the thermosensor genetic sequences given by Neupert et. al.
Our plasmid includes a standard selectable marker, chloramphenicol, pP-T5 promoter, the U1 RBS, mTurquoise FP, pT-T7 terminator, and a high copy ORI.
false
false
_848_
34410
34409
9
false
Less than 2kb for DNA 2.0 to synthesize. No Bsa I restriction site sequence. No palindromic repeats longer than 5 bp or repeating nucleotides longer than approximately 10 bp.
false
Cale Lester, Raquel Freeman, Tristan Yeung
BBa_M50033_sequence
1
aaatcatgaaaaatttatttgctttgtgagcggataacaattataataggatcccaccttactagtctgcagaaggagatatacccatggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgtcctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactactttagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaactagcataaccccttggggcctctaaacgggtcttgaggggttttttg
BBa_M50029_sequence
1
atggtgagcaagggcgaggagctgttcaccggggtggtgcccatcctggtcgagctggacggcgacgtaaacggccacaagttcagcgtgtccggcgagggcgagggcgatgccacctacggcaagctgaccctgaagttcatctgcaccaccggcaagctgcccgtgccctggcccaccctcgtgaccaccctgtcctggggcgtgcagtgcttcgcccgctaccccgaccacatgaagcagcacgacttcttcaagtccgccatgcccgaaggctacgtccaggagcgcaccatcttcttcaaggacgacggcaactacaagacccgcgccgaggtgaagttcgagggcgacaccctggtgaaccgcatcgagctgaagggcatcgacttcaaggaggacggcaacatcctggggcacaagctggagtacaactactttagcgacaacgtctatatcaccgccgacaagcagaagaacggcatcaaggccaacttcaagatccgccacaacatcgaggacggcggcgtgcagctcgccgaccactaccagcagaacacccccatcggcgacggccccgtgctgctgcccgacaaccactacctgagcacccagtccaagctgagcaaagaccccaacgagaagcgcgatcacatggtcctgctggagttcgtgaccgccgccgggatcactctcggcatggacgagctgtacaagtaa
BBa_M50007_sequence
1
ggatcccaccttactagtctgcagaaggagatataccc
BBa_M50031_sequence
1
aaatcatgaaaaatttatttgctttgtgagcggataacaattataata
BBa_M50032_sequence
1
ctagcataaccccttggggcctctaaacgggtcttgaggggttttttg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z