BBa_M50036 1 BBa_M50036 6 Amino Acid Fusion Protein Linker 2016-12-11T12:00:00Z 2016-12-11T04:55:01Z Linker sequence was suggested and provided by our TA, Salil Bahte, and then optimized for use in E. coli. This sequence encodes for six amino acids that constitute the fusion protein linker for our Stanford BioE44 DNA construct. It is optimized for use in E. coli, and is designed to maintain linearity of the sequence and prevent protein folding. Our design relied on proper orientation of two GBP domains linked on each end to a flurophore. The linkers were included to ensure that glucose-binding caused the conformational change characterized with FRET instead of inherently folded protein structure, the linkers were included. false false _848_ 34390 34390 9 false Codon optimization software was used since we transformed our plasmid into E. coli. Also, the linker needed to be relatively short since we were already designing a long construct and needed to minimize the synthesis time given class constraints. false Amanda Urke BBa_M50036_sequence 1 gggggcaccggcgggtcc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z