LOCUS       BBa_M50047              2152 bp    DNA     linear       23-Dec-2024
DEFINITION  Linear DNA to be used with HIV I Integrase pD649
ACCESSION   BBa_M50047
VERSION     BBa_M50047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..40
                     /label=BBa_M50043
     promoter        49..845
                     /label=hCMV promoter
     promoter        1082..1100
                     /label=T7 Promoter
     misc_feature    1101..1109
                     /label=Kozak
     misc_feature    1110..1112
                     /label=Start
     promoter        49..1112
                     /label=BBa_M50045
     misc_feature    1829..1829
                     /label=Deletion of stop codon to integrate subsequent 
                     parts of construct
     misc_feature    1119..1829
                     /label=BBa_M50009
     misc_feature    1983..2104
                     /label=SV40 polyA terminator
     terminator      1838..2104
                     /label=BBa_M50046
     misc_feature    2113..2152
                     /label=BBa_M50044
ORIGIN
        1 actggaaggg ctaattcact cccaaagaag acaagatatc tactagagtc aatattggcc
       61 attagccata ttattcattg gttatatagc ataaatcaat attggctatt ggccattgca
      121 tacgttgtat ctatatcata atatgtacat ttatattggc tcatgtccaa tatgaccgcc
      181 atgttggcat tgattattga ctagttatta atagtaatca attacggggt cattagttca
      241 tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc ctggctgacc
      301 gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag taacgccaat
      361 agggactttc cattgacgtc aatgggtgga gtatttacgg taaactgccc acttggcagt
      421 acatcaagtg tatcatatgc caagtccgcc ccctattgac gtcaatgacg gtaaatggcc
      481 cgcctggcat tatgcccagt acatgacctt acgggacttt cctacttggc agtacatcta
      541 cgtattagtc atcgctatta ccatggtgat gcggttttgg cagtacacca atgggcgtgg
      601 atagcggttt gactcacggg gatttccaag tctccacccc attgacgtca atgggagttt
      661 gttttggcac caaaatcaac gggactttcc aaaatgtcgt aataaccccg ccccgttgac
      721 gcaaatgggc ggtaggcgtg tacggtggga ggtctatata agcagagctc gtttagtgaa
      781 ccgtcagatc actagaagct ttattgcggt agtttatcac agttaaattg ctaacgcagt
      841 cagtgcttct gacacaacag tctcgaactt aagctgcaga agttggtcgt gaggcactgg
      901 gcaggtaagt atcaaggtta caagacaggt ttaaggagac caatagaaac tgggcttgtc
      961 gagacagaga agactcttgc gtttctgata ggcacctatt ggtcttactg acatccactt
     1021 tgcctttctc tccacaggtg tccactccca gttcaattac agctcttaag gctagagtac
     1081 ttaatacgac tcactatagg ctagccacca tgtactagat ggtgtctaag ggcgaagagc
     1141 tgatcaagga aaatatgcgt atgaaggtgg tcatggaagg ttcggtcaac ggccaccaat
     1201 tcaaatgcac aggtgaagga gaaggcagac cgtacgaggg aactcaaacc atgaggatca
     1261 aagtcatcga gggaggaccc ctgccatttg cctttgacat tcttgccacg tcgttcatgt
     1321 atggcagccg tacttttatc aagtacccgg ccgacatccc tgatttcttt aaacagtcct
     1381 ttcctgaggg ttttacttgg gaaagagtta cgagatacga agatggtgga gtcgtcaccg
     1441 tcacgcagga caccagcctt gaggatggcg agctcgtcta caacgtcaag gtcagagggg
     1501 taaactttcc ctccaatggt cccgtgatgc agaagaagac caagggttgg gagcctaata
     1561 cagagatgat gtatccagca gatggtggtc tgagaggata cactgacatc gcactgaaag
     1621 ttgatggtgg tggccatctg cactgcaact tcgtgacaac ttacaggtca aaaaagaccg
     1681 tcgggaacat caagatgccc ggtgtccatg ccgttgatca ccgcctggaa aggatcgagg
     1741 agagtgacaa tgaaacctac gtagtgcaac gcgaagtggc agttgccaaa tacagcaacc
     1801 ttggtggtgg catggacgag ctgtacaagt actagagggt accgcgggcc cgggatccac
     1861 cggggccgcg actctagatc ataatcagcc ataccacatt tgtagaggtt ttacttgctt
     1921 taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     1981 ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     2041 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     2101 cttatactag agcctcagac ccttttagtc agtgtggaaa atctctagca gt
//