BBa_M50047
1
BBa_M50047
Linear DNA to be used with HIV I Integrase pD649
2016-12-08T12:00:00Z
2016-12-10T07:55:19Z
source
long description
false
false
_848_
34393
34393
9
false
design consideration
false
Michelle Bae
component2532446
1
BBa_M50009
component2532448
1
BBa_M50046
component2532440
1
BBa_M50043
component2532445
1
BBa_M50045
component2532449
1
BBa_M50044
annotation2532440
1
BBa_M50043
range2532440
1
1
40
annotation2532449
1
BBa_M50044
range2532449
1
2113
2152
annotation2532448
1
BBa_M50046
range2532448
1
1838
2104
annotation2532445
1
BBa_M50045
range2532445
1
49
1112
annotation2532446
1
BBa_M50009
range2532446
1
1119
1829
BBa_M50045
1
BBa_M50045
hCMV Promoter and Kozak
2016-12-08T12:00:00Z
2016-12-08T06:09:34Z
n/a
The hCMV promoter is usually conducted in mammalian cells, as it is a strong promoter and drives constitutive expression of genes under its control. The kozak sequence here plays a major role in the translation process for eukaryotes. The elements in between help with overall transcription.
false
false
_848_
34430
34430
9
false
n/a
false
Mark Buckup
annotation2531765
1
Start
range2531765
1
1062
1064
annotation2531764
1
Kozak
range2531764
1
1053
1061
annotation2531762
1
hCMV promoter
range2531762
1
1
797
annotation2531763
1
T7 Promoter
range2531763
1
1034
1052
BBa_M50009
1
BBa_M50009
mRuby3 fluorescent protein
2016-11-29T12:00:00Z
2016-12-11T04:21:38Z
http://www.nature.com/articles/srep20889
mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in FRET analysis as an acceptor fluorophore when paired with a GFP such as cometGFP.
false
false
_848_
34390
34390
9
false
choosing to put it in our custom part of the plasmid rather than GFP as we had originally intended
false
Amanda Urke
annotation2532561
1
Deletion of stop codon to integrate subsequent parts of construct
range2532561
1
711
711
BBa_M50044
1
BBa_M50044
HIV-1 U5 Region
2016-12-08T12:00:00Z
2016-12-08T05:31:14Z
2006. HIV-1, complete genome. GenBank:AF033819.3.
This sequence is the terminal 40 base pairs of the HIV-1 U5 region. It is recognized by HIV-1 integrase at the 3' end of the HIV genome prior to integration.
false
false
_848_
34431
34431
9
false
None
false
Michael Herschl
BBa_M50046
1
BBa_M50046
SV40 polyA Terminator
2016-12-08T12:00:00Z
2016-12-08T05:39:24Z
n/a
PolyA terminator, used in mammalian cells specifically, with unknown filler regions.
false
false
_848_
34430
34430
9
false
n/a
false
Mark Buckup
annotation2531721
1
SV40 polyA terminator
range2531721
1
146
267
BBa_M50043
1
BBa_M50043
HIV-1 U3 Region
2016-12-08T12:00:00Z
2016-12-08T05:28:28Z
2006. HIV-1, complete genome. GenBank:AF033819.3.
This sequence is the terminal 40 base pairs of the HIV-1 U3 region. It is recognized by HIV-1 integrase at the 5' end of the HIV genome prior to integration.
false
false
_848_
34431
34431
9
false
None
false
Michael Herschl
BBa_M50043_sequence
1
actggaagggctaattcactcccaaagaagacaagatatc
BBa_M50044_sequence
1
cctcagacccttttagtcagtgtggaaaatctctagcagt
BBa_M50045_sequence
1
tcaatattggccattagccatattattcattggttatatagcataaatcaatattggctattggccattgcatacgttgtatctatatcataatatgtacatttatattggctcatgtccaatatgaccgccatgttggcattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcactagaagctttattgcggtagtttatcacagttaaattgctaacgcagtcagtgcttctgacacaacagtctcgaacttaagctgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaagacaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagactcttgcgtttctgataggcacctattggtcttactgacatccactttgcctttctctccacaggtgtccactcccagttcaattacagctcttaaggctagagtacttaatacgactcactataggctagccaccatg
BBa_M50046_sequence
1
ggtaccgcgggcccgggatccaccggggccgcgactctagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatctta
BBa_M50009_sequence
1
atggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggtggtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggagaaggcagaccgtacgagggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccggccgacatccctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcacgcaggacaccagccttgaggatggcgagctcgtctacaacgtcaaggtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgagaggatacactgacatcgcactgaaagttgatggtggtggccatctgcactgcaacttcgtgacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtgtccatgccgttgatcaccgcctggaaaggatcgaggagagtgacaatgaaacctacgtagtgcaacgcgaagtggcagttgccaaatacagcaaccttggtggtggcatggacgagctgtacaag
BBa_M50047_sequence
1
actggaagggctaattcactcccaaagaagacaagatatctactagagtcaatattggccattagccatattattcattggttatatagcataaatcaatattggctattggccattgcatacgttgtatctatatcataatatgtacatttatattggctcatgtccaatatgaccgccatgttggcattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcactagaagctttattgcggtagtttatcacagttaaattgctaacgcagtcagtgcttctgacacaacagtctcgaacttaagctgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaagacaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagactcttgcgtttctgataggcacctattggtcttactgacatccactttgcctttctctccacaggtgtccactcccagttcaattacagctcttaaggctagagtacttaatacgactcactataggctagccaccatgtactagatggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggtggtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggagaaggcagaccgtacgagggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccggccgacatccctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcacgcaggacaccagccttgaggatggcgagctcgtctacaacgtcaaggtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgagaggatacactgacatcgcactgaaagttgatggtggtggccatctgcactgcaacttcgtgacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtgtccatgccgttgatcaccgcctggaaaggatcgaggagagtgacaatgaaacctacgtagtgcaacgcgaagtggcagttgccaaatacagcaaccttggtggtggcatggacgagctgtacaagtactagagggtaccgcgggcccgggatccaccggggccgcgactctagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatactagagcctcagacccttttagtcagtgtggaaaatctctagcagt
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z