BBa_M50047 1 BBa_M50047 Linear DNA to be used with HIV I Integrase pD649 2016-12-08T12:00:00Z 2016-12-10T07:55:19Z source long description false false _848_ 34393 34393 9 false design consideration false Michelle Bae component2532446 1 BBa_M50009 component2532448 1 BBa_M50046 component2532440 1 BBa_M50043 component2532445 1 BBa_M50045 component2532449 1 BBa_M50044 annotation2532440 1 BBa_M50043 range2532440 1 1 40 annotation2532449 1 BBa_M50044 range2532449 1 2113 2152 annotation2532448 1 BBa_M50046 range2532448 1 1838 2104 annotation2532445 1 BBa_M50045 range2532445 1 49 1112 annotation2532446 1 BBa_M50009 range2532446 1 1119 1829 BBa_M50045 1 BBa_M50045 hCMV Promoter and Kozak 2016-12-08T12:00:00Z 2016-12-08T06:09:34Z n/a The hCMV promoter is usually conducted in mammalian cells, as it is a strong promoter and drives constitutive expression of genes under its control. The kozak sequence here plays a major role in the translation process for eukaryotes. The elements in between help with overall transcription. false false _848_ 34430 34430 9 false n/a false Mark Buckup annotation2531765 1 Start range2531765 1 1062 1064 annotation2531764 1 Kozak range2531764 1 1053 1061 annotation2531762 1 hCMV promoter range2531762 1 1 797 annotation2531763 1 T7 Promoter range2531763 1 1034 1052 BBa_M50009 1 BBa_M50009 mRuby3 fluorescent protein 2016-11-29T12:00:00Z 2016-12-11T04:21:38Z http://www.nature.com/articles/srep20889 mRuby3 is a brighter and more photostable variant of a red fluorescent protein developed by Bryce T. Bajar et al. According to their February 2016 study, mRuby3 has a maximum excitation wavelength of 558 nm, and a maximum emission wavelength of 592 nm. It can be used in FRET analysis as an acceptor fluorophore when paired with a GFP such as cometGFP. false false _848_ 34390 34390 9 false choosing to put it in our custom part of the plasmid rather than GFP as we had originally intended false Amanda Urke annotation2532561 1 Deletion of stop codon to integrate subsequent parts of construct range2532561 1 711 711 BBa_M50044 1 BBa_M50044 HIV-1 U5 Region 2016-12-08T12:00:00Z 2016-12-08T05:31:14Z 2006. HIV-1, complete genome. GenBank:AF033819.3. This sequence is the terminal 40 base pairs of the HIV-1 U5 region. It is recognized by HIV-1 integrase at the 3' end of the HIV genome prior to integration. false false _848_ 34431 34431 9 false None false Michael Herschl BBa_M50046 1 BBa_M50046 SV40 polyA Terminator 2016-12-08T12:00:00Z 2016-12-08T05:39:24Z n/a PolyA terminator, used in mammalian cells specifically, with unknown filler regions. false false _848_ 34430 34430 9 false n/a false Mark Buckup annotation2531721 1 SV40 polyA terminator range2531721 1 146 267 BBa_M50043 1 BBa_M50043 HIV-1 U3 Region 2016-12-08T12:00:00Z 2016-12-08T05:28:28Z 2006. HIV-1, complete genome. GenBank:AF033819.3. This sequence is the terminal 40 base pairs of the HIV-1 U3 region. It is recognized by HIV-1 integrase at the 5' end of the HIV genome prior to integration. false false _848_ 34431 34431 9 false None false Michael Herschl BBa_M50043_sequence 1 actggaagggctaattcactcccaaagaagacaagatatc BBa_M50044_sequence 1 cctcagacccttttagtcagtgtggaaaatctctagcagt BBa_M50045_sequence 1 tcaatattggccattagccatattattcattggttatatagcataaatcaatattggctattggccattgcatacgttgtatctatatcataatatgtacatttatattggctcatgtccaatatgaccgccatgttggcattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcactagaagctttattgcggtagtttatcacagttaaattgctaacgcagtcagtgcttctgacacaacagtctcgaacttaagctgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaagacaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagactcttgcgtttctgataggcacctattggtcttactgacatccactttgcctttctctccacaggtgtccactcccagttcaattacagctcttaaggctagagtacttaatacgactcactataggctagccaccatg BBa_M50046_sequence 1 ggtaccgcgggcccgggatccaccggggccgcgactctagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatctta BBa_M50009_sequence 1 atggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggtggtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggagaaggcagaccgtacgagggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccggccgacatccctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcacgcaggacaccagccttgaggatggcgagctcgtctacaacgtcaaggtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgagaggatacactgacatcgcactgaaagttgatggtggtggccatctgcactgcaacttcgtgacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtgtccatgccgttgatcaccgcctggaaaggatcgaggagagtgacaatgaaacctacgtagtgcaacgcgaagtggcagttgccaaatacagcaaccttggtggtggcatggacgagctgtacaag BBa_M50047_sequence 1 actggaagggctaattcactcccaaagaagacaagatatctactagagtcaatattggccattagccatattattcattggttatatagcataaatcaatattggctattggccattgcatacgttgtatctatatcataatatgtacatttatattggctcatgtccaatatgaccgccatgttggcattgattattgactagttattaatagtaatcaattacggggtcattagttcatagcccatatatggagttccgcgttacataacttacggtaaatggcccgcctggctgaccgcccaacgacccccgcccattgacgtcaataatgacgtatgttcccatagtaacgccaatagggactttccattgacgtcaatgggtggagtatttacggtaaactgcccacttggcagtacatcaagtgtatcatatgccaagtccgccccctattgacgtcaatgacggtaaatggcccgcctggcattatgcccagtacatgaccttacgggactttcctacttggcagtacatctacgtattagtcatcgctattaccatggtgatgcggttttggcagtacaccaatgggcgtggatagcggtttgactcacggggatttccaagtctccaccccattgacgtcaatgggagtttgttttggcaccaaaatcaacgggactttccaaaatgtcgtaataaccccgccccgttgacgcaaatgggcggtaggcgtgtacggtgggaggtctatataagcagagctcgtttagtgaaccgtcagatcactagaagctttattgcggtagtttatcacagttaaattgctaacgcagtcagtgcttctgacacaacagtctcgaacttaagctgcagaagttggtcgtgaggcactgggcaggtaagtatcaaggttacaagacaggtttaaggagaccaatagaaactgggcttgtcgagacagagaagactcttgcgtttctgataggcacctattggtcttactgacatccactttgcctttctctccacaggtgtccactcccagttcaattacagctcttaaggctagagtacttaatacgactcactataggctagccaccatgtactagatggtgtctaagggcgaagagctgatcaaggaaaatatgcgtatgaaggtggtcatggaaggttcggtcaacggccaccaattcaaatgcacaggtgaaggagaaggcagaccgtacgagggaactcaaaccatgaggatcaaagtcatcgagggaggacccctgccatttgcctttgacattcttgccacgtcgttcatgtatggcagccgtacttttatcaagtacccggccgacatccctgatttctttaaacagtcctttcctgagggttttacttgggaaagagttacgagatacgaagatggtggagtcgtcaccgtcacgcaggacaccagccttgaggatggcgagctcgtctacaacgtcaaggtcagaggggtaaactttccctccaatggtcccgtgatgcagaagaagaccaagggttgggagcctaatacagagatgatgtatccagcagatggtggtctgagaggatacactgacatcgcactgaaagttgatggtggtggccatctgcactgcaacttcgtgacaacttacaggtcaaaaaagaccgtcgggaacatcaagatgcccggtgtccatgccgttgatcaccgcctggaaaggatcgaggagagtgacaatgaaacctacgtagtgcaacgcgaagtggcagttgccaaatacagcaaccttggtggtggcatggacgagctgtacaagtactagagggtaccgcgggcccgggatccaccggggccgcgactctagatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatactagagcctcagacccttttagtcagtgtggaaaatctctagcagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z