BBa_M50050 1 BBa_M50050 IPTG-Inducible LuxS Expression in E. coli for Controlling Growth Rates 2016-12-11T12:00:00Z 2016-12-12T01:31:50Z The source of the LuxS gene came from UniProt. All other parts were provided through DNA 2.0 under the plasmid name PD441-CH. When transfected into E. coli, this plasmid will augment it's ability to express the LuxS synthase protein, allowing for differing production of Autoinducer-2. The expression of this plasmid is controlled by inducing with IPTG, allowing for artificial control of increased AI-2 levels. false false _848_ 34327 34327 9 false Designing this part required no modifications. false Anthony Agbay component2532477 1 BBa_B0010 component2532475 1 BBa_M50027 component2532474 1 BBa_J61100 component2532476 1 BBa_K133133 component2532473 1 BBa_K592008 annotation2532477 1 BBa_B0010 range2532477 1 709 788 annotation2532476 1 BBa_K133133 range2532476 1 680 700 annotation2532474 1 BBa_J61100 range2532474 1 135 146 annotation2532473 1 BBa_K592008 range2532473 1 1 126 annotation2532475 1 BBa_M50027 range2532475 1 153 671 BBa_K133133 1 His-Stop His affinity tag-stop 2008-10-22T11:00:00Z 2015-05-08T01:09:57Z Part was constructed by primer annealing. This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector. false false _231_ 0 1883 9 It's complicated false false Katja Kolar BBa_J61100 1 BBa_J61100 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A {{JCA_Arkin_RBSFamily}} false true _95_ 0 483 95 In stock false N/A true John Anderson BBa_K592008 1 PT5lac T5-lac Promoter 2011-09-10T11:00:00Z 2015-05-08T01:12:48Z Phage T5 Released HQ 2013 T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase. false false _763_ 0 7929 9 In stock false The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter. false Erik Lundin annotation2135656 1 lacO1 site range2135656 1 1 26 annotation2135657 1 lacO1 site range2135657 1 69 86 annotation2135658 1 lacOi site range2135658 1 94 123 annotation2135659 1 -35 signal range2135659 1 63 68 annotation2135661 1 -10 signal range2135661 1 87 93 annotation2127135 1 T5-lac promoter range2127135 1 1 126 BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M50027 1 BBa_M50027 LuxS Synthase for Autoinducer-2 Pathway in E. coli 2016-11-28T12:00:00Z 2016-12-12T01:37:34Z The sequence was sourced from Uniprot under the name P45578 (LUXS_ECOLI). This part is an unmodified LuxS synthase protein. It allows for the synthesis of Auto-Inducer 2, a quorum sensing molecule allowing for inter- and intra-species communication of cell density, etc. false false _848_ 34327 34327 9 false There were no modifications of the original genomic sequence. false Anthony Agbay, Tyler Dao, Vivek Lam BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_M50027_sequence 1 atgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgcagttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctagtag BBa_J61100_sequence 1 aaagaggggaca BBa_M50050_sequence 1 tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagaaagaggggacatactagatgccgttgttagatagcttcacagtcgatcatacccggatggaagcgcctgcagttcgggtggcgaaaacaatgaacaccccgcatggcgacgcaatcaccgtgttcgatctgcgcttctgcgtgccgaacaaagaagtgatgccagaaagagggatccataccctggagcacctgtttgctggttttatgcgtaaccatcttaacggtaatggtgtagagattatcgatatctcgccaatgggctgccgcaccggtttttatatgagtctgattggtacgccagatgagcagcgtgttgctgatgcctggaaagcggcaatggaagacgtgctgaaagtgcaggatcagaatcagatcccggaactgaacgtctaccagtgtggcacttaccagatgcactcgttgcaggaagcgcaggatattgcgcgtagcattctggaacgtgacgtacgcatcaacagcaacgaagaactggcactgccgaaagagaagttgcaggaactgcacatctagtagtactagagcaccaccaccaccaccactagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K133133_sequence 1 caccaccaccaccaccactag BBa_K592008_sequence 1 tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z