BBa_K133133
1
His-Stop
His affinity tag-stop
2008-10-22T11:00:00Z
2015-05-08T01:09:57Z
Part was constructed by primer annealing.
This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector.
false
false
_231_
0
1883
9
It's complicated
false
false
Katja Kolar
BBa_J61100
1
BBa_J61100
Ribosome Binding Site Family Member
2007-01-28T12:00:00Z
2015-08-31T02:03:00Z
N/A
{{JCA_Arkin_RBSFamily}}
false
true
_95_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_M50051
1
BBa_M50051
IPTG Inducible Plasmid for the Expression of LsrR Repressor
2016-12-11T12:00:00Z
2016-12-11T03:30:39Z
The genetic sequence of the LsrR gene was sourced through the UniProt database. Other parts were sourced through DNA 2.0 under the plasmid name pD444-CH.
This plasmid augments the natural expression of the LsrR repressor for the lsr operon. It is controlled through the induction of IPTG, allowing for close control of artificial expression.
false
false
_848_
34327
34327
9
false
We did not run into any major design considerations after analyzing our construct using Gene Designer.
false
Anthony Agbay
component2532489
1
BBa_B0010
component2532488
1
BBa_K133133
component2532485
1
BBa_K592008
component2532487
1
BBa_M50028
component2532486
1
BBa_J61100
annotation2532488
1
BBa_K133133
range2532488
1
1118
1138
annotation2532487
1
BBa_M50028
range2532487
1
153
1109
annotation2532485
1
BBa_K592008
range2532485
1
1
126
annotation2532486
1
BBa_J61100
range2532486
1
135
146
annotation2532489
1
BBa_B0010
range2532489
1
1147
1226
BBa_M50028
1
BBa_M50028
LsrR Repressor Protein for the Autoinducer-2 Pathway in E. coli
2016-11-28T12:00:00Z
2016-12-12T01:38:28Z
The genetic sequence was sourced from Uniprot under the name P76141 (LSRR_ECOLI).
This is the functional LsrR repressor protein for the autoinducer-2 pathway in E. coli. It represses the Lsr operon, preventing the intake of autoinducer-2 into the cell.
false
false
_848_
34327
34327
9
false
WE did not have to make any alterations of the original DNA sequence.
false
Anthony Agbay
BBa_K592008
1
PT5lac
T5-lac Promoter
2011-09-10T11:00:00Z
2015-05-08T01:12:48Z
Phage T5
Released HQ 2013
T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase.
false
false
_763_
0
7929
9
In stock
false
The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter.
false
Erik Lundin
annotation2135657
1
lacO1 site
range2135657
1
69
86
annotation2135659
1
-35 signal
range2135659
1
63
68
annotation2135661
1
-10 signal
range2135661
1
87
93
annotation2135656
1
lacO1 site
range2135656
1
1
26
annotation2127135
1
T5-lac promoter
range2127135
1
1
126
annotation2135658
1
lacOi site
range2135658
1
94
123
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_J61100_sequence
1
aaagaggggaca
BBa_M50051_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagaaagaggggacatactagatgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggatatattagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatagtactagagcaccaccaccaccaccactagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K133133_sequence
1
caccaccaccaccaccactag
BBa_M50028_sequence
1
atgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggatatattagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatag
BBa_K592008_sequence
1
tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z