BBa_K133133 1 His-Stop His affinity tag-stop 2008-10-22T11:00:00Z 2015-05-08T01:09:57Z Part was constructed by primer annealing. This is His-tag with stop codon. His-tag is used for detection of His-tagged proteins by anti-His antibodies. Cloned into pSB1AK3 vector. false false _231_ 0 1883 9 It's complicated false false Katja Kolar BBa_M50051 1 BBa_M50051 IPTG Inducible Plasmid for the Expression of LsrR Repressor 2016-12-11T12:00:00Z 2016-12-11T03:30:39Z The genetic sequence of the LsrR gene was sourced through the UniProt database. Other parts were sourced through DNA 2.0 under the plasmid name pD444-CH. This plasmid augments the natural expression of the LsrR repressor for the lsr operon. It is controlled through the induction of IPTG, allowing for close control of artificial expression. false false _848_ 34327 34327 9 false We did not run into any major design considerations after analyzing our construct using Gene Designer. false Anthony Agbay component2532485 1 BBa_K592008 component2532487 1 BBa_M50028 component2532486 1 BBa_J61100 component2532489 1 BBa_B0010 component2532488 1 BBa_K133133 annotation2532489 1 BBa_B0010 range2532489 1 1147 1226 annotation2532488 1 BBa_K133133 range2532488 1 1118 1138 annotation2532486 1 BBa_J61100 range2532486 1 135 146 annotation2532485 1 BBa_K592008 range2532485 1 1 126 annotation2532487 1 BBa_M50028 range2532487 1 153 1109 BBa_J61100 1 BBa_J61100 Ribosome Binding Site Family Member 2007-01-28T12:00:00Z 2015-08-31T02:03:00Z N/A {{JCA_Arkin_RBSFamily}} false true _95_ 0 483 95 In stock false N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_M50028 1 BBa_M50028 LsrR Repressor Protein for the Autoinducer-2 Pathway in E. coli 2016-11-28T12:00:00Z 2016-12-12T01:38:28Z The genetic sequence was sourced from Uniprot under the name P76141 (LSRR_ECOLI). This is the functional LsrR repressor protein for the autoinducer-2 pathway in E. coli. It represses the Lsr operon, preventing the intake of autoinducer-2 into the cell. false false _848_ 34327 34327 9 false WE did not have to make any alterations of the original DNA sequence. false Anthony Agbay BBa_K592008 1 PT5lac T5-lac Promoter 2011-09-10T11:00:00Z 2015-05-08T01:12:48Z Phage T5 Released HQ 2013 T5-lac promoter is a hybrid promoter made from the phage T5 early promoter and lac-operon. It contains three LacI binding sites and remains repressed in LacIq strains where LacI is expressed in high levels. It is inducible by IPTG, and recognizable by E.coli RNA polymerase. Compared to the T7 promoters, T5-lac promoter doesn't require the co-expression of phage polymerase. false false _763_ 0 7929 9 In stock false The most common DH5alpha and TOP10 used in assembly are not LacI-constitutive (LacIq) strains. Thus, any ORF placed downstream to this promoter will be massively transcribed. Therefore, it's recommended to employ low-copy number plasmids or even LacIq strains when adding genes to this promoter. false Erik Lundin annotation2135657 1 lacO1 site range2135657 1 69 86 annotation2135659 1 -35 signal range2135659 1 63 68 annotation2135658 1 lacOi site range2135658 1 94 123 annotation2135656 1 lacO1 site range2135656 1 1 26 annotation2135661 1 -10 signal range2135661 1 87 93 annotation2127135 1 T5-lac promoter range2127135 1 1 126 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_J61100_sequence 1 aaagaggggaca BBa_M50051_sequence 1 tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacgtactagagaaagaggggacatactagatgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggatatattagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatagtactagagcaccaccaccaccaccactagtactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K133133_sequence 1 caccaccaccaccaccactag BBa_M50028_sequence 1 atgacaatcaacgattcggcaatttcagaacagggaatgtgtgaagaagaacaggtcgcgcggatcgcgtggttttactatcacgacgggctgacccagagcgagatcagcgatcgtctcggcctgacacgtttgaaagtgtcgcgattgctggagaaagggcatcagtccggcattattcgcgtacagattaattctcgctttgaaggctgtctggaatatgaaactcaattacgtcgtcagttttcgctgcaacatgtccgggtgatccctgggcttgcggatgctgatgtcggtgggcgactggggataggcgcggcgcatatgttgatgagtttacttcaaccacaacagatgctggcgattggttttggcgaggcaaccatgaatacgctgcaacgcttaagtggttttatttcgtcacagcaaattcgcctggtcacgctctccggtggcgtcggttcttatatgacgggaatcgggcagcttaacgcggcgtgcagtgtgaatattattccggctccgttgcgggcatcctccgctgacattgcccgtacgctaaaaaatgaaaattgcgtcaaagatgttctgttagccgcgcaagcagcggatgtggcgattgtcggcattggtgctgtgagtcaacaggacgatgcgacaatcattcgctccggatatattagccagggcgaacagttaatgattggccgaaaaggggcggttggcgacattttaggctacttttttgatgcaaaaggtgacgttgtcacgaatatcaaaatacataacgaactgattggcttacctttaagcgcgctgaagaccatacccgtccgggttggcgtggcagggggagaaaataaagccgaagcaattgccgctgcaatgaaaggcggttatatcaacgcactggttaccgatcaggacacagcagcggcgattttacgtagttaatag BBa_K592008_sequence 1 tgtggaattgtgagcggataacaattacgagcttcatgcacagtgaaatcatgaaaaatttatttgctttgtgagcggataacaattataatatgtggaattgtgagcgctcacaattccacaacg igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z