BBa_M50052 1 BBa_M50052 alkB1 + alkG 2016-12-11T12:00:00Z 2016-12-11T04:20:42Z Sourced from genome of A. borkumensis bacterium Composite Part containing both alkB1 (ABO-2707) and alkG (ABO-2708) sequences for alkane degradation (BBa_M50004 and BBa_M50005). In conjunction, these enzymes should add an -OH group to an alkane of up to thirty-two carbons in size. false false _848_ 34388 34388 9 false Refer to individual design considerations for BBa_M50005 and BBa_M50004 false Nicholas M Kuntz component2532557 1 BBa_M50004 component2532560 1 BBa_M50005 annotation2532560 1 BBa_M50005 range2532560 1 1222 1746 annotation2532557 1 BBa_M50004 range2532557 1 1 1215 BBa_M50004 1 BBa_M50004 alkB1 2016-10-26T11:00:00Z 2016-12-01T04:47:08Z Prevalent in marine bacteria, like Alcanivorax Borkumensis. alkB1 works with alkG in Alcanivorax Borkumensis to begin the process of breaking down alkanes in oils for marine bacteria. Specifically, the alkB1 and alkG genes work in combination to add an oxygen molecule to one end of the alkane chain. false false _848_ 34389 34389 9 false Removed repeat sequences of 10 or more bases. false Ryan Buchanan annotation2531661 1 Mutation 2: T to G range2531661 1 606 606 annotation2531660 1 Mutation 1: C to T range2531660 1 183 183 BBa_M50005 1 BBa_M50005 alkG 2016-10-26T11:00:00Z 2016-12-10T11:04:33Z alkG comes from the genome of Alcanivorax Borkumensis. alkG plays a role in the chemical transformation of alkanes to make oils easier for marine bacteria, like A. Borkumensis to digest. This gene, in combination with other genes like alkB1 in A. Borkumensis's genome, break down any alkanes with up to 32 carbons into molecules with carboxyl groups, which these bacteria are able to digest. false false _848_ 34388 34395 9 false We changed the repeat sequences in the DNA of this gene and plan to optimize this gene for expression in E. Coli. false Addie Petersen annotation2531662 1 Mutation 1: G to T range2531662 1 207 207 annotation2531663 1 Mutation 2: A to G range2531663 1 294 294 BBa_M50052_sequence 1 atgtcagagaacattttaaccgagcccccacgaagtgatgctgataatgagggttatgtggaccgaaagcgccacttgtggattctttctgtactgtggccagcaacaccaataattggcctatatctcgtatcccaaacagggtggagtatatggtacggattagtattaatcctatggtatggcctagtccctttgatcgacaccatgcttggcgaggattattccaaccctcctgaatccgttgttcctaagcttgaacaagaccgttactacaaagttttaacgtacttaaccgttcctattcattatgcagcgttgattataagtgcctggtgggtatctacccaaccaataggggtattcgagtttttagctctcgccctttctttgggcattgttaatggactggcactcaacacaggccatgaactcgggcataaaaaagaaacctttgaccgctggatggccaagcttgtactggccgtggtcggatatggtcacttcttcattgaacacaataaagggcatcatcgtgacgtagcaacaccgatggacccggctacatcccgcatgggcgaatctatttatacgttttcactgcgggaaattcctggtgcctttaaacgggcatggggcttggaagagcagcgcctcagccgttgcggcaaaagcgtatggagcctagataatgaagtcttacagcctatgattttgacggtagtgctttatgccgcattgctggcatttttcggtcctttaatgctcatctttttgcccattcaaatggccttcggctggtggcagctgaccagtgccaattatattgagcactacggactgctgcgtgaaaagctgccgaacgggcgttacgagcatcaaaaaccccatcattcatggaattcaaaccatgtaatgtcgaacctcatcctgtttcatctgcaacgtcattcagatcaccatgcgcatcctacaagatcttatcaatcacttagggacttcagcgaccttcccaccctgcctacgggctaccctgggatgttcttcgtcgcattctttccctcctggtttcgttcactaatggatgatcgggtgatggagtgggcgcacggagacattaataagatccagattcagccgggaatgcgtgaattctatgagcaaaaatttggagtaaagggttcggagtcacccgatacaaccgttgccaaataatactagatggctaaatatcaatgccccgattgcgaatatatatacgatgaagtcgctggccacccacacgaaggcttccccccaggaacgtcttgggaaacgattcctgaagagtgggcctgcccagactgtgcagtaagggataaagctgacttcgtagtaatagaatccggttccgcgtccccggcgtctggcgcggccaccccagaagttcgcactgctaccaccccacctaaggcagaggcttcacctcaaaaatcaacgggggcctcgactccttcagctaacaataaagccaaggcaaaggctaaagccaaacccgcacgggcaaaatcgtctaaagactccaccggcaaagagaccacctttcgtaaatggatctgtatcacttgcggtcacatttatgatgaagctcttggcgatgaaactgaagggttcgcgccaggcactctttttgaagatatcccggacgattggtgctgtcccgactgtggtgccacaaaagaggactatgtcctccatgaagattag BBa_M50005_sequence 1 atggctaaatatcaatgccccgattgcgaatatatatacgatgaagtcgctggccacccacacgaaggcttccccccaggaacgtcttgggaaacgattcctgaagagtgggcctgcccagactgtgcagtaagggataaagctgacttcgtagtaatagaatccggttccgcgtccccggcgtctggcgcggccaccccagaagttcgcactgctaccaccccacctaaggcagaggcttcacctcaaaaatcaacgggggcctcgactccttcagctaacaataaagccaaggcaaaggctaaagccaaacccgcacgggcaaaatcgtctaaagactccaccggcaaagagaccacctttcgtaaatggatctgtatcacttgcggtcacatttatgatgaagctcttggcgatgaaactgaagggttcgcgccaggcactctttttgaagatatcccggacgattggtgctgtcccgactgtggtgccacaaaagaggactatgtcctccatgaagattag BBa_M50004_sequence 1 atgtcagagaacattttaaccgagcccccacgaagtgatgctgataatgagggttatgtggaccgaaagcgccacttgtggattctttctgtactgtggccagcaacaccaataattggcctatatctcgtatcccaaacagggtggagtatatggtacggattagtattaatcctatggtatggcctagtccctttgatcgacaccatgcttggcgaggattattccaaccctcctgaatccgttgttcctaagcttgaacaagaccgttactacaaagttttaacgtacttaaccgttcctattcattatgcagcgttgattataagtgcctggtgggtatctacccaaccaataggggtattcgagtttttagctctcgccctttctttgggcattgttaatggactggcactcaacacaggccatgaactcgggcataaaaaagaaacctttgaccgctggatggccaagcttgtactggccgtggtcggatatggtcacttcttcattgaacacaataaagggcatcatcgtgacgtagcaacaccgatggacccggctacatcccgcatgggcgaatctatttatacgttttcactgcgggaaattcctggtgcctttaaacgggcatggggcttggaagagcagcgcctcagccgttgcggcaaaagcgtatggagcctagataatgaagtcttacagcctatgattttgacggtagtgctttatgccgcattgctggcatttttcggtcctttaatgctcatctttttgcccattcaaatggccttcggctggtggcagctgaccagtgccaattatattgagcactacggactgctgcgtgaaaagctgccgaacgggcgttacgagcatcaaaaaccccatcattcatggaattcaaaccatgtaatgtcgaacctcatcctgtttcatctgcaacgtcattcagatcaccatgcgcatcctacaagatcttatcaatcacttagggacttcagcgaccttcccaccctgcctacgggctaccctgggatgttcttcgtcgcattctttccctcctggtttcgttcactaatggatgatcgggtgatggagtgggcgcacggagacattaataagatccagattcagccgggaatgcgtgaattctatgagcaaaaatttggagtaaagggttcggagtcacccgatacaaccgttgccaaataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z