BBa_M50052
1
BBa_M50052
alkB1 + alkG
2016-12-11T12:00:00Z
2016-12-11T04:20:42Z
Sourced from genome of A. borkumensis bacterium
Composite Part containing both alkB1 (ABO-2707) and alkG (ABO-2708) sequences for alkane degradation (BBa_M50004 and BBa_M50005). In conjunction, these enzymes should add an -OH group to an alkane of up to thirty-two carbons in size.
false
false
_848_
34388
34388
9
false
Refer to individual design considerations for BBa_M50005 and BBa_M50004
false
Nicholas M Kuntz
component2532557
1
BBa_M50004
component2532560
1
BBa_M50005
annotation2532560
1
BBa_M50005
range2532560
1
1222
1746
annotation2532557
1
BBa_M50004
range2532557
1
1
1215
BBa_M50004
1
BBa_M50004
alkB1
2016-10-26T11:00:00Z
2016-12-01T04:47:08Z
Prevalent in marine bacteria, like Alcanivorax Borkumensis.
alkB1 works with alkG in Alcanivorax Borkumensis to begin the process of breaking down alkanes in oils for marine bacteria. Specifically, the alkB1 and alkG genes work in combination to add an oxygen molecule to one end of the alkane chain.
false
false
_848_
34389
34389
9
false
Removed repeat sequences of 10 or more bases.
false
Ryan Buchanan
annotation2531661
1
Mutation 2: T to G
range2531661
1
606
606
annotation2531660
1
Mutation 1: C to T
range2531660
1
183
183
BBa_M50005
1
BBa_M50005
alkG
2016-10-26T11:00:00Z
2016-12-10T11:04:33Z
alkG comes from the genome of Alcanivorax Borkumensis.
alkG plays a role in the chemical transformation of alkanes to make oils easier for marine bacteria, like A. Borkumensis to digest. This gene, in combination with other genes like alkB1 in A. Borkumensis's genome, break down any alkanes with up to 32 carbons into molecules with carboxyl groups, which these bacteria are able to digest.
false
false
_848_
34388
34395
9
false
We changed the repeat sequences in the DNA of this gene and plan to optimize this gene for expression in E. Coli.
false
Addie Petersen
annotation2531662
1
Mutation 1: G to T
range2531662
1
207
207
annotation2531663
1
Mutation 2: A to G
range2531663
1
294
294
BBa_M50052_sequence
1
atgtcagagaacattttaaccgagcccccacgaagtgatgctgataatgagggttatgtggaccgaaagcgccacttgtggattctttctgtactgtggccagcaacaccaataattggcctatatctcgtatcccaaacagggtggagtatatggtacggattagtattaatcctatggtatggcctagtccctttgatcgacaccatgcttggcgaggattattccaaccctcctgaatccgttgttcctaagcttgaacaagaccgttactacaaagttttaacgtacttaaccgttcctattcattatgcagcgttgattataagtgcctggtgggtatctacccaaccaataggggtattcgagtttttagctctcgccctttctttgggcattgttaatggactggcactcaacacaggccatgaactcgggcataaaaaagaaacctttgaccgctggatggccaagcttgtactggccgtggtcggatatggtcacttcttcattgaacacaataaagggcatcatcgtgacgtagcaacaccgatggacccggctacatcccgcatgggcgaatctatttatacgttttcactgcgggaaattcctggtgcctttaaacgggcatggggcttggaagagcagcgcctcagccgttgcggcaaaagcgtatggagcctagataatgaagtcttacagcctatgattttgacggtagtgctttatgccgcattgctggcatttttcggtcctttaatgctcatctttttgcccattcaaatggccttcggctggtggcagctgaccagtgccaattatattgagcactacggactgctgcgtgaaaagctgccgaacgggcgttacgagcatcaaaaaccccatcattcatggaattcaaaccatgtaatgtcgaacctcatcctgtttcatctgcaacgtcattcagatcaccatgcgcatcctacaagatcttatcaatcacttagggacttcagcgaccttcccaccctgcctacgggctaccctgggatgttcttcgtcgcattctttccctcctggtttcgttcactaatggatgatcgggtgatggagtgggcgcacggagacattaataagatccagattcagccgggaatgcgtgaattctatgagcaaaaatttggagtaaagggttcggagtcacccgatacaaccgttgccaaataatactagatggctaaatatcaatgccccgattgcgaatatatatacgatgaagtcgctggccacccacacgaaggcttccccccaggaacgtcttgggaaacgattcctgaagagtgggcctgcccagactgtgcagtaagggataaagctgacttcgtagtaatagaatccggttccgcgtccccggcgtctggcgcggccaccccagaagttcgcactgctaccaccccacctaaggcagaggcttcacctcaaaaatcaacgggggcctcgactccttcagctaacaataaagccaaggcaaaggctaaagccaaacccgcacgggcaaaatcgtctaaagactccaccggcaaagagaccacctttcgtaaatggatctgtatcacttgcggtcacatttatgatgaagctcttggcgatgaaactgaagggttcgcgccaggcactctttttgaagatatcccggacgattggtgctgtcccgactgtggtgccacaaaagaggactatgtcctccatgaagattag
BBa_M50005_sequence
1
atggctaaatatcaatgccccgattgcgaatatatatacgatgaagtcgctggccacccacacgaaggcttccccccaggaacgtcttgggaaacgattcctgaagagtgggcctgcccagactgtgcagtaagggataaagctgacttcgtagtaatagaatccggttccgcgtccccggcgtctggcgcggccaccccagaagttcgcactgctaccaccccacctaaggcagaggcttcacctcaaaaatcaacgggggcctcgactccttcagctaacaataaagccaaggcaaaggctaaagccaaacccgcacgggcaaaatcgtctaaagactccaccggcaaagagaccacctttcgtaaatggatctgtatcacttgcggtcacatttatgatgaagctcttggcgatgaaactgaagggttcgcgccaggcactctttttgaagatatcccggacgattggtgctgtcccgactgtggtgccacaaaagaggactatgtcctccatgaagattag
BBa_M50004_sequence
1
atgtcagagaacattttaaccgagcccccacgaagtgatgctgataatgagggttatgtggaccgaaagcgccacttgtggattctttctgtactgtggccagcaacaccaataattggcctatatctcgtatcccaaacagggtggagtatatggtacggattagtattaatcctatggtatggcctagtccctttgatcgacaccatgcttggcgaggattattccaaccctcctgaatccgttgttcctaagcttgaacaagaccgttactacaaagttttaacgtacttaaccgttcctattcattatgcagcgttgattataagtgcctggtgggtatctacccaaccaataggggtattcgagtttttagctctcgccctttctttgggcattgttaatggactggcactcaacacaggccatgaactcgggcataaaaaagaaacctttgaccgctggatggccaagcttgtactggccgtggtcggatatggtcacttcttcattgaacacaataaagggcatcatcgtgacgtagcaacaccgatggacccggctacatcccgcatgggcgaatctatttatacgttttcactgcgggaaattcctggtgcctttaaacgggcatggggcttggaagagcagcgcctcagccgttgcggcaaaagcgtatggagcctagataatgaagtcttacagcctatgattttgacggtagtgctttatgccgcattgctggcatttttcggtcctttaatgctcatctttttgcccattcaaatggccttcggctggtggcagctgaccagtgccaattatattgagcactacggactgctgcgtgaaaagctgccgaacgggcgttacgagcatcaaaaaccccatcattcatggaattcaaaccatgtaatgtcgaacctcatcctgtttcatctgcaacgtcattcagatcaccatgcgcatcctacaagatcttatcaatcacttagggacttcagcgaccttcccaccctgcctacgggctaccctgggatgttcttcgtcgcattctttccctcctggtttcgttcactaatggatgatcgggtgatggagtgggcgcacggagacattaataagatccagattcagccgggaatgcgtgaattctatgagcaaaaatttggagtaaagggttcggagtcacccgatacaaccgttgccaaataa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z