LOCUS BBa_M50053 2320 bp DNA linear 27-Dec-2024 DEFINITION FRET-based glucose sensor using a glucose binding protein, mRuby3 and cometGFP ACCESSION BBa_M50053 VERSION BBa_M50053.1 FEATURES Location/Qualifiers promoter 1..48 /label=BBa_M50031 RBS 49..61 /label=BBa_M36771 misc_feature 772..772 /label=Deletion of stop codon to integrate subsequent parts of construct misc_feature 62..772 /label=BBa_M50009 misc_feature 773..790 /label=BBa_M50036 misc_feature 791..791 /label=Deletion of start codon to facilitate fusion to linker and fluorophore misc_feature 2029..2029 /label=Deletion of stop codon to facilitate fusion to linker and fluorophore misc_feature 791..2029 /label=BBa_M50037 misc_feature 2030..2047 /label=BBa_M50036 misc_feature 2048..2320 /label=BBa_M36936 ORIGIN 1 aaatcatgaa aaatttattt gctttgtgag cggataacaa ttataataag gaggtaaaaa 61 aatggtgtct aagggcgaag agctgatcaa ggaaaatatg cgtatgaagg tggtcatgga 121 aggttcggtc aacggccacc aattcaaatg cacaggtgaa ggagaaggca gaccgtacga 181 gggaactcaa accatgagga tcaaagtcat cgagggagga cccctgccat ttgcctttga 241 cattcttgcc acgtcgttca tgtatggcag ccgtactttt atcaagtacc cggccgacat 301 ccctgatttc tttaaacagt cctttcctga gggttttact tgggaaagag ttacgagata 361 cgaagatggt ggagtcgtca ccgtcacgca ggacaccagc cttgaggatg gcgagctcgt 421 ctacaacgtc aaggtcagag gggtaaactt tccctccaat ggtcccgtga tgcagaagaa 481 gaccaagggt tgggagccta atacagagat gatgtatcca gcagatggtg gtctgagagg 541 atacactgac atcgcactga aagttgatgg tggtggccat ctgcactgca acttcgtgac 601 aacttacagg tcaaaaaaga ccgtcgggaa catcaagatg cccggtgtcc atgccgttga 661 tcaccgcctg gaaaggatcg aggagagtga caatgaaacc tacgtagtgc aacgcgaagt 721 ggcagttgcc aaatacagca accttggtgg tggcatggac gagctgtaca aggggggcac 781 cggcgggtcc cgcaagtggt tacttgcaat cggcatggtg ctgggtttaa gtgccttagc 841 ccaaggggga aagcttgaaa tttttagttg gtgggctggc gacgagggcc ctgcgctgga 901 agcgcttatc cgtttgtata aacagaagta cccaggtgta gaggttatta atgccacggt 961 aacgggcggt gcgggtgtga acgcacgtgc ggtgttgaaa acacgcatgt taggaggcga 1021 cccaccagat actttccaag tgcatgcggg aatggagttg attggcacat gggtcgtcgc 1081 aaaccgtatg gaggacctgt cggctttatt ccgtcaggaa ggatggttgc aggcatttcc 1141 taagggattg attgacctga tctcttacaa aggcggaatc tggtcagtcc ccgttaatat 1201 ccaccgcagc aacgtcatgt ggtaccttcc tgctaagttg aaagagtggg gtgtaaaccc 1261 acctcgtaca tgggacgaat ttttggccac ttgtcaaaca ttaaagcaaa agggtttaga 1321 agcacccctt gcattgggcg aaaattggac ccagcagcat ctgtgggaat cagtggcctt 1381 ggcggtattg ggtccagatg actggaataa tctttggaac gggaagttga agtttacgga 1441 ccccaaggca gttcgcgctt gggaggtatt cggtcgcgta cttgactgtg cgaataaaga 1501 tgccgctggg ctgagctggc aacaagcggt agatcgtgta gtacaaggaa aagccgcttt 1561 caacgtgatg ggggactggg ccgcagggta tatgaccacg accctgaagt tgaagccggg 1621 caccgacttc gcttgggccc cttctccggg tactcaagga gtttttatga tgctgtcgga 1681 ctcctttgga cttccaaaag gagctaagaa tcgtcaaaac gctatcaatt ggttgcgttt 1741 agtgggttcg aaagagggac aagacacatt taacccatta aagggttcca ttgcagcgcg 1801 tttggatagc gatccatcca agtacaatgc atatgggcag tccgccatgc gtgattggcg 1861 ttctaatcgt atcgttggct cgttggtcca cggtgcggtc gctccagagt cattcatgtc 1921 tcagttcggc acggtaatgg aaatctttct tcagacacgc aacccgcagg ccgccgcgaa 1981 cgctgctcag gccattgcgg atcaggtggg actgggccgc ttggggcagg ggggcaccgg 2041 cgggtccgcg tgctgcggct gcaccggcgc gggctgcgcg gcgaccgcgg cgtgcaccgc 2101 gggctgcgcg accgcggcgt gctgctgctg caccaccggc ggcggcggct gctgcacctg 2161 caccgcggcg gcgtgcggcg gcggcacctg caccaccggc gcgggcggcg gcggcaccac 2221 caccaccacc accggctgca ccggcgcggc ggcgggcggc gcgggcggcg cggcgtgcac 2281 cgcgaccgcg acctgctgcg gcggcggcac cgcggcgtgc //