LOCUS       BBa_M50054               894 bp    DNA     linear       01-Jul-2024
DEFINITION  PETase double mutant I208V and R90A
ACCESSION   BBa_M50054
VERSION     BBa_M50054.1  
FEATURES             Location/Qualifiers
     misc_feature    1..3
                     /label=start codon
     misc_feature    4..873
                     /label=PETase coding region
     misc_feature    874..891
                     /label=6 hig tag
     misc_feature    892..894
                     /label=stop codon
     misc_feature    622..624
                     /label=I208V
     misc_feature    268..270
                     /label=R90A
ORIGIN
        1 atgaactttc cccgcgcttc ccgtcttatg caggcggctg tgttgggcgg attaatggcg
       61 gtcagcgcgg cggcaacggc tcagacaaat ccttatgcac gcggccctaa tcccactgcc
      121 gcttcccttg aagctagtgc aggccctttc accgttcgct cgtttaccgt gtcccgcccg
      181 tccgggtatg gcgcggggac ggtatattat ccgacaaatg ccggcggaac cgtcggtgct
      241 atcgctatcg tgcctggcta cactgctgcc cagtcctcaa ttaaatggtg gggaccgcgt
      301 ttagcaagcc atggttttgt cgtaattact atcgatacta attcgaccct tgaccaaccg
      361 tcctctcgca gcagtcagca gatggcggct cttcgtcaag ttgcaagctt gaatgggaca
      421 tcaagctcgc cgatttatgg taaggtggat accgcgcgta tgggcgttat gggctggtcg
      481 atgggcggag gtgggtcgtt gatttctgcg gccaacaacc ccagtcttaa agctgctgcc
      541 ccccaagcgc cctgggactc tagtacgaac ttcagttccg tcacagtgcc tacattaatc
      601 tttgcgtgcg agaatgacag cgtagccccg gtgaattcaa gcgccttacc gatctacgat
      661 agtatgtccc gtaatgctaa acagttttta gagattaacg gggggtccca ctcgtgtgct
      721 aattctggca actcgaatca ggctctgatt ggcaaaaaag gtgttgcctg gatgaaacgc
      781 tttatggaca atgacactcg ctactcgacg ttcgcttgtg agaaccccaa tagtacgcgt
      841 gtcagtgatt tccgcaccgc gaactgcagt caccatcatc accatcatta gtag
//