BBa_M50055
1
BBa_M50055
Atrazine biosensor
2016-12-11T12:00:00Z
2016-12-12T12:34:30Z
.
This is an atrazine biosensor that reflects the presence of atrazine through GFP fluorescence. A UTR in the riboswitch causes excessive base pairing in the RNA transcript, folding the RNA such that the RBS is not normally exposed. When atrazine is present, it binds to the aptamer sequence and alters the shape of the RNA to allow binding at the RBS, thus allowing translation of a GFP sequence following the RBS. The level of GFP expression corresponds to the level of atrazine in the surrounding environment.
false
false
_848_
34438
34438
9
false
.
false
Eleanor Glockner
annotation2532644
1
RBS
range2532644
1
91
111
annotation2532642
1
BBa_M50048
range2532642
1
1
111
annotation2532645
1
eGFP
range2532645
1
112
842
annotation2532643
1
atrazine aptamer
range2532643
1
18
90
annotation2532641
1
5' UTR
range2532641
1
1
17
BBa_M50055_sequence
1
gactcactataggtacctgtaccgtctgagcgattcgtacgaacggctttgtactgtttgcactggcagccagtcagtgttaaggagtgcccctgctaaggtaacaacaagatgaaatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z