BBa_P100107 1 BBa_P100107 EHT1 ORF with RBS 2009-06-28T11:00:00Z 2015-05-08T01:14:11Z PCR EHT1 ORF with RBS false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2006841 1 BBa_B0034 component2006842 1 BBa_C100007 annotation2006841 1 BBa_B0034 range2006841 1 1 12 annotation2006842 1 BBa_C100007 range2006842 1 19 1374 BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_C100007 1 BBa_C100007 ORF of the EHT1 odorant enzyme 2009-06-24T11:00:00Z 2015-08-31T04:07:24Z Amplified via PCR from S. cerevisiae. Requires SDM to remove one EcoRI site. ORF of the EHT1 odorant enzyme false false _11_ 0 1380 102 Not in stock false Added biobricks prefix and suffix. Removed EcoRI site through SDM. false Matt Gethers BBa_B0034_sequence 1 aaagaggagaaa BBa_C100007_sequence 1 atgtcagaagtttccaaatggccagcaatcaacccattccattggggatacaatggtacagtttcgcatattgtcggtgaaaatggttccattaaactccatttaaaagacaacaaggagcaagttgattttgacgagttcgctaacaaatatgtcccaacgttgaagaatggtgcccaattcaaattgagtccttacttgttcacaggtattttgcaaactttgtacttaggtgctgctgatttctctaagaaatttcctgtattctacggcagggaaattgtcaaattctcggatggtggagtttgcaccgctgactggctcatagattcatggaaaaaggattatgaatttgatcaaagtactacgagctttgataaaaaaaaatttgataaagacgagaaggcgacacatccagaaggatggcctcgtttacaaccacgtacaaggtacctgaaagataatgagttggaagaactacgggaggttgatctacccctagtagttattctacatggtcttgctggtggtagtcatgagccgattataagatctcttgctgaaaacctgtctcgcagtgggagatttcaagtggtcgtcctaaataccagaggttgtgcacgttccaaaattaccaccagaaatttatttacagcttatcacacaatggatattcgcgagtttttgcaaagagaaaagcaaagacatccagatagaaaactatacgctgtgggatgctcttttggtgctacgatgctggcaaactatctgggagaagagggcgataaatcacctttatccgcagctgctactttgtgcaatccttgggatcttctcctttcagcaattaggatgagccaggattggtggtcaagaactttattttccaaaaatattgcgcaattcttaacaagaaccgttcaggttaatatgggtgaattaggagttccaaatggctctctccccgatcatcctcccacagtcaagaatccatctttctatatgttcacgcctgaaaatctaataaaggcaaagagctttaaatcgacccgggaatttgatgaagtgtacactgcgcctgctttaggcttcccaaatgctatggagtattataaagcggccagctcaataaacagagttgatacaattcgggttcctacccttgttatcaattccagggatgatcctgttgtcggcccagatcaaccatactcaatcgtggaaaagaatcctcgtattttgtattgtagaaccgatttaggtggtcatttagcttacctagataaagacaacaactcgtgggctaccaaggcaattgcagaatttttcactaagtttgatgaattagtcgtatga BBa_P100107_sequence 1 aaagaggagaaatactagatgtcagaagtttccaaatggccagcaatcaacccattccattggggatacaatggtacagtttcgcatattgtcggtgaaaatggttccattaaactccatttaaaagacaacaaggagcaagttgattttgacgagttcgctaacaaatatgtcccaacgttgaagaatggtgcccaattcaaattgagtccttacttgttcacaggtattttgcaaactttgtacttaggtgctgctgatttctctaagaaatttcctgtattctacggcagggaaattgtcaaattctcggatggtggagtttgcaccgctgactggctcatagattcatggaaaaaggattatgaatttgatcaaagtactacgagctttgataaaaaaaaatttgataaagacgagaaggcgacacatccagaaggatggcctcgtttacaaccacgtacaaggtacctgaaagataatgagttggaagaactacgggaggttgatctacccctagtagttattctacatggtcttgctggtggtagtcatgagccgattataagatctcttgctgaaaacctgtctcgcagtgggagatttcaagtggtcgtcctaaataccagaggttgtgcacgttccaaaattaccaccagaaatttatttacagcttatcacacaatggatattcgcgagtttttgcaaagagaaaagcaaagacatccagatagaaaactatacgctgtgggatgctcttttggtgctacgatgctggcaaactatctgggagaagagggcgataaatcacctttatccgcagctgctactttgtgcaatccttgggatcttctcctttcagcaattaggatgagccaggattggtggtcaagaactttattttccaaaaatattgcgcaattcttaacaagaaccgttcaggttaatatgggtgaattaggagttccaaatggctctctccccgatcatcctcccacagtcaagaatccatctttctatatgttcacgcctgaaaatctaataaaggcaaagagctttaaatcgacccgggaatttgatgaagtgtacactgcgcctgctttaggcttcccaaatgctatggagtattataaagcggccagctcaataaacagagttgatacaattcgggttcctacccttgttatcaattccagggatgatcctgttgtcggcccagatcaaccatactcaatcgtggaaaagaatcctcgtattttgtattgtagaaccgatttaggtggtcatttagcttacctagataaagacaacaactcgtgggctaccaaggcaattgcagaatttttcactaagtttgatgaattagtcgtatga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z