BBa_P100115 1 BBa_P100115 LIS ORF with RBS (optimized) 2009-07-09T11:00:00Z 2015-05-08T01:14:11Z Synthesis LIS ORF with RBS (optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2010848 1 BBa_B0034 component2010849 1 BBa_C100015 annotation2010848 1 BBa_B0034 range2010848 1 1 12 annotation2010849 1 BBa_C100015 range2010849 1 19 2631 BBa_C100015 1 BBa_C100015 LIS ORF (B0034 optimized) 2009-07-09T11:00:00Z 2015-08-31T04:07:25Z Synthesis LIS ORF (B0034 optimized) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0034_sequence 1 aaagaggagaaa BBa_C100015_sequence 1 atgcagctgattacgaacttcagcagcagctctagcgaactgcaattcctggttgacaaagttaaacgtgaatctctgtcctcctcctccagcaacactcaaaacctgtttctgtccacctctccgtacgacactgcatggctggcgctgatcccgcaccctcaccaccaccaccatcacggccgtccaatgtttgagaaatgcctgcaatggatcctgcacaaccagaccccgcaaggcttttgggcagctgctggcgacaacatctccgatactgacgacgatgtaaccctggactgcctgctgagcaccctggcctgtctggtcgcgctgaaacgctggcagctggctccggatatgatccataaaggtctggagtttgtgaatcgcaacaccgagcgtctggtgatgaaacagaaaccgtccgatgttccgcgctggtttactatcatgttcccggctatgctggagctggctggcgcttctagcctgcgtgtagatttctctgaaaacctgaaccgtatcctggttgaactgagccagaatcgtgacgatatcctgactcgtgaagaagtggatgaaaagaaacagtatagcccgctgctgctgttcctggaagcgctgccggctcagtcctacgacaatgatgtgctgaagcagattattgataagaacctgagcaacgacggttccctgctgcaatctccgtctgcgactgcccgtgcttatatgatcactggtaatacccgttgcctgtcttacctgcattctctgaccaactcttgcagcaacggcggtgttccatccttctacccggtggatgacgacctgcatgatctggttatggttaaccaactgacccgtagcggtctgactgaacacctgattccagaaatcgatcacctgctgctgaaagtgcagaaaaactacaaatacaagaaagcgagcccgaaaagcctgtacagcattgctgctgagctgtaccgcgattctctggccttctggctgctgcgtgtcaacaaccactgggtttctccgtctatcttttgctggtttctggatgacgacgaaattcgtgaccacattgaaaccaactacgaggaatttgctgccgtgctgctgaatgtttaccgtgcaacggacctgatgttttccggtgaggtgcagctggtcgaagcccgttccttcgcgactaaaaacctggaaaaaattctggcaaccggtaacatccataaaactaacgcggatatttctagctctctgcacaaaatgattgagcacgagctgcgcgtcccgtggacggcgcgtatggaccatgtcgaaaaccgcatttggatcgaagagatcgcatccagcgcactgtggttcggtaaaagctcttacctgcgcctgagctgcttccataaaatgagcctgcaacagctggcggttaaaaactatactctgcgtcagctggtgtatcgtgacgaactggcggaagttgaacgctggtccaaagagcgtggcctgtgcgacatgggtttctgccgtgagaaaaccggctactgttactacgcttttgctgcaagcacctgtctgccgtggagctctgacgtccgtctggttctgactaaagcagctgttgtaattaccgtggcagacgacttcttcgatgtggagggtagcatggtggacctggaaaaactgacggacgcagttcgtcgttgggatgcggaaggcctgggctctcactctaagactatcttcgaagctctggatgacctggtaaacgaagtgcgcctgaaatgtttccaacagaacggtcaggacatcaaaaacaacctgcaacaactgtggtacgaaaccttccactcttggctgatggaagctaaatggggcaaaggtctgacctccaaaccgagcgttgacgtgtacctgggtaacgctatgacgtctattgcagctcataccatggtgctgactgcaagctgtctgctgggtcctggttttccagtgcaccagctgtggtcccagcgccgccaccaggatatcacctccctgctgatggtactgactcgcctgctgaacgacatccagtcttacctgaaagaagaagatgaaggtaagattaactacgtgtggatgtacatgattgaaaacaaccaagccagcatcgacgattctgttcgccacgtgcagactatcatcaacgttaaaaagcaggaatttatccaacgtgttctgtccgatcagcactgcaacctgccgaaaagcttcaagcaactgcacttctcttgcctgaaagtgttcaacatgtttttcaactcttctaacatcttcgatacggacaccgatctgctgctggatatccacgaagcgtttgtgtctccacctcaggtcccgaaattcaaaccgcacatcaaaccaccgcaccagctgccggccactctgcaaccgccacaccagccgcagcagattatggttaacaagaagaaagtggaaatggtttacaaatcttatcaccatccgttcaaggtatttaccctgcaaaagaaacagtcctctggtcacggtaccatgaatcctcgtgcgtctatcctggctggtccaaacatcaaactgtgtttcagctaa BBa_P100115_sequence 1 aaagaggagaaatactagatgcagctgattacgaacttcagcagcagctctagcgaactgcaattcctggttgacaaagttaaacgtgaatctctgtcctcctcctccagcaacactcaaaacctgtttctgtccacctctccgtacgacactgcatggctggcgctgatcccgcaccctcaccaccaccaccatcacggccgtccaatgtttgagaaatgcctgcaatggatcctgcacaaccagaccccgcaaggcttttgggcagctgctggcgacaacatctccgatactgacgacgatgtaaccctggactgcctgctgagcaccctggcctgtctggtcgcgctgaaacgctggcagctggctccggatatgatccataaaggtctggagtttgtgaatcgcaacaccgagcgtctggtgatgaaacagaaaccgtccgatgttccgcgctggtttactatcatgttcccggctatgctggagctggctggcgcttctagcctgcgtgtagatttctctgaaaacctgaaccgtatcctggttgaactgagccagaatcgtgacgatatcctgactcgtgaagaagtggatgaaaagaaacagtatagcccgctgctgctgttcctggaagcgctgccggctcagtcctacgacaatgatgtgctgaagcagattattgataagaacctgagcaacgacggttccctgctgcaatctccgtctgcgactgcccgtgcttatatgatcactggtaatacccgttgcctgtcttacctgcattctctgaccaactcttgcagcaacggcggtgttccatccttctacccggtggatgacgacctgcatgatctggttatggttaaccaactgacccgtagcggtctgactgaacacctgattccagaaatcgatcacctgctgctgaaagtgcagaaaaactacaaatacaagaaagcgagcccgaaaagcctgtacagcattgctgctgagctgtaccgcgattctctggccttctggctgctgcgtgtcaacaaccactgggtttctccgtctatcttttgctggtttctggatgacgacgaaattcgtgaccacattgaaaccaactacgaggaatttgctgccgtgctgctgaatgtttaccgtgcaacggacctgatgttttccggtgaggtgcagctggtcgaagcccgttccttcgcgactaaaaacctggaaaaaattctggcaaccggtaacatccataaaactaacgcggatatttctagctctctgcacaaaatgattgagcacgagctgcgcgtcccgtggacggcgcgtatggaccatgtcgaaaaccgcatttggatcgaagagatcgcatccagcgcactgtggttcggtaaaagctcttacctgcgcctgagctgcttccataaaatgagcctgcaacagctggcggttaaaaactatactctgcgtcagctggtgtatcgtgacgaactggcggaagttgaacgctggtccaaagagcgtggcctgtgcgacatgggtttctgccgtgagaaaaccggctactgttactacgcttttgctgcaagcacctgtctgccgtggagctctgacgtccgtctggttctgactaaagcagctgttgtaattaccgtggcagacgacttcttcgatgtggagggtagcatggtggacctggaaaaactgacggacgcagttcgtcgttgggatgcggaaggcctgggctctcactctaagactatcttcgaagctctggatgacctggtaaacgaagtgcgcctgaaatgtttccaacagaacggtcaggacatcaaaaacaacctgcaacaactgtggtacgaaaccttccactcttggctgatggaagctaaatggggcaaaggtctgacctccaaaccgagcgttgacgtgtacctgggtaacgctatgacgtctattgcagctcataccatggtgctgactgcaagctgtctgctgggtcctggttttccagtgcaccagctgtggtcccagcgccgccaccaggatatcacctccctgctgatggtactgactcgcctgctgaacgacatccagtcttacctgaaagaagaagatgaaggtaagattaactacgtgtggatgtacatgattgaaaacaaccaagccagcatcgacgattctgttcgccacgtgcagactatcatcaacgttaaaaagcaggaatttatccaacgtgttctgtccgatcagcactgcaacctgccgaaaagcttcaagcaactgcacttctcttgcctgaaagtgttcaacatgtttttcaactcttctaacatcttcgatacggacaccgatctgctgctggatatccacgaagcgtttgtgtctccacctcaggtcccgaaattcaaaccgcacatcaaaccaccgcaccagctgccggccactctgcaaccgccacaccagccgcagcagattatggttaacaagaagaaagtggaaatggtttacaaatcttatcaccatccgttcaaggtatttaccctgcaaaagaaacagtcctctggtcacggtaccatgaatcctcgtgcgtctatcctggctggtccaaacatcaaactgtgtttcagctaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z