BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_P101104 1 BBa_P101104 JMT RBS.ORF.Term (Synth) 2009-06-28T11:00:00Z 2015-05-08T01:14:11Z Synthesis/Cloning JMT RBS.ORF.Term (Synth) false false _11_ 0 1380 102 Not in stock false None false Matt Gethers component2006894 1 BBa_B0010 component2006893 1 BBa_C100004 component2006896 1 BBa_B0012 component2006892 1 BBa_B0034 annotation2006896 1 BBa_B0012 range2006896 1 1285 1325 annotation2006894 1 BBa_B0010 range2006894 1 1197 1276 annotation2006892 1 BBa_B0034 range2006892 1 1 12 annotation2006893 1 BBa_C100004 range2006893 1 19 1188 BBa_C100004 1 BBa_C100004 ORF of JMT odorant enzyme (Synthesized) 2009-06-24T11:00:00Z 2015-08-31T04:07:24Z Synthesized ORF of JMT odorant enzyme (Synthesized) false false _11_ 0 1380 102 Not in stock false Added biobricks prefix and suffix. false Matt Gethers BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1687 1 stop range1687 1 34 34 annotation1690 1 polya range1690 1 28 41 BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_C100004_sequence 1 atggaagtaatgcgcgtactgcacatgaataagggtaacggtgaaacctcttatgctaagaacagcactgcgcagtccaatatcatctctctgggccgtcgtgttatggatgaagctctgaagaaactgatgatgtctaacagcgaaatctcttccatcggtatcgcagacctgggttgttcttctggcccaaactccctgctgtctattagcaacatcgttgatacgattcacaatctgtgcccggatctggatcgcccagtcccggaactgcgtgtttctctgaacgatctgccgagcaacgattttaactatatctgcgctagcctgccggagttctatgaccgtgttaataacaacaaagaaggtctgggctttggtcgtggcggtggtgaatcctgttttgtgtccgcggttccgggtagcttctacggccgcctgttcccgcgtcgttccctgcacttcgtccactcttcctcctctctgcattggctgtcccaggtaccgtgtcgtgaagcagaaaaagaagaccgtaccattacggcggacctggaaaacatgggcaagatctacatctctaagacctcccctaaaagcgcacacaaggcttacgctctgcaatttcagaccgatttcctggtgtttctgcgctcccgttctgaagaactggtcccgggtggtcgtatggtcctgtctttcctgggccgtcgctctctggatcctaccactgaggaatcttgttatcagtgggaactgctggcccaggcgctgatgtctatggcgaaagaaggcattattgaagaagaaaaaattgacgctttcaacgccccgtactatgcagcgagctccgaagagctgaaaatggtcatcgagaaagagggctctttctccatcgatcgcctggaaatctctccgatcgactgggaaggtggttccatctccgaagaatcctacgacctggcaattcgctctaaaccagaagctctggcgtctggccgccgtgtgagcaacactattcgtgccgttgtggaacctatgctggaaccgacctttggcgagaacgttatggacgagctgttcgaacgttacgcaaagatcgtgggcgagtacttctacgtttcttcccctcgttacgccattgtaattctgtctctggtgcgtaccggttga BBa_B0034_sequence 1 aaagaggagaaa BBa_P101104_sequence 1 aaagaggagaaatactagatggaagtaatgcgcgtactgcacatgaataagggtaacggtgaaacctcttatgctaagaacagcactgcgcagtccaatatcatctctctgggccgtcgtgttatggatgaagctctgaagaaactgatgatgtctaacagcgaaatctcttccatcggtatcgcagacctgggttgttcttctggcccaaactccctgctgtctattagcaacatcgttgatacgattcacaatctgtgcccggatctggatcgcccagtcccggaactgcgtgtttctctgaacgatctgccgagcaacgattttaactatatctgcgctagcctgccggagttctatgaccgtgttaataacaacaaagaaggtctgggctttggtcgtggcggtggtgaatcctgttttgtgtccgcggttccgggtagcttctacggccgcctgttcccgcgtcgttccctgcacttcgtccactcttcctcctctctgcattggctgtcccaggtaccgtgtcgtgaagcagaaaaagaagaccgtaccattacggcggacctggaaaacatgggcaagatctacatctctaagacctcccctaaaagcgcacacaaggcttacgctctgcaatttcagaccgatttcctggtgtttctgcgctcccgttctgaagaactggtcccgggtggtcgtatggtcctgtctttcctgggccgtcgctctctggatcctaccactgaggaatcttgttatcagtgggaactgctggcccaggcgctgatgtctatggcgaaagaaggcattattgaagaagaaaaaattgacgctttcaacgccccgtactatgcagcgagctccgaagagctgaaaatggtcatcgagaaagagggctctttctccatcgatcgcctggaaatctctccgatcgactgggaaggtggttccatctccgaagaatcctacgacctggcaattcgctctaaaccagaagctctggcgtctggccgccgtgtgagcaacactattcgtgccgttgtggaacctatgctggaaccgacctttggcgagaacgttatggacgagctgttcgaacgttacgcaaagatcgtgggcgagtacttctacgtttcttcccctcgttacgccattgtaattctgtctctggtgcgtaccggttgatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z