LOCUS       BBa_P101114             1955 bp    DNA     linear       22-Dec-2024
DEFINITION  LMS ORF with RBS and Term (optimized)
ACCESSION   BBa_P101114
VERSION     BBa_P101114.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..1818
                     /label=BBa_C100014
     stem_loop       1838..1881
                     /label=stem_loop
     misc_feature    1827..1906
                     /label=BBa_B0010
     terminator      1827..1906
                     /label=BBa_B0010
     stem_loop       1922..1941
                     /label=T7 TE
     misc_feature    1948..1948
                     /label=stop
     polyA_site      1942..1955
                     /label=polya
     misc_feature    1915..1955
                     /label=BBa_B0012
     terminator      1915..1955
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat ggcgctgaaa gttctgtcag tcgcgaccca aatggcgatc
       61 ccttctaacc tgactacttg ccttcagccg agccacttca agtccagccc gaaactgctt
      121 tccagcacca atagtagcag ccgtagccgt ctgcgtgtgt actgttctag cagccagctg
      181 accaccgaac gtcgtagcgg taactacaac ccaagccgtt gggatgttaa ctttatccaa
      241 tccctgctgt ccgactataa ggaagacaaa catgtgattc gtgccagtga actggtgacc
      301 ctggttaaaa tggagctgga aaaagaaacc gatcagatcc gtcaactgga actgattgat
      361 gacctgcaac gtatgggctt atcggaccac ttccagaatg aatttaaaga aatcctgtct
      421 tcgatctatt tggaccatca ttattacaaa aacccgttcc cgaaagagga acgtgatctg
      481 tattctacgt ccctggcttt ccgcctgctc cgcgagcacg gctttcaggt ggctcaagaa
      541 gttttcgact ccttcaagaa cgaagaaggg gagttcaaag aatcgctgag cgatgacacc
      601 cgcggcttac tccagctgta tgaagcgagc ttcctgctga ccgaaggcga aactaccctg
      661 gaaagcgcac gcgagtttgc caccaaattt ctcgaagaaa aagtaaatga aggcggtgtc
      721 gatggagatc tgctgacccg cattgcgtat tctctggaca tcccgctgca ctggcgtatc
      781 aaacgtccaa acgcgccggt atggattgag tggtatcgta aacgtccgga catgaaccca
      841 gtcgttctgg agctggcaat cctcgatctg aacatcgttc aggcgcagtt ccaggaagaa
      901 ctgaaagaat ctttccgttg gtggcgcaat accggtttcg ttgaaaaact gccattcgct
      961 cgcgaccgtc tggtggagtg ctacttctgg aacactggaa ttattgaacc gcgccagcat
     1021 gcttcagccc gcattatgat gggtaaagtt aacgctctga ttaccgtaat cgatgacatt
     1081 tacgacgtat acggcaccct ggaagaactg gaacagttta ccgatctgat ccgtcgttgg
     1141 gatatcaact cgatcgatca gctgccggac tacatgcagc tgtgcttcct ggctctcaac
     1201 aacttcgttg atgacacgtc ttatgacgtt atgaaggaaa aaggtgtaaa cgttatcccg
     1261 tacctgcgcc agagctgggt tgatctggcg gacaagtata tggtcgaagc acgttggttc
     1321 tacggtggcc ataaaccgtc tctggaagag tatctggaga actcttggca gagcatcagc
     1381 ggcccgtgca tgctcaccca catcttcttc cgtgttaccg attctttcac caaagaaact
     1441 gtggattcgc tgtataaata ccacgatctg gtccgttggt cttctttcgt gctgcgcctg
     1501 gctgacgacc tgggcaccag cgtagaagaa gtcagccgtg gtgatgtacc gaagtcgctg
     1561 caatgttaca tgtctgatta caacgcatct gaagcggaag ctcgcaaaca tgtgaaatgg
     1621 ttgatcgctg aggtttggaa aaagatgaac gccgaacgtg tgtccaaaga tagcccattc
     1681 ggtaaggact ttattggttg cgctgtggac ctgggtcgta tggcacagct gatgtaccac
     1741 aacggtgatg gtcacggcac ccagcacccg atcatccacc aacagatgac gcgcaccctg
     1801 tttgaaccgt tcgcttaata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1861 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     1921 tggctcacct tcgggtgggc ctttctgcgt ttata
//