LOCUS       BBa_P101115             2768 bp    DNA     linear       22-Nov-2024
DEFINITION  LIS ORF with RBS and Term (optimized)
ACCESSION   BBa_P101115
VERSION     BBa_P101115.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     CDS             19..2631
                     /label=BBa_C100015
     stem_loop       2651..2694
                     /label=stem_loop
     misc_feature    2640..2719
                     /label=BBa_B0010
     terminator      2640..2719
                     /label=BBa_B0010
     stem_loop       2735..2754
                     /label=T7 TE
     misc_feature    2761..2761
                     /label=stop
     polyA_site      2755..2768
                     /label=polya
     misc_feature    2728..2768
                     /label=BBa_B0012
     terminator      2728..2768
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gcagctgatt acgaacttca gcagcagctc tagcgaactg
       61 caattcctgg ttgacaaagt taaacgtgaa tctctgtcct cctcctccag caacactcaa
      121 aacctgtttc tgtccacctc tccgtacgac actgcatggc tggcgctgat cccgcaccct
      181 caccaccacc accatcacgg ccgtccaatg tttgagaaat gcctgcaatg gatcctgcac
      241 aaccagaccc cgcaaggctt ttgggcagct gctggcgaca acatctccga tactgacgac
      301 gatgtaaccc tggactgcct gctgagcacc ctggcctgtc tggtcgcgct gaaacgctgg
      361 cagctggctc cggatatgat ccataaaggt ctggagtttg tgaatcgcaa caccgagcgt
      421 ctggtgatga aacagaaacc gtccgatgtt ccgcgctggt ttactatcat gttcccggct
      481 atgctggagc tggctggcgc ttctagcctg cgtgtagatt tctctgaaaa cctgaaccgt
      541 atcctggttg aactgagcca gaatcgtgac gatatcctga ctcgtgaaga agtggatgaa
      601 aagaaacagt atagcccgct gctgctgttc ctggaagcgc tgccggctca gtcctacgac
      661 aatgatgtgc tgaagcagat tattgataag aacctgagca acgacggttc cctgctgcaa
      721 tctccgtctg cgactgcccg tgcttatatg atcactggta atacccgttg cctgtcttac
      781 ctgcattctc tgaccaactc ttgcagcaac ggcggtgttc catccttcta cccggtggat
      841 gacgacctgc atgatctggt tatggttaac caactgaccc gtagcggtct gactgaacac
      901 ctgattccag aaatcgatca cctgctgctg aaagtgcaga aaaactacaa atacaagaaa
      961 gcgagcccga aaagcctgta cagcattgct gctgagctgt accgcgattc tctggccttc
     1021 tggctgctgc gtgtcaacaa ccactgggtt tctccgtcta tcttttgctg gtttctggat
     1081 gacgacgaaa ttcgtgacca cattgaaacc aactacgagg aatttgctgc cgtgctgctg
     1141 aatgtttacc gtgcaacgga cctgatgttt tccggtgagg tgcagctggt cgaagcccgt
     1201 tccttcgcga ctaaaaacct ggaaaaaatt ctggcaaccg gtaacatcca taaaactaac
     1261 gcggatattt ctagctctct gcacaaaatg attgagcacg agctgcgcgt cccgtggacg
     1321 gcgcgtatgg accatgtcga aaaccgcatt tggatcgaag agatcgcatc cagcgcactg
     1381 tggttcggta aaagctctta cctgcgcctg agctgcttcc ataaaatgag cctgcaacag
     1441 ctggcggtta aaaactatac tctgcgtcag ctggtgtatc gtgacgaact ggcggaagtt
     1501 gaacgctggt ccaaagagcg tggcctgtgc gacatgggtt tctgccgtga gaaaaccggc
     1561 tactgttact acgcttttgc tgcaagcacc tgtctgccgt ggagctctga cgtccgtctg
     1621 gttctgacta aagcagctgt tgtaattacc gtggcagacg acttcttcga tgtggagggt
     1681 agcatggtgg acctggaaaa actgacggac gcagttcgtc gttgggatgc ggaaggcctg
     1741 ggctctcact ctaagactat cttcgaagct ctggatgacc tggtaaacga agtgcgcctg
     1801 aaatgtttcc aacagaacgg tcaggacatc aaaaacaacc tgcaacaact gtggtacgaa
     1861 accttccact cttggctgat ggaagctaaa tggggcaaag gtctgacctc caaaccgagc
     1921 gttgacgtgt acctgggtaa cgctatgacg tctattgcag ctcataccat ggtgctgact
     1981 gcaagctgtc tgctgggtcc tggttttcca gtgcaccagc tgtggtccca gcgccgccac
     2041 caggatatca cctccctgct gatggtactg actcgcctgc tgaacgacat ccagtcttac
     2101 ctgaaagaag aagatgaagg taagattaac tacgtgtgga tgtacatgat tgaaaacaac
     2161 caagccagca tcgacgattc tgttcgccac gtgcagacta tcatcaacgt taaaaagcag
     2221 gaatttatcc aacgtgttct gtccgatcag cactgcaacc tgccgaaaag cttcaagcaa
     2281 ctgcacttct cttgcctgaa agtgttcaac atgtttttca actcttctaa catcttcgat
     2341 acggacaccg atctgctgct ggatatccac gaagcgtttg tgtctccacc tcaggtcccg
     2401 aaattcaaac cgcacatcaa accaccgcac cagctgccgg ccactctgca accgccacac
     2461 cagccgcagc agattatggt taacaagaag aaagtggaaa tggtttacaa atcttatcac
     2521 catccgttca aggtatttac cctgcaaaag aaacagtcct ctggtcacgg taccatgaat
     2581 cctcgtgcgt ctatcctggc tggtccaaac atcaaactgt gtttcagcta atactagagc
     2641 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg
     2701 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg
     2761 cgtttata
//